1.
J Immunol Methods
; 288(1-2): 91-8, 2004 May.
Article
in English
| MEDLINE
| ID: mdl-15183088
ABSTRACT
We have developed a novel method of high-throughput Multiplexed Competitive Antibody Binning (MCAB). Using only a small amount of antibody and antigen, this method enables the sorting of a large, complex panel of monoclonal antibodies into different bins based on cross-competition for antigen binding. The MCAB assay builds on Luminex multiplexing bead-based technology to detect antibody competition. Because of its high sensitivity, the MCAB method is immediately applicable after identification of antigen-positive mAbs, providing information useful for advancing mAb candidates into further testing. The MCAB assay also can be used for sorting mAbs into binding groups after screening for functional activity.
Subject(s)
Antibodies, Monoclonal/analysis , Antibodies, Monoclonal/immunology , Binding, Competitive/immunology , Animals , Binding Sites, Antibody/immunology , Epitope Mapping , Epitopes/immunology , Humans , Mice
2.
Methods Mol Biol
; 248: 191-200, 2004.
Article
in English
| MEDLINE
| ID: mdl-14970497