Fabrication and Morphological Control of Nonspherical Alginate Hydrogel Particles.

We report a simple platform for the fabrication of nonspherical alginate hydrogel particles using a dripping method. Hydrogel particles with novel morphologies, such as vortex ring, teardrop, disk, sphere, and mushroom, are fabricated by controlling various parameters. We monitored the deformation process of the hydrogel particles after they penetrated the crosslinking solution using a high-speed camera. Then, we proposed a mechanism showing a unique morphological transformation from a spherical to a disk shape. We demonstrated how controlling the collecting height that causes the drop impact force against the crosslinking solution surface was critical to producing hydrogel particles with these intriguing shapes. In particular, disk-shaped alginate particles show their ability as potential platforms for culturing mouse adrenocortical tumor cells (Y1) and a hippocampal neuronal cell (HT-22). To modify alginate particles, cell-adhesive gelatin is incorporated into the alginate matrix and then alginate particles are coated with poly(allylamine hydrochloride). Two modified alginate particles show good adhesion and proliferation rates on their surfaces. In particular, the hybrid hydrogel particles provide great potential to be developed into promising materials for cell culture, drug delivery, and tissue engineering.

Recent progress in the synthesis of all-aqueous two-phase droplets using microfluidic approaches.

An aqueous two-phase system (ATPS) is a system with liquid-liquid phase separation and shows great potential for the extraction, separation, purification, and enrichment of proteins, membranes, viruses, enzymes, nucleic acids, and other biomolecules because of its simplicity, biocompatibility, and wide applicability [1-4]. The clear aqueous-aqueous interface of ATPSs is highly advantageous for their implementation, therefore making ATPSs a green alternative approach to replace conventional emulsion systems, such as water-in-oil droplets. All aqueous emulsions (water-in-water, w-in-w) hold great promise in the biomedical field as glucose sensors [5] and promising carriers for the encapsulation and release of various biomolecules and nonbiomolecules [6-10]. However, the ultralow interfacial tension between the two phases is a hurdle in generating w-in-w emulsion droplets. In the past, bulk emulsification and electrospray techniques were employed for the generation of w-in-w emulsion droplets and the fabrication of microparticles and microcapsules in the later stage. Bulk emulsification is a simple and low-cost technique; however, it generates polydisperse w-in-w emulsion droplets. Another technique, electrospray, involves easy experimental setups that can generate monodisperse but nonspherical w-in-w emulsion droplets. In comparison, microfluidic platforms provide monodisperse w-in-w emulsion droplets with spherical shapes, deal with the small volumes of solutions and short reaction times and achieve portability and versatility in their design through rapid prototyping. Owing to several advantages, microfluidic approaches have recently been introduced. To date, several different strategies have been explored to generate w-in-w emulsions and multiple w-in-w emulsions and to fabricate microparticles and microcapsules using conventional microfluidic devices. Although a few review articles on ATPSs emulsions have been published in the past, to date, few reviews have exclusively focused on the evolution of microfluidic-based ATPS droplets. The present review begins with a brief discussion of the history of ATPSs and their fundamentals, which is followed by an account chronicling the integration of microfluidic devices with ATPSs to generate w-in-w emulsion droplets. Furthermore, the stabilization strategies of w-in-w emulsion droplets and microfluidic fabrication of microparticles and microcapsules for modern applications, such as biomolecule encapsulation and spheroid construction, are discussed in detail in this review. We believe that the present review will provide useful information to not only new entrants in the microfluidic community wanting to appreciate the findings of the field but also existing researchers wanting to keep themselves updated on progress in the field.

A surfactant-free approach: Novel one-step ultrasonic nebulizer spray method to generate amphiphilic Janus particles.

HYPOTHESIS: A solvent evaporation-induced phase separation method, which is based on the preferential partitioning of two or more immiscible materials after solvent evaporation on providing heat, has been one of the main strategies for synthesis of Janus particles (JPs). Considering this approach, it should be possible to synthesize surfactant free-JPs in continuous flow by the ultrasonic nebulizer spray method. EXPERIMENTS: Two polymers, polystyrene and polymethylmethacrylate, were dissolved in dichloromethane, and droplets of a precursor solution generated by an ultrasonic nebulizer were then conveyed through a borosilicate glass cylinder with two heating zones. The solvent evaporation-induced phase separation occurred in a single flow process, which resulted in the preferential partitioning of two incompatible polymers in the droplets, leading to the formation of the spherical bicompartmental JPs. FINDINGS: The successful fabrication of spherical JPs was observed at high polymer concentrations (1.5 and 2.0 wt%), and at elevated temperature (40-75 °C). The fluorescent compartmentalization of JPs was confirmed. Furthermore, the interfacial arrangement of JPs at oil-water interface was studied. A detailed explanation of theoretical prediction of interfacial configurations of JPs was provided. Lastly, the generated JPs were proved as Pickering stabilizers at the oil-water interface.

Metal-organic frameworks/alginate composite beads as effective adsorbents for the removal of hexavalent chromium from aqueous solution.

Industrial waste discharge comprising heavy metals into potable water bodies induces many health hazards. This study investigates the role of metal-organic frameworks (MOFs) doped alginate beads (MOFs@ABs) as potential adsorbents for Cr(VI). Effects of pH, stirring rate, temperature, initial chrome concentration, and particles dosage on Cr(VI) adsorption are studied to evaluate adsorption ability of UiO-66@ABs for Cr(VI) removal from aqueous solution. The adsorption kinetics follows pseudo second order and the equilibrium isotherm is consistent with Langmuir isotherm model. The maximum adsorption capacity of UiO-66@ABs calculated from the model conforms to the experimental results. The desorption experiment of Cr(VI) adsorbed UiO-66@ABs (82%) demonstrates satisfactory regeneration efficiency. Based on our findings and comparative controlled experiments, the superiority of UiO-66@ABs promises their potential application in Cr(VI) removal from wastewater.