ABSTRACT
AIMS: To isolate plant growth-promoting bacterium from Western Ghat forests in India. METHODS AND RESULTS: A Gram-negative, rod shaped, cream white coloured strain Pantoea NII-186 isolated from Western Ghat soil sample. The taxonomic position of the bacterium was confirmed by sequencing of 16S rRNA and phylogenetic analysis. A strain grew at a wide range of temperature ranging from 5-40 degrees C, but optimum growth was observed at 28-30 degrees C. It showed multiple plant growth-promoting attributes such as phosphate solubilization activity, indole acetic acid (IAA) production, siderophore production and HCN production. It was able to solubilize (28 microg of Ca(3)PO(4) ml(-1) day(-1)), and produce IAA (59 microg) at 28 degrees C. The solubilization of insoluble phosphate was associates with a drop in the pH of the culture medium. Pantoea sp. NII-186 tolerate to different environmental stresses like 5-40 degrees C, 0-7% salt concentration and 4-12 pH range. CONCLUSIONS: The 16S rRNA gene sequence confirmed that the isolate NII-186 was belongs to Pantoea genus and showed considerable differences in physiological properties with previously reported species of this genus. Isolate NII-186 possessed multiple attributes of plant growth-promoting activity. SIGNIFICANCE AND IMPACT OF THE STUDY: Hence in the context it is proposed that Pantoea sp. NII-186, could be deployed as an inoculant to attain the desired plant growth-promoting activity in agricultural environment.
Subject(s)
Pantoea/isolation & purification , Pantoea/physiology , Plant Growth Regulators/biosynthesis , Soil Microbiology , Anti-Bacterial Agents/pharmacology , Cluster Analysis , DNA, Bacterial/chemistry , DNA, Bacterial/genetics , DNA, Ribosomal/chemistry , DNA, Ribosomal/genetics , Hydrogen Cyanide/metabolism , Hydrogen-Ion Concentration , India , Molecular Sequence Data , Pantoea/classification , Pantoea/genetics , Phosphates/metabolism , Phylogeny , Plants , RNA, Ribosomal, 16S/genetics , Sequence Analysis, DNA , Temperature , TreesABSTRACT
Multiple proteases were produced and partially purified from an alkali-thermotolerant novel species of Streptomyces (i.e., Streptomyces gulbargensis DAS 131) after 48 h of growth at 45 degrees C. The enzyme preparation exhibited activity over a broad range of pH (4-12) and temperature (27-55 degrees C). Optimum activity was observed at a pH of 9.0 and a temperature of 45 degrees C. Starch and protease peptone was found to be a good source of carbon and nitrogen to enhance the enzyme activity. Two active zones in the range of 19 to 35 kDa were detected on sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE).
Subject(s)
Bacterial Proteins/isolation & purification , Bacterial Proteins/metabolism , Peptide Hydrolases/isolation & purification , Peptide Hydrolases/metabolism , Streptomyces/enzymology , Bacterial Proteins/chemistry , Electrophoresis, Polyacrylamide Gel , Enzyme Stability , Hydrogen-Ion Concentration , Peptide Hydrolases/chemistry , Proteome/analysis , Substrate Specificity , TemperatureABSTRACT
A halophilic, Gram-positive, spore-forming motile Bacillus-like strain YIM 012(T), was isolated from one of the hypersaline soil samples collected in Xin-jiang province, China. Its optimum growth occurred at 10-20% of NaCl concentration (w/v), pH 7.0-8.0. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain YIM 012(T) is a member of the genus of Alkalibacillus, which is well supported by its chemotaxonomic and molecular characteristics. Based on its phenotypic evidence and genotypic data, Alkalibacillus halophilus sp. nov. was proposed and strain YIM 012(T) (=DSM 17369(T)=KCTC 3990(T)) was assigned as the type strain of the novel species.
