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1.
AAPS PharmSciTech ; 19(7): 2934-2947, 2018 Oct.
Article in English | MEDLINE | ID: mdl-29980982

ABSTRACT

The current paper continues our study on the ability of L-arginine to prevent/reduce the aggregation of proteins that results from the various stresses during the lyophilisation and/or storage of lyophilized protein-based products. The first part of our study, i.e. formulation development, was devoted to the rational design and optimization of an L-arginine containing lyophilized formulation which can resist the natural tendency of L-arginine to absorb atmosphere moisture. Mannitol and trehalose were chosen among other excipients to be included in the protein-based formulation, as mannitol in a combination with L-arginine has been shown to reduce moisture sorption while trehalose provides a degree of lyoprotection. In the present study, a number of formulations, which comprised bovine serum albumin (BSA) with and without L-arginine, and with five different ratios of trehalose-to-mannitol (from 30:70 to 80:20) were lyophilised and assessed. The internal structures and the moisture sorption/retention of the lyophilized formulations were characterised. To study the effect of L-arginine on BSA solid-phase stability, the lyophilized powder was exposed to accelerated storage conditions (high moisture (75% RH) and temperature (22 or 45 °C)) for up to 24 h. The lyophilized BSA formulations were then reconstituted and solution-state protein aggregation assessed by turbidimetry at 360 nm and fluorescence spectroscopy using the thioflavin T assay. It was demonstrated that L-arginine can be used in protein-based freeze-dried formulations to significantly reduce the aggregation of protein during the manufacturing, storage and subsequent reconstitution. The results also revealed the importance of a sufficient amount of mannitol in the arginine-containing formulations.


Subject(s)
Arginine/chemistry , Freeze Drying , Polymers/chemistry , Protein Aggregates , Serum Albumin, Bovine/chemistry , Sugars/chemistry , Animals , Arginine/analysis , Cattle , Drug Stability , Freeze Drying/methods , Polymers/analysis , Serum Albumin, Bovine/analysis , Sugars/analysis
2.
AAPS PharmSciTech ; 19(2): 896-911, 2018 Feb.
Article in English | MEDLINE | ID: mdl-29047017

ABSTRACT

L-arginine was introduced into protein-based freeze-dried formulations to study the ability of arginine to reduce/prevent from protein aggregation during manufacturing, storage and reconstitution of lyophilized protein-based pharmaceuticals. As L-arginine is known to be very hygroscopic, additional excipients which could provide a moisture buffering capacity need to be introduced into the formulation. In the first part of our study-excipient formulation development-the screening of a number of sugars/polyols has been done in order to select the best combination of excipients that, in a complex with L-arginine, can (i) produce freeze-dried cakes with elegant appearance, adequate mechanical properties and reconstitution times, and (ii) resist/minimise the moisture sorption. Various freeze-dried cakes containing L-arginine in combination with mannitol, trehalose, lactose and sucrose were produced and analysed by TGA, DSC, texture analysis, moisture sorption, cake shrinkage, TVIM and SEM. The non-linear dependencies of the physicochemical properties of the freeze-dried cakes on the sugar-to-mannitol ratios were found. The best combinations of excipients (L-arginine, mannitol and trehalose) were selected to be used in the second part of this work, in which the impact of each selected formulation will be studied in relation to the aggregation of a protein.


Subject(s)
Arginine/chemistry , Polymers/chemical synthesis , Protein Aggregates , Proteins/chemical synthesis , Sugars/chemical synthesis , Arginine/metabolism , Drug Compounding , Drug Stability , Freeze Drying/methods , Lactose/chemistry , Lactose/metabolism , Mannitol/chemistry , Mannitol/metabolism , Polymers/metabolism , Protein Aggregates/physiology , Proteins/metabolism , Sucrose/chemistry , Sucrose/metabolism , Sugars/metabolism , Trehalose/chemistry , Trehalose/metabolism
3.
Eur Biophys J ; 44(4): 249-61, 2015 May.
Article in English | MEDLINE | ID: mdl-25794693

ABSTRACT

Thioflavin T (ThT) is a fluorescent dye able to enhance significantly its fluorescence quantum yield upon binding to protein amyloids. ThT assay is widely used to detect and quantify amyloids in a variety of conditions, including solutions with different pH levels. In the present work, the effect of acidic and basic pH on the conformation of the ThT molecule and its absorption and fluorescence properties was studied. The results show that both acidic and basic pH decrease significantly the intensity of ThT absorption in the visible region and fluorescence emission intensity. Low pHs induce an immediate "all-or-nothing" decrease in the ThT signal, while in alkaline solutions the ThT signal decreases gradually over time. pH-induced signal quenching is less in the presence of glycerol or protein aggregates. Two different mechanisms are responsible for the ThT signal quenching-the ThT hydroxylation at basic pH and protonation of the nitrogen atom of the dimethylamino group at acidic pH. ThT assays should be carefully carried out at basic or acidic pH as strong pH dependence of ThT could be responsible for misinterpretation and false positive/negative experimental results. The potential unsuitability of ThT as a probe in solutions with high pH (>9) has been shown.


Subject(s)
Absorption, Radiation , Fluorescent Dyes/chemistry , Thiazoles/chemistry , Benzothiazoles , Fluorescence , Fluorescent Dyes/radiation effects , Hydrogen-Ion Concentration , Thiazoles/radiation effects , Ultraviolet Rays
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