1.
J Immunol Methods
; 275(1-2): 113-21, 2003 Apr 01.
Article
in English
| MEDLINE
| ID: mdl-12667675
ABSTRACT
In this manuscript, we describe modifications to a commercial three-laser benchtop flow cytometer, as well as relevant biological methods, that allow analysis of up to five immunofluorescent parameters together with an ultraviolet (UV)-excitable DNA stain. This method allows expanded capacity for multiparameter immunophenotyping of complex mixed cell populations, together with accurate measurements of DNA content (cell cycle) or cell viability, on a stable, end-user operated platform.