ABSTRACT
Mumps outbreaks have recently been recorded in a number of highly vaccinated populations. We related seroprevalence, epidemiological and vaccination data from 18 European countries participating in The European Sero-Epidemiology Network (ESEN) to their risk of mumps outbreaks in order to inform vaccination strategies. Samples from national population serum banks were collected, tested for mumps IgG antibodies and standardized for international comparisons. A comparative analysis between countries was undertaken using age-specific mumps seroprevalence data and information on reported mumps incidence, vaccine strains, vaccination programmes and vaccine coverage 5-12 years after sera collection. Mean geometric mumps antibody titres were lower in mumps outbreak countries [odds ratio (OR) 0·09, 95% confidence interval (CI) 0·01-0·71)]. MMR1 vaccine coverage ⩾95% remained protective in a multivariable model (P < 0·001), as did an interval of 4-8 years between doses (OR 0·08, 95% CI 0·01-0·85). Preventing outbreaks and controlling mumps probably requires several elements, including high-coverage vaccination programmes with MMR vaccine with 4-8 years between doses.
Subject(s)
Antibodies, Viral/blood , Disease Outbreaks , Mumps Vaccine , Mumps virus/immunology , Mumps/epidemiology , Mumps/immunology , Vaccination/statistics & numerical data , Adolescent , Adult , Child , Child, Preschool , Europe/epidemiology , Female , Humans , Immunization Schedule , Incidence , Infant , Male , Middle Aged , Seroepidemiologic Studies , Young AdultABSTRACT
Countries with no autochthonous measles run the risk of the virus being imported by travellers and transmitted to unprotected citizens. In April 2012, two travellers from Finland and one from Estonia were diagnosed with measles after returning from Phuket, Thailand. They were contagious on their return flights and subsequently exposed several individuals, prompting extensive infection control measures. Two secondary cases were detected: one child who had received one vaccine dose and another who was fully vaccinated.
Subject(s)
Aircraft , Communicable Disease Control/methods , Measles Vaccine/immunology , Measles virus/isolation & purification , Measles/prevention & control , Travel , Adolescent , Adult , Child , Communicable Disease Control/standards , Contact Tracing/methods , Cross Infection/virology , Disease Notification , Disease Outbreaks/prevention & control , Estonia/epidemiology , Female , Finland/epidemiology , Guidelines as Topic , Humans , Measles/diagnosis , Measles/epidemiology , Measles Vaccine/administration & dosage , Measles virus/immunology , Measles-Mumps-Rubella Vaccine/isolation & purification , Patient Admission , Risk Factors , Thailand/epidemiologyABSTRACT
The WHO recommends hepatitis A virus (HAV) immunization according to level of transmission and disease burden. We aimed to identify susceptible age groups by standardized serosurveys to inform HAV vaccination policy in participating countries: Belgium, Czech Republic, England, Finland, Germany, Italy, Lithuania, Malta, Romania, and Slovakia. Each country tested national serum banks (n = 1854-6748), collected during 1996-2004, for anti-HAV antibodies. Local laboratory results were standardized to common units. Forty-one per cent of those aged <30 years and 6% of those aged ≥30 years were susceptible to HAV in Romania; compared to 70-94% and 26-71%, respectively, elsewhere. Romania reported high HAV incidence in children and young adults. Other countries reported HAV disease primarily in older risk groups. The results suggest low level of HAV transmission in most of Europe. Romania, however, appeared as an area with intermediate transmission. Vaccination of risk groups in countries with high susceptibility of young and middle-aged adults needs to be continued.
Subject(s)
Hepatitis A Antibodies/blood , Hepatitis A Virus, Human/immunology , Hepatitis A/epidemiology , Adolescent , Adult , Age Factors , Child , Child, Preschool , Europe/epidemiology , Female , Health Policy , Hepatitis A/immunology , Hepatitis A/transmission , Humans , Incidence , Infant , Male , Middle Aged , Prevalence , Seroepidemiologic Studies , Young AdultABSTRACT
Since May 2009, the pandemic influenza A(H1N1) virus has been spreading throughout the world. Epidemiological data indicate that the elderly are underrepresented among the ill individuals. Approximately 1,000 serum specimens collected in Finland in 2004 and 2005 from individuals born between 1909 and 2005, were analysed by haemagglutination-inhibition test for the presence of antibodies against the 2009 pandemic influenza A(H1N1) and recently circulating seasonal influenza A viruses. Ninety-six per cent of individuals born between 1909 and 1919 had antibodies against the 2009 pandemic influenza virus, while in age groups born between 1920 and 1944, the prevalence varied from 77% to 14%. Most individuals born after 1944 lacked antibodies to the pandemic virus. In sequence comparisons the haemagglutinin (HA) gene of the 2009 pandemic influenza A(H1N1) virus was closely related to that of the Spanish influenza and 1976 swine influenza viruses. Based on the three-dimensional structure of the HA molecule, the antigenic epitopes of the pandemic virus HA are more closely related to those of the Spanish influenza HA than to those of recent seasonal influenza A(H1N1) viruses. Among the elderly, cross-reactive antibodies against the 2009 pandemic influenza virus, which likely originate from infections caused by the Spanish influenza virus and its immediate descendants, may provide protective immunity against the present pandemic virus.
Subject(s)
Cross Reactions/immunology , HIV Antibodies/immunology , Influenza A Virus, H1N1 Subtype/genetics , Influenza, Human/epidemiology , Adolescent , Adult , Aged , Aged, 80 and over , Amino Acid Sequence , Child , Child, Preschool , Female , Finland/epidemiology , HIV Antibodies/blood , Humans , Influenza A Virus, H1N1 Subtype/immunology , Influenza A Virus, H2N2 Subtype/immunology , Influenza, Human/diagnosis , Influenza, Human/virology , Male , Middle Aged , Young AdultABSTRACT
It has been suggested that the incidence of herpes zoster may increase due to lack of natural boosting under large-scale vaccination with the varicella vaccine. To study the possibility and magnitude of such negative consequences of mass vaccination, we built a mathematical model of varicella and zoster epidemiology in the Finnish population. The model was based on serological data on varicella infection, case-notification data on zoster, and new knowledge about close contacts relevant to transmission of infection. According to the analysis, a childhood programme against varicella will increase the incidence of zoster by one to more than two thirds in the next 50 years. This will be due to increase in case numbers in the 35 years age groups. However, high vaccine coverage and a two-dose programme will be very effective in stopping varicella transmission in the population.
Subject(s)
Chickenpox Vaccine/administration & dosage , Chickenpox Vaccine/immunology , Chickenpox/prevention & control , Herpes Zoster/prevention & control , Herpesvirus 3, Human/immunology , Vaccination/methods , Adolescent , Adult , Aged , Aged, 80 and over , Chickenpox/epidemiology , Child , Child, Preschool , Computer Simulation , Data Collection/methods , Female , Herpes Zoster/epidemiology , Humans , Infant , Infant, Newborn , Male , Middle Aged , Models, Theoretical , Young AdultABSTRACT
Little is known about the public health burden of rabies in rabies-free countries. In these countries, the surveillance of suspected and treated cases serves as a substitute for estimating the risk and burden of human rabies because deaths due to rabies are extremely rare. Suspected rabies exposures among Finnish inhabitants were characterized using data from the National Infectious Disease Registry as well as animal surveillance data from the Finnish Food Safety Authority Evira, 1995-2006. In total, 195 suspected rabies exposures were reported (incidence 3/million inhabitants/year). Exposures were equally common among both genders and the median age was 35 years. Exposures were more common among 20- to 49-year olds than among other age groups. Less than one-third of the exposures occurred in Finland (incidence of indigenous exposures 0.9/million inhabitants/year). Indigenous rabies exposures were most frequently reported in southeastern Finland, with cats and dogs as the main sources. The high prevalence in the Baltic countries and Russia poses a risk for rabies reintroduction. The present control of wildlife rabies appears successful and important. The import of animals from endemic areas, however, remains a risk, which can be reduced by increasing public awareness of the disease, vaccination of imported animals and better rabies control in endemic countries.
Subject(s)
Rabies Vaccines/immunology , Rabies/veterinary , Adolescent , Adult , Aged , Aged, 80 and over , Animals , Cats , Child , Child, Preschool , Dogs , Female , Finland/epidemiology , Humans , Immunoglobulins/immunology , Male , Middle Aged , Population Surveillance , Rabies/epidemiology , Rabies/prevention & control , Young Adult , ZoonosesABSTRACT
The European Sero-Epidemiology Network 2 (ESEN2) aimed to compare serological results of vaccine-preventable diseases across Europe. To ensure direct inter-country comparability of hepatitis A virus antibody (anti-HAV) measurements, a standardization panel of 150 sera was developed by a designated reference laboratory and tested by participating national laboratories using assays of choice; each country's results were subsequently regressed against those of the reference laboratory. Quantitatively, the assays were generally highly correlated (R2>0.90). Nevertheless, qualitative comparisons indicated that results obtained with different assays may differ despite the usage of well-established international and local standards. To a great extent standardization successfully alleviated such differences. The generated standardization equations will be used to convert national serological results into common units to enable direct international comparisons of HAV seroprevalence data. The results of this study are expected to contribute to the evaluation and potential improvement of the currently employed immunization strategies for hepatitis in Europe.
Subject(s)
Hepatitis A Antibodies/blood , Hepatitis A virus/immunology , Hepatitis A/diagnosis , Hepatitis A/epidemiology , Serologic Tests/standards , Europe/epidemiology , Hepatitis A virus/isolation & purification , Humans , Regression Analysis , Seroepidemiologic StudiesABSTRACT
To inform current and future vaccination strategies, we describe the seroepidemiology of hepatitis B virus (HBV) infection in ten representative European countries using standardized serology that allowed international comparisons. Between 1996 and 2003, national serum banks were compiled by collecting residual sera or by community sampling; sera were then tested by each country using its preferred enzyme immunoassays and testing algorithm, and assay results were standardized. Information on current and past HBV vaccination programmes in each country was also collected. Of the ten countries, six reported low levels (<3%) of antibodies against HBV core antigen (anti-HBc). Of the eight countries testing for HBV surface antigen (HBsAg), the highest prevalence was reported in Romania (5.6%) and in the remaining seven countries prevalence was <1%. Universal HBV vaccination programmes had been established in seven countries as recommended by the World Health Organization, but the seroprevalence of antibodies against HBsAg (anti-HBs) was lower than the reported vaccine coverage in three countries. Regular serological surveys to ascertain HBV status within a population, such as reported here, provide important data to assess the need for and to evaluate universal HBV vaccination programmes.
Subject(s)
Hepatitis B/epidemiology , Adolescent , Adult , Age Distribution , Child , Child, Preschool , Europe/epidemiology , Female , Hepatitis B/blood , Hepatitis B Antibodies/blood , Humans , Male , Middle Aged , Seroepidemiologic Studies , Young AdultABSTRACT
Investigation of hepatitis A (HA) outbreak developed in 2005 among workers of food stores networkwas performed using conventional epidemiologic diagnostics as well as methods of molecular epidemiology. In 14 of 15 ill persons, using polymerase chain reaction, HAV RNA was detected by PCR in serum obtained on 2 - 25 day of illness (mean - 9.3 days). In 10 cases it was possible to determine nucleotide sequence of VP1/VP2 region of HAV genome and perform phylogenetic analysis of obtained isolates. It was determined that all isolates belonged to subgenotype IA, had high degree of homology and grouped in one cluster. These findings demonstrate their descendance from one source of infection, which, with high degree of probability, was the cook who made salads from fresh vegetables. HAV strain, which caused this epidemic outbreak circulates in Saint Petersburg for a long time and was already detected in 2004. Importance of vaccination against HA for persons working in manufacturing and distribution of food and use of molecular epidemiologic methods of surveillance for this infection is underlined.
Subject(s)
Cooking and Eating Utensils , Disease Outbreaks , Hepatitis A Virus, Human/classification , Hepatitis A/epidemiology , Hepatitis A/transmission , Adolescent , Adult , Female , Hepatitis A/prevention & control , Hepatitis A/virology , Hepatitis A Virus, Human/genetics , Hepatitis A Virus, Human/isolation & purification , Humans , Male , Middle Aged , Molecular Epidemiology , Phylogeny , RNA, Viral/analysis , Russia/epidemiology , Urban Population , Viral Structural Proteins/genetics , Young AdultABSTRACT
We conducted a seroprevalence survey in Belgium, Finland, England & Wales, Italy and Poland on 13 449 serum samples broadly representative in terms of geography and age. Samples were tested for the presence of immunoglobulin G antibody using an enzyme immunoassay. The age-specific risk of infection was estimated using parametric and non-parametric statistical modelling. The age-specific risk in all five countries was highest in children aged 7-9 years and lower in adults. The average proportion of women of child-bearing age susceptible to parvovirus B19 infection and the risk of a pregnant women acquiring B19 infection during pregnancy was estimated to be 26% and 0.61% in Belgium, 38% and 0.69% in England & Wales, 43.5% and 1.24% in Finland, 39.9% and 0.92% in Italy and 36.8% and 1.58% in Poland, respectively. Our study indicates substantial epidemiological differences in Europe regarding parvovirus B19 infection.
Subject(s)
Parvoviridae Infections/epidemiology , Parvovirus B19, Human/immunology , Adolescent , Adult , Age Factors , Aged , Antibodies, Viral/blood , Belgium/epidemiology , Child , Child, Preschool , England/epidemiology , Female , Finland/epidemiology , Humans , Immunoglobulin G/blood , Infant , Italy/epidemiology , Male , Middle Aged , Poland/epidemiology , Pregnancy , Pregnancy Complications, Infectious/epidemiology , Prevalence , Risk Factors , Seroepidemiologic Studies , Sex Factors , Statistics, Nonparametric , Wales/epidemiologyABSTRACT
The European sero-epidemiology network (ESEN2) aims to standardise serological surveillance of varicella zoster virus (VZV) in 11 participant countries. In each country, serum banks were collected between 1996 and 2003 and tested for VZV antibodies. Assay results were standardised so that international comparisons could be made. Age-specific forces of infection were calculated for three age groups (<5, 5-9 and >or=10 years of age) and used to estimate the base reproduction number (R(0)) and the herd immunity threshold (H). Most VZV infection occurred in childhood, but there was a wide variation in transmissibility, with R(0) ranging from 16.9 in the Netherlands to 3.3 in Italy. Herd immunity thresholds varied from 70% in Italy to 94% in the Netherlands. There are substantial differences in VZV sero-epidemiology within the European region, which will need to be taken into account in designing national policies regarding VZV vaccination.
Subject(s)
Herpesvirus 3, Human/immunology , Immunization/statistics & numerical data , Seroepidemiologic Studies , Antibodies, Viral/analysis , Antigens, Viral/analysis , Europe/epidemiology , Herpes Zoster/epidemiology , Herpes Zoster/prevention & control , Humans , Italy , Netherlands , Vaccination , White PeopleABSTRACT
The aim of the European Sero-Epidemiology Network 2 was to coordinate and standardize the serological surveillance of vaccine-preventable diseases in Europe. In this study, the standardization of hepatitis B virus (HBV) results is described. The 15 participating national laboratories tested a unique panel of 172 sera established by the Greek reference centre for HBV surface antigen (HBsAg), antibodies to HBsAg (anti-HBs) and/or to the HBV core antigen (anti-HBc) by assay methods of their choice. Country-specific quantitative measurements for anti-HBs and anti-HBc were transformed into common units using standardization equations derived by regressing each country's panel results against the reference centre's results, thus adjusting for interassay and interlaboratory variability. For HBsAg, a qualitative analysis (positive/negative) showed at least 99% agreement with the reference laboratory for all countries. By combining these standardized and qualitative results for the markers mentioned earlier, it was possible to achieve comparable estimates of the proportion of the population susceptible to HBV, vaccinated against HBV, with a past HBV infection, and with a current infection or chronic carrier state. Standardization is a very important tool that allows for international serological comparisons to assess the current vaccination policies and the progress of HBV control in Europe.
Subject(s)
Hepatitis B Antibodies/blood , Hepatitis B Core Antigens/blood , Hepatitis B Surface Antigens/blood , Hepatitis B virus/immunology , Hepatitis B/virology , Europe/epidemiology , Hepatitis B/blood , Hepatitis B/epidemiology , Hepatitis B/prevention & control , Hepatitis B Vaccines/immunology , Hepatitis B Vaccines/therapeutic use , Humans , Reagent Kits, Diagnostic/standards , Seroepidemiologic Studies , Serologic Tests/standardsABSTRACT
The aim of the European Sero-Epidemiology Network is to establish comparability of the serological surveillance of vaccine-preventable diseases in Europe. The designated reference laboratory (RL) for measles, mumps, rubella (MMR) prepared and tested a panel of 151 sera by the reference enzyme immunoassay (rEIA). Laboratories in 21 countries tested the panel for antibodies against MMR using their usual assay (a total of 16 different EIAs) and the results were plotted against the reference results in order to obtain equations for the standardization of national serum surveys. The RL also tested the panel by the plaque neutralization test (PNT). Large differences in qualitative results were found compared to the RL. Well-fitting standardization equations with R2> or =0.8 were obtained for almost all laboratories through regression of the quantitative results against those of the RL. When compared to PNT, the rEIA had a sensitivity of 95.3%, 92.8% and 100% and a specificity of 100%, 87.1% and 92.8% for measles, mumps and rubella, respectively. The need for standardization was highlighted by substantial inter-country differences. Standardization was successful and the selected standardization equations allowed the conversion of local serological results into common units and enabled direct comparison of seroprevalence data of the participating countries.
Subject(s)
Antibodies, Viral/blood , Immunoenzyme Techniques/standards , Measles/epidemiology , Mumps/epidemiology , Rubella/epidemiology , Australia/epidemiology , Europe/epidemiology , Humans , Seroepidemiologic StudiesABSTRACT
The morbidity structure was analyzed in children vaccinated against epidemic parotitis in 1993-2002. Eight children (4 with serous meningitis and 4 with lesions of the salivary glands) underwent virologic and immunologic examinations. The molecular typing of the SH-gene fragment of the parotitis virus showed the process in 7 cases to be provoked by the vaccination strain. Presumedly, progressing vaccine-associated meningitis inhibits antibody formation. The total incidence of vaccine-associated meningitis was shown, according to Saint Petersburg data, to be not high, which testifies to a low reactogenicity of the Russian vaccine strain.
Subject(s)
Meningitis/etiology , Mumps Vaccine/adverse effects , Mumps/etiology , Mumps/prevention & control , Rubulavirus , Vaccination/adverse effects , Adolescent , Antibodies, Viral/blood , Antigens, Viral/analysis , Child , Child, Preschool , Humans , Incidence , Infant , Meningitis/blood , Mumps/epidemiology , Phylogeny , Retrospective Studies , Rubulavirus/genetics , Rubulavirus/immunology , Rubulavirus/isolation & purification , Russia/epidemiology , Salivary Glands/pathology , Salivary Glands/virology , Urban Population , Viral Proteins/geneticsABSTRACT
Before rubella vaccination programmes began, rubella infection was prevalent in Finnish children. The disease occurred as epidemics at intervals of a few years. Rubella infection was most often contracted between the ages of 2 and 12 years. Vaccinations specifically aimed at eradicating rubella were begun with monocomponent vaccine in the mid-1970s, and the measles, mumps and rubella (MMR) vaccination programme with two injections got underway in 1982. A clear reduction in rubella cases was evident a few years after the launch of the MMR programme. Owing to a sufficiently high vaccination coverage (>95% since 1987), circulation of the indigenous rubella virus in the Finnish population ceased in the late 1990s. Some rubella cases have been imported to Finland since elimination, but they have not caused any secondary cases. This shows unambiguously that protection against rubella continues to be effective, although our cohort studies imply that the vaccine induced antibody levels do decrease with time. The MMR programme has also eliminated congenital rubella syndrome (CRS) from the country. The last CRS case was recorded in 1986. As a result of the high coverage two dose MMR vaccination programme, rubella was successfully eliminated from Finland. How long the acquired protection will last remains to be seen.
Subject(s)
Rubella/epidemiology , Adolescent , Adult , Child , Female , Finland/epidemiology , Humans , MaleABSTRACT
A prolonged outbreak of hepatitis A infection amongst drug users in Suffolk prompted a study of the natural immunity against hepatitis A in this population, and a retrospective analysis of the relationship between specific drug-taking behaviours and the risk of hepatitis A infection. Prior to the outbreak, age-specific seroprevalence of hepatitis A IgG in drug users was similar to that amongst blood donors in the region. Of those without effective immunity, intravenous drug users, multiple drug users and those injecting frequently were more likely to have developed hepatitis. The reported frequency of equipment sharing and the number of injecting partners were not related to the risk of infection. The potential for blood-to-blood, and a suggested faecal-blood transmission were considered to be important in propagating the outbreak in this population. We suggest that a single dose of hepatitis A vaccine administered opportunistically should be used in outbreaks involving drug users.
Subject(s)
Disease Outbreaks , Disease Transmission, Infectious , Hepatitis A/epidemiology , Hepatitis A/transmission , Substance Abuse, Intravenous , Adult , Case-Control Studies , England/epidemiology , Female , Hepatitis A/etiology , Hepatitis A/prevention & control , Hepatitis A Vaccines/therapeutic use , Hepatitis A Virus, Human/immunology , Humans , Male , Risk Factors , Risk-TakingABSTRACT
Seroepidemiological study of hepatitis A (HA) morbidity was carried out in three Russian cities, with different levels of HA morbidity. The study included the analysis of HA morbidity for 22 years, the determination of antibodies to HA virus (anti-HAV) in 2,958 healthy persons aged 0-12 months to 40 years and older. In one of the cities 7 isolates of HA virus were obtained from unrelated sources and the genotypes of the virus were determined. The study revealed that the frequency of seropositive cases among persons of different ages correlated with the level and prolonged dynamics of HA morbidity. According to the occurrence of anti-HAV, such cities as St. Petersburg, Rostov-on-Don and Yakutsk may be at present classified as territories, moderately endemic in HA. At the same time in the 90 s the epidemic situation in HA was more favorable in Rostov-on-Don than in two other cities. The suggestion was made that a high proportion of seropositive persons among the population of St. Perersburg was linked with an almost twofold rise in HA morbidity in 1993-1995 caused by genotype 1 of the virus. Seroepidemiological studies in HA during the period of a drop in morbidity acquire special importance in the surveillance and control system of this infection.
Subject(s)
Disease Outbreaks , Hepatitis A/epidemiology , Hepatovirus/isolation & purification , Adolescent , Adult , Antigens, Viral/blood , Child , Child, Preschool , Genotype , Hepatitis A/immunology , Hepatitis A/virology , Hepatovirus/classification , Hepatovirus/genetics , Humans , Immunoenzyme Techniques , Infant , Infant, Newborn , Reverse Transcriptase Polymerase Chain Reaction , Russia/epidemiologyABSTRACT
The European Regional Office of WHO has targeted measles for elimination from the region in 2007. Large national, age and sex stratified serological surveys of measles antibody were conducted in seven Western European countries from 1994-8 as part of the European Seroepidemiology Network. Three patterns were observed in the country-specific measles seroprofiles, ranging from (very) low susceptibility (four countries) to high susceptibility (one country). Susceptibility levels amongst 2-4-year-olds ranged from 2.9 to 29.8%, in 5-9-year-olds from 2.5 to 25% and 10-19-year-olds from 2.1% to 13.9%. A country's susceptibility profile was highly associated with vaccine coverage for the first dose. First dose coverage ranged from 91 to 97.5% for low susceptibility countries, 75 to 85% for intermediate susceptibility countries and 55% for the high susceptibility country. Only the high susceptibility country still reports epidemic measles. In low susceptibility countries, which have achieved or are very close to measles elimination, the priority will be to maintain high MMR vaccine coverage in all geopolitical units for both vaccine doses. In moderate susceptibility countries there is still some endemic transmission, but also risk of outbreaks as pools of susceptibles accumulate. In the high susceptibility country the priority will be to increase infant vaccine coverage and reduce regional variation in coverage levels.
Subject(s)
Measles/epidemiology , Population Surveillance , Adolescent , Adult , Age Distribution , Child , Child, Preschool , Disease Susceptibility , Europe/epidemiology , Humans , Incidence , Infant , Measles/immunology , Measles/prevention & control , Measles-Mumps-Rubella Vaccine , Seroepidemiologic Studies , Surveys and QuestionnairesABSTRACT
OBJECTIVE: Immunization of egg-allergic children against measles, mumps, and rubella (MMR) is often deferred or even denied, although the safety of this vaccination has been clearly shown. Moreover, the majority of severe allergic reactions have occurred in egg-tolerant vaccinees. Other allergenic vaccine components have been sought, and gelatin has been suggested as one cause of allergic adverse events. The aim of this study was to further characterize the actual allergenic vaccine components. METHODS: Serum samples from 36 recipients of MMR vaccine with anaphylaxis, urticaria with or without angioedema, asthmatic symptoms, or Henoch-Schönlein purpura were analyzed by CAP System radioallergosorbent test (RAST) and immunospot methods to detect the allergenic vaccine component. To evaluate the correspondence between the findings in the CAP System RAST or the immunospot and clinical symptoms, histories of allergies and present hypersensitivity symptoms were assessed. RESULTS: Of the 36 participants, 10 were demonstrated to be allergic to gelatin. Seven of them had persistent allergic symptoms, possibly attributable to foods containing gelatin or cross-reactive allergens. The results of the immunospot suggested concomitant allergy to gelatin and egg, chicken, and feathers, as well as cow's milk, or they reflected allergen cross-reactivity. CONCLUSIONS: Although severe allergic adverse events attributable to MMR vaccination are extremely rare, all serious allergic reactions should be further assessed to detect the likely causative vaccine component, including gelatin. The current recommendation for immunization of egg-allergic persons according to standard MMR vaccination schedules is reinforced. measles, mumps, and rubella vaccine, immunization, adverse effects, allergic reactions, gelatin allergy, CAP System, radioallergosorbent test, immunospot, immunoglobulin E.
Subject(s)
Anaphylaxis/etiology , Asthma/etiology , Measles-Mumps-Rubella Vaccine/adverse effects , Urticaria/etiology , Adolescent , Adult , Animals , Chickens/immunology , Child , Child, Preschool , Female , Follow-Up Studies , Gelatin/immunology , Humans , Immunoblotting , Male , Radioallergosorbent TestABSTRACT
Most of the countries in western Europe have now implemented mass infant rubella immunization programmes, instead of or in addition to selective vaccination in order to achieve the elimination of congenital rubella syndrome. The European countries Denmark, England and Wales, Finland, France, Germany, Italy and the Netherlands undertook large, national serological surveys collecting several thousand serum specimens during 1994-8. Antibodies against rubella virus were detected by a variety of enzyme immuno-assays. Comparability of the assay results was achieved by a standardized methodology. The age- and sex-stratified serological results were related to the schedules, coverage of rubella vaccination and the incidence in these countries. The results show widely differing levels of immunity to rubella both in the general population and in the specific age groups of males and females. A low rate (< 5%) of susceptibles in childhood and adolescents of both sexes was obtained only in Finland and the Netherlands. Countries such as Italy with only moderate coverage for the infant immunization programme currently have both high susceptibility levels in the general population and in the at-risk population. The likelihood is of continued epidemics of rubella with cases of congenital rubella syndrome. The continued implementation of selective vaccination will help to offset the impact of this ongoing transmission and to protect women on reaching childbearing age.