Physicochemical mechanism of BSA, Hb, and dsDNA biomacromolecules with aq-NaCMC at 298.15-310.15 K interfaced with UV-vis fluorescence circular dichroism spectroscopy and in-silico for interacting activities.

The 0.2-0.8mg% @ 0.2 mg% bovine serum albumin (BSA 66), hemoglobin (Hb 64.5), and double stranded deoxyribonucleic acid (dsDNA 130 kDa) with water and 0.25, 0.50, and 1.00 g% aqueous carboxymethyl cellulose sodium salt (NaCMC) thermodynamically, kinetically, and tentropically stable homogeneous solutions via resonating energy transfer were designed. Density (ρ), viscosity (η), surface tension (γ), friccohesity (σ), Gibbs free energy (ΔGb), chemical potential (µ), frictional volume (ϕ), apparent molar volume (V2), hydrodynamic radius (Rh), and isentropic compressibility (ksϕ) at 298.15, 304.15, and 310.15 K were studied. UV-Vis spectrophotometry, fluorescence spectroscopy, circular dichroism (CD), MALDI TOF, and in-silico study via reorientational activities have elucidated structural recognition. The ρ, η, γ, σ, ΔGb, µ, V2, ϕ, Rh, and ksϕ physicochemical properties (PCPs) have studied the interacting activities of salt bridges (peptide bonds) of proteins and base pairs (adenine, thymine, cytosine, guanine) of dsDNA on developing nanohydration sphere (NHS) with water and 0.25, 0.50, and 1.00 g% aq-NaCMC. Regression constants of PCPs have elaborated the interacting mechanism of internal linkages of BSA, Hb, and dsDNA for solubilizing them without unfolding assisted by glycosidic bonds of glucopyranose units of NaCMC. Magnitudes of regression constants analyse the inner salt bridges, electrostatic dipoles, and hydrogen bonds (HB) to interact with H2O dipoles and NaCMC affecting solubilization of biomolecules (biomols) in areas of biochemical, biophysical, bioengineering, tissue, and genetic engineering. PCPs, UV-Vis, fluorescence, CD, and in-silico study analyse their structurally interacting abilities with NaCMC via respective NHS to be extended to pharmaceutical and cosmetic processes by minimizing quantum energy barrier (QEB) as Newtonian behaviour of structural sensors.

Study of molar properties of GO after doping with transition metals for photodegradation of fluorescent dyes.

We synthesized graphene oxide (GO) doped with transition metal ions and characterized it using XPS, FT-IR, TGA/DTG, XRD, SEM, AFM, ICP-OES, UV/vis, and Raman spectroscopy. An intrinsic viscosity [η] of 0.002-0.012 g% @ 0.002 aq-GO was determined for viscosity average molecular weight (M v) of GO at 288.15, 298.15, and 308.15 K. Mark-Houwink (M-H) constants k (cm3 g-1) and a (cm3 mol g-2) were calculated for 5-15 mg/100 mL polyvinylpyrrolidone (PVP), using 29, 40, 55 kg mol-1 as markers for calculating M v by fitting the [η] to the Mark-Houwink-Sakurada equation (MHSE). We obtained 48 134.19 g mol-1 M v at 298.15 K, and the apparent molar (V ϕ m , cm3 mol-1), limiting molar volumes (V 0 GO)GO⃑0, enthalpy (ΔH m, J mol-1), entropy (ΔS m, J mol-1 K-1), viscosity (η m, mPa s mol-1), surface tension (γ m, mN m-1 mol-1), friccohesity (σ m, scm-1 mol-1), fractional volume (ϕ m, cm3 mol-1), isentropic compressibility (K sϕ,m, 10-4 cm s2 g-1 mol), infer GO molar consistency throughout the chemical processes. Molar properties (MPs) infer a GO monodispersion producing negative electrons (e-) and positive holes (h+) under sunlight. The transition metal ions (Fe2+, Mn2+, Ni2+, Cr3+, TMI) doped onto GO (TMI-GO), can photodegrade methylene blue (MB) in 60 min compared with 120 min using GO alone. The 4011 C atoms, 688 hexagonal sheets, 222 π-conjugations, and 4011 FE were calculated from the 48 134.19 g mol-1. The functional edges are the negative and positive holes generating centres of the GO 2D sheets.