A review on integrated vermifiltration as a sustainable treatment method for wastewater.

To overcome the scarcity of fresh water, concerned authorities worldwide are bound to think about remediation and reuse of domestic and industrial effluents. The present review study on integrated vermifiltrationwith hydroponic system explains mechanism followed in system and presently the reutilization and remediation of domestic and industrial effluents. It explains the result of integrated vermifiltration and recognizes factors such as clogging, hydraulic loading rate or rain on bed, salinity, and sunlight affect the efficiency of system. The study also focuses on limitations associated with vermifiltration and also suggestions have been made for enhancing the sustainability and performance of existing practices. After literature review, integrated vermifiltration with hydroponic system considered as a natural and eco-friendly method for treating polluted water. Active zone of vermifilter remove organics, nitrate from nitrogen, total and dissolved phosphorus from wastewater. The vermifiltration and integrated vermifiltration with macrophyte able remove chemical oxygen demand (COD) in the range (53.7%-64.4%) and (75.5%-82.8%) respectively. The integrated system reduces land consumption and wastewater can be reutilized in cultivation.

Cancer stem cells and field cancerization of head and neck cancer - An update.

Oral cancer results due to multiple genetic alterations that transform the normal cells in the oral cavity into neoplastic cells. These genetic changes in a particular tumor field lead to a rapid expansion of preneoplastic daughter cells producing malignant phenotype but the malignancy results due to such genetic changes occurr over several years. The morphological changes in these transformed cells help in the diagnosis of malignancy. Thus, the early changes at the gene level are present in the population of daughter cells in the organ, which explains the concept of field cancerization. Cancer stem cells (CSCs) represent a group of cells that have the capacity of self-renewal and have the potential to differentiate into other types of tumor cells. This review explains the cellular and genetic basis of field cancerization and the role of cancer stem cells in field cancerization.

Association of specific microorganisms with endodontic signs and symptoms. A comparative study.

AIM: The study aimed to evaluate the association of root canal microorganisms red complex and E. facealis with endodontic clinical signs and symptoms using polymerase chain reaction. MATERIALS AND METHODS: Bacterial samples were obtained using sterile paper points from the teeth of 100 subjects divided into two groups; Group I: 50 individuals with primary tooth infections and Group II: 50 individuals with failed endodontic treatment having the secondary infection. DNA extracted from samples was analyzed for endodontic pathogens by using species-specific primers. RESULTS: The pain was noticed in 66%, (33 of 50 subjects) in primary infection and 60% (30 of 50) in a secondary infection. A statistically significant association between pain and E. faecalis bacteria observed both in primary infection and secondary infection (P < 0.05). Tenderness on percussion was associated with 40% cases in Group I and 70% cases in Group II. The red complex accounted for 94% of cases associated with tenderness on percussion in primary infection while 86% of cases associated with secondary infection with a statistically significant association (P < 0.05). CONCLUSION: Prevalence of red complex bacteria and E. faecalis suggested the association of studied bacteria with symptomatic infected pulp and periradicular diseases.