Forensic Facial Comparison: Current Status, Limitations, and Future Directions.

Global escalation of crime has necessitated the use of digital imagery to aid the identification of perpetrators. Forensic facial comparison (FFC) is increasingly employed, often relying on poor-quality images. In the absence of standardized criteria, especially in terms of video recordings, verification of the methodology is needed. This paper addresses aspects of FFC, discussing relevant terminology, investigating the validity and reliability of the FISWG morphological feature list using a new South African database, and advising on standards for CCTV equipment. Suboptimal conditions, including poor resolution, unfavorable angle of incidence, color, and lighting, affected the accuracy of FFC. Morphological analysis of photographs, standard CCTV, and eye-level CCTV showed improved performance in a strict iteration analysis, but not when using analogue CCTV images. Therefore, both strict and lenient iterations should be conducted, but FFC must be abandoned when a strict iteration performs worse than a lenient one. This threshold ought to be applied to the specific CCTV equipment to determine its utility. Chance-corrected accuracy was the most representative measure of accuracy, as opposed to the commonly used hit rate. While the use of automated systems is increasing, trained human observer-based morphological analysis, using the FISWG feature list and an Analysis, Comparison, Evaluation, and Verification (ACE-V) approach, should be the primary method of facial comparison.

Sleep in two free-roaming blue wildebeest (Connochaetes taurinus), with observations on the agreement of polysomnographic and actigraphic techniques.

Most studies examining sleep in mammals are done under controlled conditions in laboratory/zoological facilities with few studies being conducted in their natural environment. It is not always possible to record sleep polysomnographically (PSG) from animals in their natural environments, as PSG is invasive, requiring the surgical implantation of electrodes on the surface of the brain. In contrast, actigraphy (ACT) has been shown to be a minimally-invasive method to objectively measure overall sleep times in some mammals, although not revealing specific sleep states. The aim of this study is two-fold, first, to measure sleep polysomnographically in free-roaming blue wildebeest (Connochaetes taurinus) under the most natural conditions possible, and second, to establish the degree of concordance between ACT and PSG recordings undertaken simultaneously in the same individuals. Here we examined sleep in the blue wildebeest, in a naturalistic setting, using both polysomnography (PSG) and actigraphy (ACT). PSG showed that total sleep time (TST) in the blue wildebeest for a 24-h period was 4.53 h (±0.12 h), 4.26 h (±0.11 h) spent in slow wave (non-REM) sleep and 0.28 h (±0.01 h) spent in rapid eye movement (REM) sleep, with 19.47 h (±0.12 h) spent in Wake. ACT showed that the blue wildebeest spent 19.23 h (±0.18 h) Active and 4.77 h (±0.18 h) Inactive. For both animals studied, a fair agreement between the two techniques for sleep scoring was observed, with approximately 45% of corresponding epochs analyzed being scored as both sleep (using PSG) and inactive (using ACT).

A Preliminary Description of the Sleep-Related Neural Systems in the Brain of the Blue Wildebeest, Connochaetes taurinus.

The current study provides a detailed qualitative description of the organization of the cholinergic, catecholaminergic, serotonergic, orexinergic, and GABAergic sleep-related systems in the brain of the blue wildebeest (Connocheates taurinus), along with a quantitative analysis of the pontine cholinergic and noradrenergic neurons, and the hypothalamic orexinergic neurons. The aim of this study was to compare the nuclear organization of these systems to other mammalian species and specifically that reported for other Cetartiodactyla. In the brain of the blue wildebeest, from the basal forebrain to the pons, the nuclear organization of the cholinergic, catecholaminergic, serotonergic, and orexinergic systems, for the most part, showed a corresponding nuclear organization to that reported in other mammals and more specifically the Cetartiodactyla. Furthermore, the description and distribution of the GABAergic system, which was examined through immunostaining for the calcium binding proteins calbindin, calretinin, and parvalbumin, was also similar to that seen in other mammals. These findings indicate that sleep in the blue wildebeest is likely to show typically mammalian features in terms of the global brain activity of the generally recognized sleep states of mammals, but Cetartiodactyl-specific features of the orexinergic system may act to lower overall daily total sleep time in relation to similar sized non-Cetartiodactyl mammals. Anat Rec, 2019. © 2019 American Association for Anatomy Anat Rec, 303:1977-1997, 2020. © 2019 American Association for Anatomy.

Evidence of the sternalis muscle in two South African cadavers.

The sternalis muscle is an infrequent, non-pathological anatomical variant typically misrepresented in a clinical context. It presents with 3-8% prevalence, according to cadaveric studies. The muscles were identified during routine cadaver prosection at the School of Anatomical Sciences, University of the Witwatersrand. Here, we report two cases of the sternalis muscle in two South African White cadavers. Analysis of the sternalis muscles revealed unilaterally present, distinctly defined muscle masses on the right (case number 1, female) and left (case number 2, male) hemithorax, lateral to the sternum. The muscles occurred with a prevalence of 2.25% within the cadaveric population examined. The prevalence of the sternalis muscle is generally low, especially in the European population. Their presence represents the remnants of the cutaneous muscles in the ventral thorax of lower animals. Clinically, the sternalis muscle may be misinterpreted as a pathological mass or lesion, thus accurate knowledge regarding its variations and prevalence is of importance.

Seasonal variations in sleep of free-ranging Arabian oryx (Oryx leucoryx) under natural hyperarid conditions.

Study Objectives: The Arabian oryx lives under hyperarid conditions in the Arabian Desert and exhibits temporal niche switching of activity patterns at a seasonal level. The objective of the current study was to provide a polysomnographic-based study of sleep in free-roaming Arabian oryx in their natural habitat to determine whether extreme seasonal climate variations resulted in changes in sleep patterns and physiology associated with the seasonal switching of temporal niches. Methods: Electroencephalography, nuchal electromyography, actigraphy, and subcutaneous temperature were recorded in free-roaming Arabian oryx in the Mahazat as-Sayd Protected Area, Kingdom of Saudi Arabia during winter and summer. Results: Total daily sleep time in winter was 6.69 and 3.77 hr in summer. In winter, oryx exhibited nocturnal sleep typical of artiodactyls of around 60 kg body mass. In summer, oryx slept mostly during the day and subcutaneous temperature was seen to rise during sleep, but not as rapidly as the rises observed in ambient air temperature. Rapid eye movement sleep formed a very small percentage of total sleep time, especially so in the summer. Conclusions: The unusual sleep patterns and physiology during summer appear to be related to high ambient air temperatures that affect both intrinsic and extrinsic factors necessary for survival. The Arabian oryx appears to use sleep physiology as an adaptive thermoregulatory mechanism in the hot summer months.

Temporal niche switching in Arabian oryx (Oryx leucoryx): Seasonal plasticity of 24h activity patterns in a large desert mammal.

The Arabian oryx, a moderately large mammal that inhabits a harsh desert environment, has been shown to exhibit seasonal variations in activity and inactivity patterns. Here we analyzed the continuous year-round activity patterns of twelve free-roaming Arabian oryx under natural conditions from two varying desert environments in Saudi Arabia using abdominally implanted activity meters. We simultaneously recorded weather parameters at both sites to determine whether environmental factors are responsible for temporal niche switching as well as the seasonal structuring and timing of this behavioural plasticity. Our results demonstrate that Arabian oryx undergo temporal niche switching of 24h activity patterns at a seasonal level and exhibit distinct nocturnal/crepuscular activity during summer, diurnal activity during winter and intermittent patterns of behaviour during the transitional seasons of autumn and spring. In addition, the oryx exhibited inter- and intra-seasonal variations in the temporal budgeting of 24h activity patterns. Strong relationships with both photoperiod and ambient temperatures were found and in some instances suggested that increasing ambient temperatures are a primary driving force behind seasonal shifts in activity patterns. These adaptive patterns may be dictated by the availability of food and water, which in turn are strongly influenced by seasonal climate variations. Overall, the adaptive responses of free-roaming Arabian oryx in such harsh and non-laboratorial conditions provide a framework for comparing wild populations as well as aiding conservation efforts.

Neurochemical organization and morphology of the sleep related nuclei in the brain of the Arabian oryx, Oryx leucoryx.

The Arabian oryx, Oryx leucoryx, is a member of the superorder Cetartiodactyla and is native to the Arabian Desert. The desert environment can be considered extreme in which to sleep, as the ranges of temperatures experienced are beyond what most mammals encounter. The current study describes the nuclear organization and neuronal morphology of the systems that have been implicated in sleep control in other mammals for the Arabian oryx. The nuclei delineated include those revealed immunohistochemically as belonging to the cholinergic, catecholaminergic, serotonergic and orexinergic systems within the basal forebrain, hypothalamus, midbrain and pons. In addition, we examined the GABAergic neurons and their terminal networks surrounding or within these nuclei. The majority of the neuronal systems examined followed the typical mammalian organizational plan, but some differences were observed: (1) the neuronal morphology of the cholinergic laterodorsal tegmental (LDT) and pedunculopontine tegmental (PPT) nuclei, as well as the parvocellular subdivision of the orexinergic main cluster, exhibited Cetartiodactyl-specific features; (2) the dorsal division of the catecholaminergic anterior hypothalamic group (A15d), which has not been reported in any member of the Artiodactyla studied to date, was present in the brain of the Arabian oryx; and (3) the catecholaminergic tuberal cell group (A12) was notably more expansive than previously seen in any other mammal. The A12 nucleus has been associated functionally to osmoregulation in other mammals, and thus its expansion could potentially be a species specific feature of the Arabian oryx given their native desert environment and the need for extreme water conservation.

Arabian Oryx (Oryx leucoryx) Respond to Increased Ambient Temperatures with a Seasonal Shift in the Timing of Their Daily Inactivity Patterns.

The Arabian oryx inhabits an environment where summer ambient temperatures can exceed 40 °C for extended periods of time. While the oryx uses a suite of adaptations that aid survival, the effects of this extreme environment on inactivity are unknown. To determine how the oryx manages inactivity seasonally, we measured the daily rhythm of body temperature and used fine-grain actigraphy, in 10 animals, to reveal when the animals were inactive in relation to ambient temperature and photoperiod. We demonstrate that during the cooler winter months, the oryx was inactive during the cooler parts of the 24-h day (predawn hours), showing a nighttime (nocturnal) inactivity pattern. In contrast, in the warmer summer months, the oryx displayed a bimodal inactivity pattern, with major inactivity bouts (those greater than 1 h) occurring equally during both the coolest part of the night (predawn hours) and the warmest part of the day (afternoon hours). Of note, the timing of the daily rhythm of body temperature did not vary seasonally, although the amplitude did change, leading to a seasonal alteration in the phase relationship between inactivity and the body temperature rhythm. Because during periods of inactivity the oryx were presumably asleep for much of the time, we speculate that the daytime shift in inactivity may allow the oryx to take advantage of the thermoregulatory physiology of sleep, which likely occurs when the animal is inactive for more than 1 h, to mitigate environmentally induced increases in body temperature.