Subject(s)
Aphakia/physiopathology , Lenses, Intraocular , Optometry/methods , Aphakia/surgery , Cornea , Darkness , Humans , Light , Refraction, OcularABSTRACT
Image analysis and Fourier transformation of the various nuclear immunofluorescence patterns observed while detecting antinuclear antibodies allow an objective and quantitative definition of the fluorescence. They also point out various IF types hidden by the main pattern, without having to dilute the test serum. They make obvious the difference between speckled and reticular patterns, and reveal the existence of intermediate states. The usual nuclear IF patterns (homogeneous, ring, nucleolar, reticulated, speckled and diffuse) may be grouped, according to their photo emission, into nuclear and subnuclear patterns. The first group includes homogeneous, annular and passive nucleolar IF. The second group is composed of speckled, reticulated, mixed, and active nucleolar IF. Alternatively, these aspects may be grouped into three types: homogeneous nuclear IF (homogeneous and ring), heterogeneous nuclear IF (speckled, reticulated and mixed) and nucleolar IF (active or passive). Diffusion can affect or not these aspects and does not apply to a special type or pattern. Image analysis and the study of the image spatial spectrum lead to automated recognition of the IF types, and later on, to the discrimination of antinuclear antibodies.
Subject(s)
Antibodies, Antinuclear/analysis , Cell Nucleus/immunology , Liver/immunology , Animals , Fluorescent Antibody Technique , Fourier Analysis , Histocytochemistry , Humans , Liver/ultrastructure , Rats , Spectrometry, FluorescenceABSTRACT
The ceramide trihexoside inclusions, which characterize Fabry-Anderson disease, have been studied using electron microscopy and digital image processing. Results are given showing the various apparent periodicities of the inclusion lamellae and the orientation, perpendicular to the lamellae or oblique, of the ceramide molecular component.
Subject(s)
Adipose Tissue/ultrastructure , Fabry Disease/metabolism , Glycosphingolipids , Muscles/ultrastructure , Synovial Fluid/ultrastructure , Trihexosylceramides , Adolescent , Adult , Aged , Chemical Phenomena , Chemistry , Child , Crystallography , Fabry Disease/genetics , Female , Fourier Analysis , Humans , Image Enhancement , Inclusion Bodies/ultrastructure , Male , Microscopy, Electron , Middle AgedABSTRACT
This paper deals with the various crystalline aspects of the ceramide trihexoside (CTH) inclusions, in the synovial tissue essentially. A basic classification is proposed and uncommon examples are illustrated. Image processing confirms the membrane-like structure of CTH inclusions and corroborates the proposed classification.
Subject(s)
Fabry Disease/pathology , Glycosphingolipids , Inclusion Bodies/ultrastructure , Trihexosylceramides , Adolescent , Adult , Aged , Child , Female , Humans , Inclusion Bodies/classification , Male , Microscopy, Electron , Middle AgedABSTRACT
The various patterns observed by generating Fourier transforms of the fixed normal corneal stroma are described. The patterns are analysed in terms of the dimensions of the stromal fibers, the dimensional and directional order of the fiber distribution and the order of the fiber-fiber functions by proteoglycans.
Subject(s)
Cornea/ultrastructure , Collagen/analysis , Fourier Analysis , Humans , Proteoglycans/analysisABSTRACT
An application of the Fourier Transform Process to the Fabry inclusion study has been made. The study, which rests upon five tissues and eight persons, brings out a new low frequency stratification. Various degrees of freedom exist for the basic molecule association. The periods are given for the three fundamental directions of the inclusion.