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1.
Handb Exp Pharmacol ; (168): 445-77, 2005.
Article in English | MEDLINE | ID: mdl-16596784

ABSTRACT

Marijuana and its psychoactive constituents induce a multitude of effects on brain function. These include deficits in memory formation, but care needs to be exercised since many human studies are flawed by multiple drug abuse, small sample sizes, sample selection and sensitivity of psychological tests for subtle differences. The most robust finding with respect to memory is a deficit in working and short-term memory. This requires intact hippocampus and prefrontal cortex, two brain regions richly expressing CB1 receptors. Animal studies, which enable a more controlled drug regime and more constant behavioural testing, have confirmed human results and suggest, with respect to hippocampus, that exogenous cannabinoid treatment selectively affects encoding processes. This may be different in other brain areas, for instance the amygdala, where a predominant involvement in memory consolidation and forgetting has been firmly established. While cannabinoid receptor agonists impair memory formation, antagonists reverse these deficits or act as memory enhancers. These results are in good agreement with data obtained from electrophysiological recordings, which reveal reduction in neural plasticity following cannabinoid treatment, and increased plasticity following antagonist exposure. The mixed receptor properties of the pharmacological tool, however, make it difficult to define the exact role of any CB1 receptor population in memory processes with any certainty. This makes it all the more important that behavioural studies use selective administration of drugs to specific brain areas, rather than global administration to whole animals. The emerging role of the endogenous cannabinoid system in the hippocampus may be to facilitate the induction of long-term potentiation/the encoding of information. Administration of exogenous selective CB1 agonists may therefore disrupt hippocampus-dependent learning and memory by 'increasing the noise', rather than 'decreasing the signal' at potentiated inputs.


Subject(s)
Cannabinoids/pharmacology , Learning/drug effects , Memory/drug effects , Neuronal Plasticity/drug effects , Animals , Avoidance Learning , Fear/psychology , Hippocampus/drug effects , Hippocampus/physiology , Humans , Long-Term Potentiation/drug effects , Marijuana Abuse/psychology , Maze Learning/drug effects , Models, Animal , Neuronal Plasticity/physiology , Receptor, Cannabinoid, CB1/drug effects , Receptor, Cannabinoid, CB1/physiology , Synapses/drug effects , Synapses/physiology , Synaptic Transmission/drug effects
2.
Neuropharmacology ; 42(8): 993-1007, 2002 Jun.
Article in English | MEDLINE | ID: mdl-12128000

ABSTRACT

Marijuana smoking is recognised to impair human cognition and learning, but the mechanisms by which this occurs are not well characterised. This article focuses exclusively on the hippocampus to review the effects of cannabinoids on hippocampal function and evaluate the evidence that hippocampal cannabinoid receptors play a role in learning and formation of memory. Activation of cannabinoid receptors inhibits release of a variety of neurotransmitters, and modulates a number of intrinsic membrane conductances. Suppression of inhibitory GABAergic synaptic transmission has been repeatedly described, but whether there is also control of excitatory glutamatergic transmission is more controversial. The recognition that the commonly used WIN55,212-2 also acts via non-cannabinoid receptors may help resolve this issue. The involvement of endocannabinoids in depolarisation induced suppression of inhibition (DSI) and the demonstration that activation of metabotropic glutamate receptors can stimulate endocannabinoid release have provided the first insights into the physiological roles of the cannabinoids. Cannabinoids have consistently been reported to inhibit high frequency stimulation induced synaptic long-term potentiation but the experimental design of most behavioural experiments have meant it is not possible to categorically demonstrate a role for hippocampal cannabinoid receptors in learning and memory.


Subject(s)
Cannabinoids/metabolism , Hippocampus/physiology , Receptors, Drug/physiology , Animals , Cannabinoid Receptor Modulators , Cannabinoids/pharmacology , Cognition/drug effects , Cognition/physiology , Humans , Receptors, Cannabinoid , Signal Transduction/physiology , Synaptic Transmission/drug effects , Synaptic Transmission/physiology
3.
Neuropharmacology ; 41(8): 1000-5, 2001 Dec.
Article in English | MEDLINE | ID: mdl-11747904

ABSTRACT

We have previously reported that the synthetic cannabinoid receptor agonist WIN55,212-2 causes a selective reduction in paired-pulse depression of population spikes in the CA1 region of the rat hippocampal slice. This effect is consistent with the observation that activation of cannabinoid receptors inhibits GABA release in the hippocampus. We have now investigated the actions of the putative endogenous cannabinoids 2-arachidonoyl-glycerol (2-AG) and anandamide in this system. 2-AG mimicked the effect of WIN55,212-2 by selectively reducing paired-pulse depression at concentrations of 1-30 microM. In contrast, anandamide caused a selective increase in paired-pulse depression at concentrations of 1-30 microM. This effect was mimicked by the vanilloid receptor agonists capsaicin and resiniferatoxin, and blocked by the vanilloid receptor antagonist capsazepine, but not by the cannabinoid receptor antagonist AM281. These results are the first to demonstrate a clear functional vanilloid receptor-mediated effect in the hippocampus, and further, that anandamide but not 2-AG acts at these receptors to increase paired-pulse depression of population spikes.


Subject(s)
Arachidonic Acids/physiology , Cannabinoids , Hippocampus/metabolism , Receptors, Drug/metabolism , Action Potentials/drug effects , Action Potentials/physiology , Animals , Arachidonic Acids/metabolism , Cannabinoids/pharmacology , Capsaicin/metabolism , Capsaicin/pharmacology , Diterpenes/metabolism , Diterpenes/pharmacology , Endocannabinoids , Glycerides/physiology , Hippocampus/drug effects , In Vitro Techniques , Polyunsaturated Alkamides , Rats , Rats, Sprague-Dawley , Receptors, Drug/agonists
4.
Br J Pharmacol ; 131(3): 453-64, 2000 Oct.
Article in English | MEDLINE | ID: mdl-11015295

ABSTRACT

The aims of this study were, to use agonists selective for the 3 mGlu receptor groups to identify developmental changes in their effects, and to assess the usefulness of proposed selective antagonists as pharmacological tools. Hippocampal slices (400 microm) were prepared from neonate (9 - 14 days) and young adult (5 - 7 weeks) Sprague-Dawley rats. Field excitatory postsynaptic potentials (fEPSP) were recorded from CA1. DHPG (100 microM), a group I agonist, produced a slowly developing enhancement of fEPSP slope in slices from adults. In slices from neonates, DHPG (75 microM) depressed fEPSP slope. DCG-IV (500 nM), a group II agonist, did not affect the fEPSP recorded from slices from adults whereas perfusion in neonate slices produced a sustained depression. The group III agonist L-AP4 (50 microM) was ineffective in adult slices but depressed fEPSP slope in slices prepared from neonates. DHPG-induced depression of fEPSP slope was inhibited by 4-CPG (400 microM), a group I antagonist, but was unaffected by MCCG (500 microM) and MAP4 (500 microM), group II and III receptor antagonists respectively. MCCG but not MAP4 antagonized the effects of DCG-IV with 4-CPG producing variable effects. The effect of L-AP4 was unaffected by MCCG, blocked by MAP4, and enhanced by 4-CPG. The results show that the effects of the agonists for all groups of mGlu receptors are developmentally regulated. Furthermore, MCCG and MAP4 behave as effective and selective antagonists for group II and group III mGlu receptors respectively, whereas the usefulness of 4-CPG as a group I antagonist may be limited.


Subject(s)
Hippocampus/metabolism , Receptors, Glutamate/metabolism , Synaptic Transmission/physiology , Animals , Cyclopropanes/pharmacology , Excitatory Amino Acid Agonists/pharmacology , Excitatory Amino Acid Antagonists/pharmacology , Female , Glycine/analogs & derivatives , Glycine/pharmacology , Hippocampus/drug effects , Propionates/pharmacology , Rats , Rats, Sprague-Dawley , Resorcinols/pharmacology , Synaptic Transmission/drug effects
5.
Neuroscience ; 100(4): 789-96, 2000.
Article in English | MEDLINE | ID: mdl-11036212

ABSTRACT

An important contributor to the generation of epileptiform activity is the synchronization of burst firing in a group of neurons. The aim of this study was to investigate whether gap junctions are involved in this synchrony using an in vitro model of epileptiform activity. Hippocampal slices (400 microm) were prepared from female Sprague-Dawley rats (120-170 g). The perfusion of slices with a medium containing no added magnesium and 4-aminopyridine (50 microM) resulted in the generation of spontaneous bursts of population spikes of a fast frequency along with less frequent negative-going bursts. The frequency of the bursts produced was consistent over a 3h period. Carbenoxolone (100 microM), a gap junction blocker and mineralocorticoid agonist, perfused for 75 min, reduced the frequency of both types of spontaneous burst activity. Perfusion of spironolactone (1 microM), a mineralocorticosteroid antagonist, for 15 min prior to and during carbenoxolone perfusion did not alter the ability of carbenoxolone to depress the frequency of spontaneous activity. The incubation of hippocampal slices in carbenoxolone prior to recording increased the time taken for the spontaneous activity to start on change to the zero magnesium/4-aminopyridine medium and decreased the total number of spontaneous bursts over the first 60 min period. The ability of carbenoxolone to delay induction of epileptiform activity and reduce established epileptiform activity suggests that gap junctions contribute to the synchronization of neuronal firing in this model.


Subject(s)
Carbenoxolone/pharmacology , Epilepsy/physiopathology , Hippocampus/drug effects , Receptors, Mineralocorticoid/agonists , Animals , Depression, Chemical , Female , Gap Junctions/drug effects , Hippocampus/physiopathology , Hippocampus/ultrastructure , Hydrogen-Ion Concentration , In Vitro Techniques , Intracellular Fluid/metabolism , Mineralocorticoid Receptor Antagonists , Octanols/pharmacology , Patch-Clamp Techniques , Rats , Rats, Sprague-Dawley , Spironolactone/pharmacology , Synaptic Transmission
6.
Br J Pharmacol ; 131(4): 663-5, 2000 Oct.
Article in English | MEDLINE | ID: mdl-11030713

ABSTRACT

The cannabinoid (CB) receptor agonist WIN55,212-2 (500 nM) had no effect on the first of a pair of population spikes evoked in the CA1 region of hippocampal slices prepared from young adult (4 - 6 weeks old) rats, despite powerfully reducing paired-pulse depression. In contrast WIN55,212-2 caused a substantial depression of the single population spike (reduced to 43% control) and the field EPSP (reduced to 72% of control) recorded in slices prepared from neonatal (10 - 13 days old) rats. This effect was stereoselective and blocked by the CB(1) receptor antagonist AM281 (500 nM). The results indicate that activation of CB(1) receptors inhibits excitatory synaptic transmission in neonatal, but not adult rat hippocampus. This developmental regulation of CB(1) receptor mediated control of excitatory transmission may help explain some, but not all, of the previous discrepancies in the literature.


Subject(s)
Hippocampus/physiology , Receptors, Drug/physiology , Synaptic Transmission , Action Potentials/drug effects , Age Factors , Animals , Benzoxazines , Hippocampus/drug effects , Morpholines/pharmacology , Naphthalenes/pharmacology , Pyrazoles/pharmacology , Rats , Rats, Sprague-Dawley , Receptors, Cannabinoid
7.
Neuropharmacology ; 37(9): 1123-30, 1998 Sep.
Article in English | MEDLINE | ID: mdl-9833642

ABSTRACT

Cannabinoids cause an increase in synaptic transmission via gamma-aminobutyric acid (GABA) receptors and this may be the mechanism by which activation of CB1 receptors blocks the induction of long-term potentiation (LTP). To test this hypothesis, we used paired pulse depression (PPD) of CA1 population spike responses recorded in the rat hippocampal slice as an index of GABA-ergic feedback inhibition, to establish whether the effects of a stereoselective CB1 receptor agonist on GABA-ergic transmission and LTP were correlated. The active isomer, WIN55212-2, blocked the induction of LTP and suppressed PPD over the concentration range 250 nM-5 microM, whereas the inactive isomer, WIN55212-3, was inactive at 5 microM. The effects of 5 microM WIN55212-2 on both LTP and PPD were completely blocked by the CB1 receptor antagonist SR141716A (5 microM). The results show that the effects are correlated in that both suppression of PPD and blockade of induction of LTP are probably mediated by CBI receptors. However, the suppression in PPD suggests that WIN55212-2 caused a decrease in GABA-ergic feedback transmission which would be expected to facilitate, rather than block, the induction of LTP. We therefore conclude that the blockade of LTP by cannabinoids is not via upregulation of GABA-ergic synaptic transmission.


Subject(s)
Cannabinoids/pharmacology , Evoked Potentials/drug effects , Hippocampus/drug effects , Long-Term Potentiation/drug effects , Animals , Benzoxazines , Calcium Channel Blockers/pharmacology , Cannabinoids/antagonists & inhibitors , Electric Stimulation , Female , Hippocampus/physiology , In Vitro Techniques , Morpholines/pharmacology , Naphthalenes/pharmacology , Piperidines/pharmacology , Pyrazoles/pharmacology , Rats , Rats, Sprague-Dawley , Receptors, Drug/antagonists & inhibitors , Rimonabant
8.
Eur J Pharmacol ; 342(2-3): 213-6, 1998 Jan 26.
Article in English | MEDLINE | ID: mdl-9548388

ABSTRACT

We have previously shown that coapplication of arachidonic acid (10 microM) and (1S,3R)-1-aminocyclopentane-1,3-dicarboxylic acid (ACPD, 50 microM) evokes an enhancement of synaptic transmission in the CA1 region of the rat hippocampal slice. Here we have investigated whether the metabolites of arachidonic acid are implicated in this potentiation. Inclusion of the cyclo-oxygenase inhibitor indomethacin (10 microM) did not block the potentiation induced by coapplication of arachidonic acid and ACPD. However, the presence of either the cyclo-, lipo- and epoxygenase inhibitor 5,8,11,14-eicosatetraynoic acid (ETYA, 20 microM), or the lipoxygenase inhibitor nordihydroguaiaretic acid (10 microM), prevented the long-lasting enhancement. The results suggest that the lipoxygenase and epoxygenase metabolites of arachidonic acid may be involved in the induction of this form of synaptic potentiation.


Subject(s)
Arachidonic Acid/metabolism , Receptors, Metabotropic Glutamate/agonists , Synapses/drug effects , 5,8,11,14-Eicosatetraynoic Acid/pharmacology , Animals , Cyclooxygenase Inhibitors/pharmacology , In Vitro Techniques , Indomethacin/pharmacology , Lipoxygenase Inhibitors/pharmacology , Masoprocol/pharmacology , Rats , Receptors, Metabotropic Glutamate/metabolism
9.
Brain Res ; 767(1): 162-5, 1997 Aug 29.
Article in English | MEDLINE | ID: mdl-9365031

ABSTRACT

Perfusion of 100 microM melatonin had no effect on low frequency synaptic transmission, but prevented the induction of tetanically induced long-term potentiation (LTP) when recorded in the dendritic region of the CA1 in rat hippocampal slices. Perfusion of 100 microM melatonin in this preparation had no effect on the multiple population spikes recorded in Mg2+-free medium, and, in grease-gap recordings from the CA1-subiculum slice, 100 microM melatonin had no effect on depolarisations evoked by N-methyl-D-aspartate (NMDA) or alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA). This suggests that melatonin has the ability to prevent the formation of LTP, and that this effect is not mediated by blockade of NMDA receptors.


Subject(s)
Excitatory Amino Acid Antagonists/pharmacology , Long-Term Potentiation/drug effects , Melatonin/pharmacology , Receptors, N-Methyl-D-Aspartate/antagonists & inhibitors , Synaptic Transmission/drug effects , Animals , Culture Media , Excitatory Postsynaptic Potentials , In Vitro Techniques , Magnesium/physiology , N-Methylaspartate/pharmacology , Perfusion , Rats , alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid/pharmacology
10.
Neuropharmacology ; 36(10): 1387-92, 1997 Oct.
Article in English | MEDLINE | ID: mdl-9423926

ABSTRACT

We have investigated the effects of delta-9-tetrahydrocannabinol (delta-9-THC) on gamma-aminobutyric acid (GABA) receptor-mediated responses in a grease-gap recording preparation of the rat hippocampus. GABA, and the selective GABA(A) receptor agonist muscimol, evoked depolarizing responses with EC50 values of 8.5 mM and 17.0 microM, respectively. Responses to both of these agonists were selectively reduced by the non-competitive GABA(A) antagonist picrotoxin (5 microM), but were unaffected by the GABA(B) antagonist 2-hydroxysaclofen (500 microM). Responses evoked by the selective GABA(B) receptor agonist baclofen were not sufficiently large to analyse. The GABA uptake inhibitor, nipecotic acid (500 microM), potentiated responses to GABA, but not to muscimol. Similarly, 10-1000 nM delta-9-THC had no significant effect on the response to muscimol, whereas 1000 nM delta-9-THC significantly increased the response to GABA. Since GABA is the substrate of an avid uptake system, but muscimol is not, the results are consistent with the suggestion that delta-9-THC inhibits the uptake of GABA in the hippocampus.


Subject(s)
Antiemetics/pharmacology , Dronabinol/pharmacology , Hippocampus/drug effects , Receptors, GABA-A/drug effects , Animals , Baclofen/pharmacology , Dose-Response Relationship, Drug , Female , GABA Agonists/pharmacology , Muscimol/pharmacology , Rats , Rats, Sprague-Dawley , Receptors, GABA-A/physiology
11.
Neurosci Lett ; 213(3): 177-80, 1996 Aug 09.
Article in English | MEDLINE | ID: mdl-8873143

ABSTRACT

Repeated electroconvulsive stimulation (ECS) spaced at 48 h intervals significantly increased the synaptic response in the dentate gyrus in vivo, as measured by input/output curves, and reduced the degree of long-term potentiation (LTP) obtained following high frequency stimulation. An identical course of ECS had no effect on synaptic responses recorded in the stratum radiatum of CA1 in vitro and did not impair high frequency-induced LTP. These results suggest that either ECS has a selective effect on the sub-fields of the hippocampus or that in vitro recording techniques are unsuitable for detecting the increase in synaptic efficacy produced by the treatments.


Subject(s)
Dentate Gyrus/physiology , Neuronal Plasticity/physiology , Seizures/physiopathology , Animals , Electric Stimulation , Evoked Potentials/physiology , Long-Term Potentiation/physiology , Male , Memory/physiology , Rats , Rats, Inbred Strains
12.
Eur J Pharmacol ; 294(1): 147-54, 1995 Dec 27.
Article in English | MEDLINE | ID: mdl-8788426

ABSTRACT

Perfusion of neither the metabotropic glutamate receptor agonist (1S,3R)-1-aminocyclopentane-1,3-dicarboxylic acid (ACPD), nor arachidonic acid caused any long-term enhancement of synaptic transmission in the CA1 region of the rat hippocampal slice. However, co-perfusion of ACPD (50 microM) and arachidonic acid (10 microM) for 5 min induced a rapidly evoked and long-lasting enhancement of synaptic transmission. This enhancement persisted in the presence of D(-)-2-amino-5-phosphonopentanoic acid (40 microM) and is therefore independent of NMDA receptor activation. The potentiation was mimicked by perfusion of the phospholipase A2 activator melittin (10 micrograms/ml) for 5 or 10 min, or exogenous phospholipase A2 (1 microgram/ml) for 5 min, immediately before ACPD application. We propose a role for arachidonic acid in the induction of synaptic potentiation, possibly as a retrograde transmitter substance.


Subject(s)
Arachidonic Acid/physiology , Hippocampus/physiology , Long-Term Potentiation/physiology , Receptors, Metabotropic Glutamate/metabolism , Synaptic Transmission/physiology , Animals , Arachidonic Acid/metabolism , Cycloleucine/analogs & derivatives , Cycloleucine/pharmacology , Electric Stimulation , GABA Antagonists/pharmacology , Hippocampus/drug effects , In Vitro Techniques , Long-Term Potentiation/drug effects , Melitten/pharmacology , Phospholipases A/antagonists & inhibitors , Phospholipases A/metabolism , Phospholipases A2 , Picrotoxin/pharmacology , Rats , Receptors, Metabotropic Glutamate/agonists , Synaptic Transmission/drug effects
13.
Br J Pharmacol ; 115(6): 869-70, 1995 Jul.
Article in English | MEDLINE | ID: mdl-7582512

ABSTRACT

Incubation of rat hippocampal slices in the presence of the synthetic cannabinoid (-)-11-OH-delta 8-dimethylheptyl tetrahydrocannabinol (HU-210) (100 nM) prevented the induction of long-term potentiation (LTP). Slices co-incubated with both HU-210 (100 nM) and the cannabinoid antagonist, SR141716A (100 nM), exhibited tetanically induced LTP, comparable to control slices. Intriguingly, coincubation with HU-210 and SR141716A prevented the induction of the early, short-term phase of LTP.


Subject(s)
Cannabinoids/antagonists & inhibitors , Hippocampus/drug effects , Long-Term Potentiation/drug effects , Piperidines/pharmacology , Pyrazoles/pharmacology , Receptors, Drug/antagonists & inhibitors , Receptors, Drug/drug effects , Animals , Memory , Rats , Rats, Inbred Strains , Receptors, Cannabinoid , Rimonabant , Time Factors
14.
Brain Res ; 684(2): 165-71, 1995 Jul 03.
Article in English | MEDLINE | ID: mdl-7583218

ABSTRACT

The role that the metabotropic glutamate receptor plays in synaptic transmission is complex due to the multiple subtypes involved, which initiate a number of intracellular mechanisms. Here we have investigated the role of the metabotropic glutamate receptor in the induction of long-term potentiation (LTP). We have shown that, providing the CA3 region remains attached to the slice, it is possible to induce potentiation by bath perfusion of the metabotropic receptor agonist (1S,3R) 1-aminocyclopentane-1,3-dicarboxylic acid (ACPD) alone. The extent of the potentiation observed showed a strong negative correlation with the age of the animal from which the slices were prepared. Perfusion of ACPD was associated with an increase in the excitability of antidromically activated CA3 neurones, the appearance of spontaneous burst firing within the CA3 region, and an increased fibre volley recorded in the CA1 region. Blockade of N-methyl-D-aspartate (NMDA) receptors prevented all these effects. We suggest that the ACPD-induced potentiation of CA1 fEPSPs is an indirect effect caused by spontaneous burst firing and/or increased excitatory drive from CA3 neurones.


Subject(s)
Hippocampus/drug effects , Receptors, Metabotropic Glutamate/drug effects , Synaptic Transmission/drug effects , 2-Amino-5-phosphonovalerate/pharmacology , Animals , Dicarboxylic Acids/pharmacology , Female , Kinetics , Long-Term Potentiation , Rats , Rats, Sprague-Dawley , Time Factors
15.
Neuropharmacology ; 33(9): 1055-63, 1994 Sep.
Article in English | MEDLINE | ID: mdl-7530814

ABSTRACT

We have investigated the effects of administration of exogenous glutamate receptor agonists on the amplitude of field excitatory post-synaptic potentials (fEPSPs) evoked in the CA1 region of the rat hippocampal slice by stimulation of the Schaffer collateral-commissural fibres. L-Glutamate applied by iontophoresis or by bath perfusion (50 microM for 5 min) evoked a slowly rising increase in the amplitude of the fESPS which persisted for over 90 min. L-Glutamate induced potentiation was blocked by either D(-)-2-amino-5-phosphonopentanoic acid (40 microM) or by (RS)-alpha-methyl-4-carboxyphenylglycine (500 microM). In slices in which synaptic long-term potentiation had been saturated, iontophoretically applied L-glutamate did not induce further potentiation, but reset the fEPSP amplitude back to control levels. Iontophoretic administration of N-methyl-D-aspartate (NMDA) evoked a transient potentiation which decayed back to control levels within 90 min whereas bath perfusion of NMDA (50 microM) evoked a persistent depression. Bath perfusion of alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA, 50 microM) evoked no persistent effects. Bath administration of (1S,3R)-1-aminocyclopentane-1,3-dicarboxylic acid (ACPD, 50 or 100 microM) caused a short term depression of the fEPSP and no significant persistent effects. Perfusion of 100 microM ACPD in medium containing 1 microM picrotoxin caused a much smaller short term depression of the fEPSP and this was followed by a gradually developing and persistent potentiation.(ABSTRACT TRUNCATED AT 250 WORDS)


Subject(s)
Excitatory Amino Acid Agonists/pharmacology , Glutamic Acid/pharmacology , Hippocampus/drug effects , Synapses/drug effects , Synaptic Transmission/drug effects , Animals , Cycloleucine/analogs & derivatives , Cycloleucine/pharmacology , Evoked Potentials/drug effects , Female , In Vitro Techniques , Iontophoresis , N-Methylaspartate/pharmacology , Nerve Fibers/drug effects , Neurotoxins/pharmacology , Rats , Rats, Sprague-Dawley , Stimulation, Chemical , alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid/pharmacology
16.
Eur J Pharmacol ; 259(3): R7-8, 1994 Jul 11.
Article in English | MEDLINE | ID: mdl-7982446

ABSTRACT

Incubation of rat hippocampal slices with the synthetic cannabinoid (-)-11-OH-delta 8-dimethylheptyl tetrahydrocannabinol (HU-210) (100 nM) prevented the induction of long-term potentiation of field excitatory postsynaptic potentials recorded in the CA1 region. However, in slices incubated with its non-psychoactive (+)-isomer HU-211 (100 nM), which is reported to be an NMDA receptor antagonist, high frequency stimulation evoked a long-lasting potentiation, comparable to control slices.


Subject(s)
Cannabinoids/pharmacology , Hippocampus/drug effects , Long-Term Potentiation/drug effects , Animals , Dronabinol/analogs & derivatives , Dronabinol/pharmacology , Electric Stimulation , Hippocampus/cytology , In Vitro Techniques , Nerve Fibers/drug effects , Rats , Receptors, N-Methyl-D-Aspartate/antagonists & inhibitors , Stereoisomerism , Synapses/physiology
17.
Arch Int Pharmacodyn Ther ; 327(3): 355-62, 1994.
Article in English | MEDLINE | ID: mdl-7531425

ABSTRACT

In grease-gap recording preparations, depolarizing responses evoked by agonists are often followed by a hyperpolarization (post-depolarization hyperpolarization). We have investigated the origin of these post-depolarization hyperpolarizations in the rat CA1-subiculum slice. They were evoked by L-glutamate, N-methyl-d-aspartate or alpha-amino-3-hydroxy-5-methyl-isoxazole-4-propionate in approximately 80% of the slices tested. The post-depolarization hyperpolarizations evoked by perfusion of N-methyl-d-aspartate through the CA1 compartment of the chamber, persisted when N-methyl-d-aspartate receptors in the subicular compartment were blocked with D-2-amino-5-phosphonopentanoate. Carbachol only blocked the post-depolarization hyperpolarizations evoked by alpha-amino-3-hydroxy-5-methyl-isoxazole-4-propionate at concentrations which also blocked the depolarization. The post-depolarization hyperpolarization was selectively blocked by perfusion with Ca(2+)-free medium and by administration of ouabain, and showed a marked sensitivity to temperature. It is concluded that the post-depolarization hyperpolarizations observed in this preparation are not a consequence of diffusion of the agonist through the slice. The evidence is, however, consistent with them being generated by activation of the electrogenic Na(+)-K+ pump, although we cannot exclude an additional contribution from Ca(2+)- or voltage-dependent K+ currents.


Subject(s)
Glutamic Acid/pharmacology , Hippocampus/drug effects , N-Methylaspartate/pharmacology , alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid/pharmacology , 2-Amino-5-phosphonovalerate/pharmacology , Animals , Dose-Response Relationship, Drug , Electrophysiology , Evoked Potentials/drug effects , Female , Hippocampus/metabolism , In Vitro Techniques , Rats , Rats, Wistar , Receptors, N-Methyl-D-Aspartate/drug effects , Sodium-Potassium-Exchanging ATPase/drug effects , Sodium-Potassium-Exchanging ATPase/metabolism
18.
Br J Pharmacol ; 111(3): 951-5, 1994 Mar.
Article in English | MEDLINE | ID: mdl-7912632

ABSTRACT

1. Previous studies have suggested that a series of drugs modelled on part of the strychnine molecule interfere with the development of high pressure neurological syndrome (HPNS) and it was presumed that this effect was via an action on inhibitory glycinergic transmission. We have now used the rat hippocampal slice preparation to examine the possibility that some of these drugs might instead have an action at the strychnine-insensitive (SI) glycine binding site associated with the NMDA receptor. 2. D-2-Amino-5-phosphonovalerate (AP5) and 7-chlorokynurenate (7CK) had no significant effect on the height of the population spike recorded from the CA1 region in 1 mM Mg2+ medium, but both blocked the multiple population spikes recorded in Mg(2+)-free medium. The effect of 7CK, but not AP5, was reversed by 200 microM D-serine which is consistent with the known antagonist action of 7CK at the SI-glycine site. 3. A derivative of benzimidazole, which shows the clearest structural similarities to known SI-glycine site antagonists and ameliorates HPNS, mirrored the effects of 7CK although it was considerably less potent. 4. Gramine, which exacerbates HPNS, significantly increased the number of population spikes evoked in Mg(2+)-free medium. 5. Mephenesin, which is the most potent known drug in ameliorating HPNS, had no significant effect on the response recorded in 1 mM Mg2+ and significantly reduced the number of population spikes recorded in Mg(2+)-free medium, but this effect was only partially reversed by the addition of D-serine. 6. The results are consistent with the benzimidazole derivative, but not gramine, being an antagonist at the SI-glycine receptor. The results with mephenesin are equivocal but leave open the possibility that some of the drugs which are effective against HPNS act via an effect on excitatory NMDA receptor mediated transmission, rather than on inhibitory glycine-mediated transmission.


Subject(s)
High Pressure Neurological Syndrome/chemically induced , High Pressure Neurological Syndrome/drug therapy , N-Methylaspartate/antagonists & inhibitors , Receptors, N-Methyl-D-Aspartate/antagonists & inhibitors , 2-Amino-5-phosphonovalerate/pharmacology , Action Potentials/drug effects , Alkaloids/pharmacology , Animals , Benzimidazoles/pharmacology , Hippocampus/drug effects , Hippocampus/physiology , Indole Alkaloids , Kynurenic Acid/analogs & derivatives , Kynurenic Acid/pharmacology , Mephenesin/pharmacology , Rats , Structure-Activity Relationship
19.
Eur J Pharmacol ; 240(2-3): 325-6, 1993 Aug 24.
Article in English | MEDLINE | ID: mdl-8243550

ABSTRACT

Perfusion of the 1S,3R isomer of trans-aminocyclopentane-1,3-dicarboxylic acid (t-ACPD, 50 microM), or arachidonic acid (10 microM), for 5 min produced only depression of the field excitatory postsynaptic potential recorded in the CA1 region of rat hippocampal slices from which the CA3 region had been removed. However, perfusion of t-ACPD and arachidonic acid in combination induced a rapid potentiation of the response which in 4/6 slices was maintained for at least 90 min.


Subject(s)
Arachidonic Acid/pharmacology , Cycloleucine/analogs & derivatives , Hippocampus/drug effects , Synaptic Transmission/drug effects , Animals , Arachidonic Acid/administration & dosage , Cycloleucine/metabolism , Cycloleucine/pharmacology , Drug Synergism , Hippocampus/physiology , In Vitro Techniques , Rats , Stereoisomerism
20.
Nature ; 349(6305): 156-8, 1991 Jan 10.
Article in English | MEDLINE | ID: mdl-1846031

ABSTRACT

Neurotransmission at most excitatory synapses in the brain operates through two types of glutamate receptor termed alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionate (AMPA) and N-methyl-D-aspartate (NMDA) receptors; these mediate the fast and slow components of excitatory postsynaptic potentials respectively. Activation of NMDA receptors can also lead to a long-lasting modification in synaptic efficiency at glutamatergic synapses; this is exemplified in the CA1 region of the hippocampus, where NMDA receptors mediate the induction of long-term potentiation (LTP). It is believed that in this region LTP is maintained by a specific increase in the AMPA receptor-mediated component of synaptic transmission. We now report, however, that a pharmacologically isolated NMDA receptor-mediated synaptic response can undergo robust, synapse-specific LTP. This finding has implications for neuropathologies such as epilepsy and neurodegeneration, in which excessive NMDA receptor activation has been implicated. It adds fundamentally to theories of synaptic plasticity because NMDA receptor activation may, in addition to causing increased synaptic efficiency, directly alter the plasticity of synapses.


Subject(s)
Hippocampus/physiology , N-Methylaspartate/physiology , Synaptic Transmission , Animals , Calcium/physiology , Electrophysiology , In Vitro Techniques , Neuronal Plasticity , Rats , Receptors, AMPA , Receptors, N-Methyl-D-Aspartate/physiology , Receptors, Neurotransmitter/physiology , Synapses/physiology , Time Factors
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