Biocompatible polymer-coated magneto-fluorescent super nanoparticles for the homing of mesenchymal stem cells.

Stem cell plays an important role in the clinical field. However, the effective delivery of stem cells to the targeted site relies on the efficient homing of the cells to the site of injury. In view of that, fluorescent magnetic nanoparticles stick out due to their wide range of enabling functions including cellular homing and tracking. The present study unravels the synthesis of polymer-coated biocompatible and fluorescent magnetic nanoparticles (FMNPs) by a single-step hydrothermal synthesis method. Importantly, the facile method developed the biological super nanoparticles consisting of the magnetic core, which is surrounded by the fluorescent nanodot-decorated polymeric shell. The synthesized particles showed an amorphous nature, and superparamagnetic properties, with efficient fluorescence properties of emission at the blue range (Ì´ 410 nm). The FMNP labeling showed the mesenchymal stem cell (MSC) homing to the desired site in the presence of an external magnetic field. The in-house synthesized nanoparticles showed significant cytocompatibility and hemocompatibility in vitro as well as in vivo conditions owing to their surface coating. This unprecedented work advances the efficient internalization of FMNPs in MSCs and their enhanced migration potential provides a breakthrough in stem cell delivery for therapeutic applications. STATEMENT OF SIGNIFICANCE: The bi-modal fluorescent magnetic nanoparticles hold a promising role in the biomedical field for mesenchymal stem cell homing and tracking. Hence, in this study, for the first time, we have synthesized the fluorescent magnetic nanoparticle with polymer coating via an easy single-step method. The nanoparticle with a polymer coat enhanced the biocompatibility and effortless internalization of the nanoparticle into mesenchymal stem cells without hampering the native stem cell properties. Furthermore, the enhanced migration potential of such magnetized stem cells and their homing at the target site by applying an external magnetic field opened up avenues for the smart delivery of mesenchymal stem cells at complex sites such as retina for the tissue regeneration.

New isoniazid derivatives with improved pharmaco-toxicological profile: Obtaining, characterization and biological evaluation.

Tuberculostatic drugs are the most common drug groups with global hepatotoxicity. Awareness of potentially severe hepatotoxic reactions is vital, as hepatic impairment can be a devastating and often fatal condition. The treatment problems that may arise, within this class of medicines, are mainly of two types: adverse reactions (collateral, toxic or hypersensitive reactions) and the initial or acquired resistance of Mycobacterium tuberculosis to one or more antituberculosis drugs. Prevention of adverse reactions, increase treatment adherence and success rates, providing better control of tuberculosis (TB). In this regard, obtaining new drugs with low toxicity and high tuberculostatic potential is essential. Thus, in this work, we have designed or synthesized new derivatives of isoniazid (INH), such as new Isonicotinoylhydrazone (INH-a, INH-b and INH-c). These derivatives demonstrated good biocompatibility, antimicrobial property similar to that of parent isoniazid and last but not least, a significantly improved Pharmacotoxicological profile compared to that of isoniazid.

Species composition of microzooplankton Tintinnid from the coastal waters of Digha, Bay of Bengal.

Tintinnid species distribution and hydrography were studied in the coastal waters of Digha during winter (November 2015) and summer (March 2016) seasons. Surface water samples were collected from 11 different stations from 0 to 10 km offshore with the help of a mechanized trawler. Parameters like tintinnid species enumeration, zooplankton biomass, phytoplankton concentration (total chlorophyll) and abundance, sea surface temperature (SST), pH, transparency, salinity, dissolved oxygen (DO), total phosphate, silicate and nitrate were analysed. A total of 20 different tintinnid species (16 agglomerated +4 non-agglomerated) belonging to 6 genera were recorded from the study area with seasonal variation in tintinnid diversity, i.e. higher in summer (total 2745 individual/l) compared to winter (total 1191 individual/l). Tintinnopsis was the most dominant genus during both the seasons, i.e. 2100 individual/l in summer and 727 individual/l in winter, contributing about 76 and 61% population for the respective seasons. The correlation between species and water quality parameters showed that Tintinnopsis sp. abundance was significantly regulated by nitrate concentration, salinity, dissolved oxygen, water transparency and pH. However, the mentioned hydrological parameters were not the only factors regulating the tintinnid abundance. Tintinnid abundance was also found to be positively related with transparency (r = 0.732) and salinity (r = 0.524) and moderately related with dissolved oxygen (r = 0.488) whereas strong negative relation (at p ≤ 0.05) was established between tintinnid abundance with nitrate (r = -0.681) and pH (r = -0.561). Bray-Curtis cluster analysis of tintinnid species showed more than 60% similarity. Shannon's diversity index (H'), Simpson's evenness index (D) and Margalef's species richness index were found to be higher in summer, i.e. 1.61, 0.729 and 1.612, compared to the winter season, i.e. 1.139, 0.597 and 1.268. k-dominance curve showed maximum abundance of Tintinnopsis baltica in winter and Tintinnopsis gracilis in summer. Principal component analysis (PCA) was analysed to find out the environmental variables affecting different tintinnid species diversity. A significant spatiotemporal variation in Tintinnid population distribution was observed from two-way ANOVA. The results reflect significant seasonal (F = 840.0), spatial (F = 47.3) and interactive variation (F = 71.2) among the ciliate microzooplankton at n = 66, p ≤ 0.001. High chlorophyll content and phytoplankton population in summer indicated that tintinnid diversity in the season was positively influenced by producer community in coastal waters of Digha.