[Genotyping of varicella zoster virus strains isolated in Mongolia].

This study analyzed 50 varicella zoster virus (VZV) samples collected during 2004 to 2007 from patients with VZV infection, who were treated at the National Center of Communicable Diseases, Ulan-Bator, Mongolia. The method based on amplification of specific DNA fragments of the ORF21, ORF22, and ORF50 genes was used, followed by the sequencing and detection of the status of characteristic point mutations in these fragments. The results indicated that the collected samples belonged to genotypes J (62%), M1 (18%), E1 (12%), E2 (4%), and M2 (4%). Moreover, restriction endonuclease polymorphism in ORF 62 for the cleavage site Smal and Mspl, in ORF 38 and ORF 54 for the cleavage site Pstl and Bgll were analyzed. All the samples were Sma- Msp-. All samples with genotype E were Pst+ Bgl-; all samples with genotype M1 and M2 were Pst+ Bgl+. Out of 31 samples with genotype J, 29 and 2 were Pst+ Bgl+ and Pst+ Bgl+, respectively. The study could identify the genotypes of VZV circulating in Mongolia and confirmed the absence of mutations characteristic for the vaccine strain.

Comparison of Human and Avian influenza virus agglutinahility to 4 kinds of animal red blood cells / Шинэ санаа Шинэ нээлт

BACKGROUND Every year 3-5 million cases have reported during human influenza epidemics and Disease 300 000-500 000 of them die. People who aged over 65 and under 2 year counted as a 'School of BioMeJicine. Health Sciences high risk group to influenza. When mix red blood cells and influenza virus, it shows llemaglutination reaction, which is caused by binding of influenza virus surface molecule hemagglutinin with red blood cell (RBC) receptor with syalic acid residues. This reaction is used traditionally as main and effective assay fw infl*wn*a virus detection. PURPOSE • To test local strains of A(H1N1), A(H3N2), A(II5N1) and B type influenza virus with human, guinea pig, chicken and horse RBCs • To choose the most sensitive animal RBC for detection of different influenza viruses METHODS We have used isolates of A/Ulaanbaatar/1 N85/07 (H,N,), /J| i laanbaatar 1654 II (H,N,), B/Ulaanbaalar/1877/07 isolated in the Respiratory Virology Laboratory, National Center for Communicable Diseases, A/Wooper Swan Mongolia 65 <16 (H5\ I) isolated in Central Veterinary Laboratory with human 01. horse, chicken and guinea pig RBCs. The subtyping of the Human influenza viruses have been performed with rt-PCR with specific primers. We used protocol of CDC, USA to isolate human influenza strains in MIX K cell culture, to determine strains with Hemagglutination test. RESULTS Human Influenza A(H IN I ).A(H3N2)and B type viruses are more agglutinable with to guinea pig RBCs Avian influenza A(H5N1) virus is more agglutinable with human 01 and horse RBC. CONCLUSIONS Guinea pig RBCs is the most effective for daily human influenza virus detection procedures and using horse and guinea pig RBC together is reasonable tor avian influenza suspected cases.