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2.
Clin Microbiol Infect ; 20(3): O142-9, 2014 Mar.
Article in English | MEDLINE | ID: mdl-24520878

ABSTRACT

The exact mode of transmission of leprosy is not clearly understood; however, many studies have demonstrated active transmission of leprosy around a source case. Families of five active leprosy cases and their household contacts were chosen from a high endemic area in Purulia. Fifty-two soil samples were also collected from different areas of their houses. DNA was extracted from slit-skin smears (SSS) and soil samples and the Mycobacterium leprae-specific RLEP (129 bp) region was amplified using PCR. Molecular typing of M. leprae was performed for all RLEP PCR-positive samples by single nucleotide polymorphism (SNP) typing and confirmation by DNA sequencing. SSS of these five patients and six out of the total 28 contacts were PCR positive for RLEP whereas 17 soil samples out of 52 showed the presence of M. leprae DNA. SNP typing of M. leprae from all RLEP PCR-positive subjects (patients and smear-positive contacts) and 10 soil samples showed the SNP type 1 genotype. M. leprae DNA from the five leprosy patients and the six contacts was further subtyped and the D subtype was noted in all patients and contacts, except for one contact where the C subtype was identified. Typing followed by subtyping of M. leprae clearly revealed that either the contacts were infected by the patients or both patients and contacts had the same source of infection. It also revealed that the type of M. leprae in the soil in the inhabited areas where patients resided was also of the same type as that found in patients.


Subject(s)
Genome, Bacterial , Genotype , Leprosy/microbiology , Leprosy/transmission , Mycobacterium leprae/classification , Mycobacterium leprae/genetics , Polymorphism, Single Nucleotide , Adolescent , Adult , Aged , Child , Child, Preschool , DNA, Bacterial , Family , Female , Humans , Leprosy/epidemiology , Male , Middle Aged , Molecular Typing , Mycobacterium leprae/isolation & purification , Young Adult
3.
Indian J Lepr ; 81(3): 119-24, 2009.
Article in English | MEDLINE | ID: mdl-20509339

ABSTRACT

Mycobacterium leprae strains from Indian leprosy patients were analyzed using the six base tandem repeat, GACATC, in rpoT gene as genetic marker. DNA was extracted from slit-skin smears and nasal swabs of new untreated as well as treated leprosy patients living in different regions of India. PCR amplification of rpoT gene and sequencing of amplicons showed the presence of two genotype of M. leprae in this study, 73.4% having three copies (ancient Indian type) and 26.6% contain 4 copies (considered to be Japanese and Korean). These genotypes along with other short tandem repeats may help in studying the historical spread of disease and the strains of M. leprae disseminated by various human races that migrated to India from other places of Asia and European countries during our history.


Subject(s)
Bacterial Proteins/genetics , DNA, Bacterial/genetics , Leprosy/microbiology , Mycobacterium leprae/genetics , Sigma Factor/genetics , Amplified Fragment Length Polymorphism Analysis , Animals , Emigration and Immigration , Genes, Bacterial , Genotype , Humans , India/epidemiology , Leprosy/epidemiology , Mycobacterium leprae/isolation & purification , Population Dynamics , Sequence Analysis, DNA , Skin , Tandem Repeat Sequences
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