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1.
Vet World ; 14(10): 2678-2685, 2021 Oct.
Article in English | MEDLINE | ID: mdl-34903925

ABSTRACT

BACKGROUND AND AIM: The transition period is extremely critical for pregnant producing animals. However, there is very limited research on the metabolic and immunological changes in Egyptian water buffalo cows during the transition period. Therefore, this study was conducted to investigate the immunometabolic changes occurring during the transition period in Egyptian water buffalo cows. MATERIALS AND METHODS: A total of 50 multiparous pregnant Egyptian water buffalo cows were subjected to weekly blood sampling 3 weeks before calving and 3 weeks after calving and on the day of parturition to determine the complete blood count, including red blood cell count, total leukocyte count (TLC), differential leukocyte count, hemoglobin level, and packed cell volume (PCV). Some selected serum biochemical and immunological parameters were analyzed, including serum glucose, beta-hydroxybutyric acid (BHBA), non-esterified fatty acids, triglycerides, high-density lipoprotein, low-density lipoprotein (LDL), very LDL (VLDL), cholesterol, total protein, albumin, globulin, creatinine, blood urea nitrogen (BUN), aspartate aminotransferase, alkaline phosphatase, alanine transaminase, gamma-glutamyl transferase, Haptoglobin, and C-reactive protein and the pro-inflammatory cytokines interleukin ß1, interleukin 6 (IL-6), and tumor necrosis factor-alpha. All data were statistically analyzed using the IBM Statistical Package for the Social Sciences statistics software. RESULTS: The neutrophil count showed a statistically significant increase at 2 weeks preparturition. There was also a significant increase in PCV, TLC, neutrophil count, and IL-6 and TNF-a level at the time of parturition and even at 2 weeks post parturition, except PCV that returned to normal levels in the 1st week post parturition. BHBA and BUN levels were increased significantly in the 2nd and 3rd weeks postcalving. Serum creatinine and VLDL levels were decreased significantly at the time of parturition, and VLDL levels showed a significant decrease even till the 3rd week postcalving, whereas creatinine levels gradually returned to the pre-calving levels in the 3rd week postcalving. Other parameters showed no significant changes. CONCLUSION: The most important immunometabolic changes occur in the first 2 weeks post parturition in Egyptian water buffalo cows, which exhibit a potent, remarkable physiological adaptation achieved by their functional liver, which can help the animal overcome the stressful conditions during the transition period.

2.
Anim Reprod Sci ; 119(1-2): 17-23, 2010 May.
Article in English | MEDLINE | ID: mdl-20056360

ABSTRACT

The aim of the present study was to evaluate the efficacy of an Ovsynch protocol in the cyclic and non-cyclic Egyptian buffaloes in the summer. The present study was carried out on 21 Egyptian pluriparous buffalo cows (11 cyclic and 10 acyclic) and 8 heifers (5 cyclic and 3 acyclic). All animals were administered 100 microg GnRH i.m. at Day 0, 500 microg of PGF2 proportional, variant i.m. at Day 7 and a second dose (100 microg) of GnRH i.m. at Day 9. Ovarian structures of the animals were recorded by means of transrectal ultrasonography (6-8 MHz linear-array transducer) daily from Day 0 (before treatment) to Day 9 (the second GnRH administration) and thereafter, at 12-h interval till ovulation or for a maximum of 48 h. After ultrasound examination, a blood sample was taken daily from each animal for progesterone analysis. The buffalo cows were inseminated at 16-20 h after 2nd GnRH administration. After the first administration of GnRH, 46% (5 of 11) of cyclic and 50% (5 of 10) of acyclic cows and 40% (2 of 5) of cyclic and 33% (1 of 3) of acyclic heifers responded by ovulation or luteinization of the follicles. The mean diameters (+/-S.E.M.) of the follicles in the responding cows and heifers were significantly higher than those in non-responding animals (9.7 +/- 0.4 mm vs. 6.7 +/- 0.6 mm, P < 0.0001; 11.1 +/- 1.5 mm vs. 7.3 +/- 0.7 mm, P < 0.05, respectively). After the second administration of GnRH, 81% (9 of 11) of cyclic and 60% (6 of 10) of acyclic cows and 80% (4 of 5) of cyclic and 33% (1 of 3) of acyclic heifers ovulated, respectively. Ovulations occurred earlier (P = 0.059) and over a wider range of time in the non-cyclic cows (26 +/- 4.8 h; range: 12-36 h) and in a heifer (12 h,) than in the cyclic cows (34.7 +/- 1.3 h; range 24-36 h) and heifers (33 +/- 3 h; range: 24-36 h). The conception rate was 18% (2 of 11) and 0% (0 of 10) in cyclic and non-cyclic cows, respectively. In conclusion, the Ovsynch protocol could be used effectively for synchronization of ovulation in cyclic cows and heifers in summer.


Subject(s)
Buffaloes/physiology , Estrous Cycle/physiology , Estrus Synchronization/methods , Fertility Agents, Female/administration & dosage , Seasons , Anestrus/blood , Anestrus/drug effects , Anestrus/physiology , Animals , Buffaloes/blood , Cattle , Clinical Protocols , Cloprostenol/administration & dosage , Drug Administration Schedule , Egypt , Estrous Cycle/blood , Estrous Cycle/drug effects , Female , Gonadotropin-Releasing Hormone/administration & dosage , Insemination, Artificial/veterinary , Pregnancy , Pregnancy Rate , Progesterone/blood , Treatment Outcome
3.
Reprod Fertil Dev ; 19(2): 351-5, 2007.
Article in English | MEDLINE | ID: mdl-17257521

ABSTRACT

The aim of the present study was to evaluate the effect of a pretreatment with insulin on the response of buffalo cows with inactive ovaries to gonadotrophin-releasing hormone agonist (GnRHa) treatment during hot summer months (July and August). Thirty-six Egyptian buffalo cows with inactive ovaries were randomly allocated into three groups: (1) group treated with GnRHa (G1, n = 16) in which each buffalo received an intramuscular injection of 250 mg of GnRHa (Day 0; gonadorelin, Fertagyl); (2) group treated with insulin before the GnRHa injection (G2, n = 8) in which each buffalo received a subcutaneous injection of biphasic insulin at a dose of 0.25 IU kg(-1) bodyweight once daily starting at Day -3 for 3 consecutive days, followed by an intramuscular injection of 250 mg of GnRHa on Day 0; and (3) the control group (G3, n = 12) in which each buffalo cow received an intramuscular injection of 2.5 mL of sterile saline on Day 0. The ovaries of all animals were examined by trans-rectal ultrasonography (5 MHz) on Days -7, -3 and 0 and continued thereafter at 4-day intervals until oestrus or the end of the experiment. On the same days that ultrasound examinations were performed blood samples were collected to measure the progesterone concentrations. Administration of insulin for 3 days before GnRHa injection (G2) significantly (P < 0.05) increased the diameter of the largest follicle from 6.85 +/- 0.64 to 12.4 +/- 0.88 mm. There was a significant (P < 0.01) increase in the oestrous induction rate in G2 compared with both G1 and G3 during the first 12 days after the treatment interval. It is concluded that pretreatment with insulin for 3 days before GnRHa injection increases the diameter of the dominant follicle and therefore the oestrous induction rate of acyclic buffaloes.


Subject(s)
Buffaloes/physiology , Gonadotropin-Releasing Hormone/agonists , Insulin/administration & dosage , Ovary/drug effects , Ovulation Induction/methods , Animals , Estrus , Female , Organ Size/drug effects , Ovarian Follicle/anatomy & histology , Ovary/diagnostic imaging , Progesterone/blood , Seasons , Ultrasonography
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