ABSTRACT
Effects of the microwave radiation on the culture of Chlamydomonas actinochloris green flagellar alga in the stationary phase of growth are studied. After exposure to radiation at the maximum dose of 125 J/g, the cell functional state worsened but all the studied parameters were restored in 20 days and in the long run found to be even better than the control indices. The data are compared with the similar ones obtained earlier for the lag phase culture. The studied sample is found to be more resistant to the irradiation than the previous one.
Subject(s)
Chlamydomonas/growth & development , Infrared Rays , Microwaves , Cell Proliferation/radiation effects , Chlamydomonas/radiation effects , Radiation DosageABSTRACT
The fluorescence spectra of the porous silicon modified by water solutions of biologically active materials and materials of biological origin are recorded as well as the fluorescence spectra of the porous silicon modified by lecithin monolayers grown on the surface of water solutions of the biologically active materials. The analysis of the obtained spectra made it possible to conclude on the effect of the studied materials on the content of ROS.
Subject(s)
Biological Products/chemistry , Lecithins/chemistry , Reactive Oxygen Species/analysis , Silicon/chemistry , Diphenhydramine/chemistry , Electrochemical Techniques , Fluorescence , Isoproterenol/chemistry , Porosity , Solutions , Spectrometry, Fluorescence , Surface Properties , Water/chemistryABSTRACT
Effect of the microwave irradiation on the subsequent development of the Chlamydomonas actinochloris culture is studied. The number of cells in the suspension was controlled and photoluminescence measurements were performed for 25 days to estimate the functional state of the cells. The exposure at a dose of 80 J/g is shown to negligibly affect the green alga, whereas the 122 J/g dose led to deterioration of the functional state and, thereafter, to the death of most cells. However, the survivors intensively developed, the culture restored the normal state for 20 days, reached and later even left behind the control sample in development.
Subject(s)
Chlamydomonas , Microwaves/adverse effects , Radiation Monitoring/methods , Cell Culture Techniques , Chlamydomonas/cytology , Chlamydomonas/growth & development , Chlamydomonas/radiation effects , Dose-Response Relationship, Radiation , Plant Cells/radiation effects , Time FactorsABSTRACT
The influence of microwave emission on the Vallisneria spiralis L. photoluminescence (PL) is studied. It is found that the irradiation leads to degradation of all the luminescence spectrum bands. It is shown that the influence on the chlorophyll PL at 715-735 and 800 nm is not limited by accompanied temperature effect. The degradation of PL has a temperature threshold at the thermal treatment. The 725 nm luminescence band of chlorophyll is shifted to short waves at both the irradiation and thermal treatment. The shift dependence from the irradiation dose and temperature is non-monotonous.