Biomining Sesuvium portulacastrum for halotolerant PGPR and endophytes for promotion of salt tolerance in Vigna mungo L.

Halophytic plants can tolerate a high level of salinity through several morphological and physiological adaptations along with the presence of salt tolerant rhizo-microbiome. These microbes release phytohormones which aid in alleviating salinity stress and improve nutrient availability. The isolation and identification of such halophilic PGPRs can be useful in developing bio-inoculants for improving the salt tolerance and productivity of non-halophytic plants under saline conditions. In this study, salt-tolerant bacteria with multiple plant growth promoting characteristics were isolated from the rhizosphere of a predominant halophyte, Sesuvium portulacastrum grown in the coastal and paper mill effluent irrigated soils. Among the isolates, nine halotolerant rhizobacterial strains that were able to grow profusely at a salinity level of 5% NaCl were screened. These isolates were found to have multiple plant growth promoting (PGP) traits, especially 1-aminocyclopropane-1-carboxylic acid deaminase activity (0.32-1.18 µM of α-ketobutyrate released mg-1 of protein h-1) and indole acetic acid (9.4-22.8 µg mL-1). The halotolerant PGPR inoculation had the potential to improve salt tolerance in Vigna mungo L. which was reflected in significantly (p < 0.05) higher germination percentage (89%) compared to un-inoculated seeds (65%) under 2% NaCl. Similarly, shoot length (8.9-14.6 cm) and vigor index (792-1785) were also higher in inoculated seeds. The strains compatible with each other were used for the preparation of two bioformulations and these microbial consortia were tested for their efficacy in salt stress alleviation of Vigna mungo L. under pot study. The inoculation improved the photosynthetic rate (12%), chlorophyll content (22%), shoot length (5.7%) and grain yield (33%) in Vigna mungo L. The enzymatic activity of catalase and superoxide dismutase were found to be lower (7.0 and 1.5%, respectively) in inoculated plants. These results revealed that halotolerant PGPR isolated from S. portulacastrum can be a cost-effective and ecologically sustainable method to improve crop productivity under high saline conditions.

Phytolith-Occluded Carbon Sequestration Potential of Oil Palm Plantation in Tamil Nadu.

Oil palm (Elaeis guineensis) has proven to be a phytolith-occluded carbon (PhytOC)-rich species that plays a vital role in acting as a carbon sink for reducing atmospheric carbon dioxide (CO2) concentration. The present research estimated the silicon, phytolith, and PhytOC contents in four (OP4), eight (OP8), and fifteen (OP15)-year-old oil palm plantations. Qualitative analysis using a scanning electron microscope (SEM) revealed the presence of abundant globular echinate phytoliths with varied diameter (8.484-10.18 µm) in fronds, empty fruit bunches, and roots. Furthermore, a wide band (400-490 cm-1) underlined a higher relative abundance of Si-OH groups in empty fruit bunches, fronds, and roots, which emphasized the amorphous nature of silica. Quantitative analysis revealed that the phytolith (phytolith/dry biomass), PhytOC (PhytOC/phytolith), and PhytOC (PhytOC/dry biomass) contents in all oil palms differed significantly (p < 0.05) and increased with age. The PhytOC stock showed significant variation, with the trend of OP15 > OP8 > OP4. The belowground biomass of OP4 (16.43 g kg-1) and OP8 (17.13 g kg-1) had a maximum PhytOC concentration compared to the aboveground biomass, and the belowground proportion varied from 20.62 to 20.65%. The study demonstrated a positive correlation between the phytolith and PhytOC contents of oil palm; thereby, oil palm should be cultivated for enhanced long-term sequestration as a phytolith accumulator.

Mitigation of nitrous oxide emission through fertigation and 'N' inhibitors - A sustainable climatic crop cultivation in tomato.

The impact of nitrous oxide (N2O) released from the fertilized agro-ecosystems are of increasing concern. Governing fertilizer requirements and utilizing nitrification inhibitors (NI) are effective methodologies to increase nitrogen retention and reduce N2O emissions from soil. Therefore, the effect of potassium thiosulfate (KTS) and neem-coated urea (NCU) on N2O efflux under irrigated tomato cultivation was assessed. Soil Test Crop Response (STCR) based recommendation of NPK with normal Urea and KTS at 1% of applied N (183:160:125 kg ha-1) (STCR-U + KTS) recorded the least N2O emission and high efficiency in suppressing the nitrate reductase activity. STCR-NCU was on par with STCR-U + KTS, reporting a higher reduction of N2O (21.1, 31.2, and 34.4% during the basal application, 1st and 2nd top dressing, respectively) compared to the blanket recommendation of nutrients. Similarly, STCR-U + KTS recorded the highest reduction (26.2, 25.6, and 30.9% during the basal application, 1st and 2nd top dressing, respectively) after fertilizer application. Besides, the yield of tomatoes is increased in the STCR-NCU (14.08%) and STCR-U + KTS (12.48%) with good quality fruit along (AA, Lycopene, and TSS contents) with low N2O emissions. The DeNitrification-DeComposition (DNDC) model further revealed that the simulated data and assessed findings were in good accord, proving the model's reliability and use as a tool for predicting the efficiency of fertilizer application.

Mitigation of methane gas emissions in flooded paddy soil through the utilization of methanotrophs.

The utilization of methanotrophs for reducing the global warming potential in a flooded paddy system is the progressive investigation in the recent. The field investigation was conducted in Tamil Nadu Rice Research Institute, Aduthurai. This study showed the effect of isolated methanotroph strain (MR15) on the reduction of methane emission and improvement in growth parameters and yield of paddy. The treatments included the diverse dosages of methanotroph consortium (0, 25, 50, 75 & 100%). The total seasonal methane emission varied between 1.96 and 5.04 kg ha-1 for the season of Kharif and 2.18 to 5.81 kg ha-1 for the season of Rabi owing to the footprint of methanotroph. Irrespective of treatments, the mean seasonal emission was more prominent during Rabi compared to the Kharif season. The dosage of methanotroph consortium significantly influenced not only seasonal methane emission but also the grain yield percentage, which increased over 100% Recommended Dose of Fertilizers (RDF). Application of 100% methanotroph consortium (i.e.6.25 kg ha-1) minimizes the methane emission by 60% with an increase in grain yield by 35% and lessens the usage of additional nutrients. Overall, this study showed a sign of atmospheric methane uptake with increase growth potential and yield of paddy cultivation using methanotroph consortium.