Bacteria as biofactory of pigments: Evolution beyond therapeutics and biotechnological advancements.

Bacterial pigments are the wonder molecules of nature that have attracted the attention of industries in recent years. To date, various synthetic pigments have been in use in food, cosmetics, and textile industries that have not only shown a notoriously toxic nature but also posed threat to the ecosystem. Moreover, nutraceuticals, fisheries, and animal husbandry were highly dependent on plant sources for products that aid in disease prevention and improve stock health. In this context, the use of bacterial pigments as new-generation colorants, food fortifiers, and supplements can hold great prospects as low-cost, healthy, and eco-friendly alternatives. The majority of studies on these compounds were restricted to antimicrobial, antioxidant, and anticancer potentials to date. Each of these can be highly beneficial for the development of new-generation drugs, but their other potential niche in various industries that pose health and environmental risks needs to be explored. Recent advances in novel strategies of metabolic engineering, advancements in optimization tools for the fermentation process, and the design of appropriate delivery systems will greatly expand the market of bacterial pigments in industries. This review summarizes the current technologies for enhancing production, recovery, stability, and appreciable use of bacterial pigments in industries apart from therapeutics with proper financial aspects. The toxicity perspectives have been focused to emphasize that these wonder molecules are the need of the hour and their future prospects have been highlighted. Extensive literature has been studied to include the challenges of bacterial pigments from environmental and health risk perspectives.

Comparative performance evaluation of chitosan based polymeric microspheres and nanoparticles as delivery system for bacterial ß-carotene derived from Planococcus sp. TRC1.

ß-carotene is a natural compound with immense healthcare benefits. To overcome insolubility and lack of stability which restricts its application, in this study, ß-carotene from Planococcus sp. TRC1 was entrapped into formulations of chitosan­sodium alginate microspheres (MF1, MF2 and MF3) and chitosan nanoparticles (NF1, NF2 and NF3). The maximum entrapment efficiency (%) and loading capacity (%) were 80.6 ± 4.28 and 26 ± 3.05 (MF2) and 92.1 ± 3.44 and 41.86 ± 4.65 (NF2) respectively. Korsmeyer-Peppas model showed best fit with release, revealing non-Fickian diffusion. Thermal and UV treatment exhibited higher activation energy (kJ/mol), 17.76 and 15.57 (MF2) and 37.03 and 19.33 (NF2) compared to free ß-carotene (3.7 and 3.9), uncovering enhanced stability. MF2 and NF2 revealed swelling index (%) 721 ± 1.7 and 18.1 ± 1.5 (pH 6.8) and particle size 69.5 ± 3.2 µm and 92 ± 2.5 nm respectively. FESEM, FT-IR, XRD and DSC depicted spherical morphology, intactness of functional groups and masking of crystallinity. The IC50 (µg ml-1) values for antioxidant and anticancer (A-549) activities were 33.1 ± 1.7, 45.1 ± 2.8, 39.3 ± 2.9 and 31.3 ± 1.7, 27.9 ± 2.4, 25.3 ± 2.2 for ß-carotene, MF2 and NF2 respectively with no significant cytotoxicity on HEK-293 cells and RBCs (p > 0.05). This comparative study of microspheres and nanoparticles may allow the diverse applications of an unconventional bacterial ß-carotene with promising stability and efficacies.

Designing polymeric microparticulate drug delivery system for hydrophobic drug quercetin.

The aim of this study was to investigate pharmaceutical potentialities of a polymeric microparticulate drug delivery system for modulating the drug profile of poorly water-soluble quercetin. In this research work two cost effective polymers sodium alginate and chitosan were used for entrapping the model drug quercetin through ionic cross linking method. In vitro drug release, swelling index, drug entrapment efficiency, Fourier Transforms Infrared Spectroscopy (FTIR), Scanning Electron Microscopy (SEM), X-ray Diffraction (XRD) and Differential Scanning Calorimetric (DSC) studies were also done for physicochemical characterization of the formulations. Swelling index and drug release study were done at a pH of 1.2, 6.8 and 7.4 to evaluate the GI mimetic action which entails that the swelling and release of the all the Formulation1 (F1), Formulation2 (F2) and Formulation3 (F3) at pH 1.2 were minimal confirming the prevention of drug release in the acidic environment of stomach. Comparatively more sustained release was seen from the formulations F2 & F3 at pH 6.8 and pH 7.4 after 7 h of drug release profiling. Drug entrapment efficiency of the formulations shows in F1 (D:C:A = 2:5:30) was approximately 70% whereas the increase in chitosan concentration in F2 (D:C:A = 2:10:30) has shown an entrapment efficiency of 81%. But the comparative further increase of chitosan concentration in F3 (D:C:A = 2:15:30) has shown a entrapment of 80% which is not having any remarkable difference from F2. The FTIR analysis of drug, polymers and the formulations indicated the compatibility of the drug with the polymers. The smoothness of microspheres in F2 & F3 was confirmed by Scanning Electron Microscopy (SEM). However F1 microsphere has shown more irregular shape comparatively. The DSC studies indicated the absence of drug-polymer interaction in the microspheres. Our XRD studies have revealed that when pure drug exhibits crystalline structure with less dissolution profile, formulated microparticles can help us to obtain amorphous form of the same drug that is likely to have more dissolution property. The findings of the study suggest that the microsphere formulations were a promising carrier for quercetin delivery and can be considered as a favorable oral controlled release dosage form for hydrophobic drug quercetin.