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1.
BMC Med Educ ; 22(1): 588, 2022 Aug 01.
Article in English | MEDLINE | ID: mdl-35915499

ABSTRACT

OBJECTIVES: Dental graduates must graduate with high levels of clinical skills. Education in the clinical environment needs to be more than didactic supervision of practice by clinical teachers. Appropriate feedback in this context, is therefore critical to the development of student competence and confidence. This study was conducted to enhance and develop the assessment and feedback processes during clinical sessions in a Dental University Hospital in an effort to contribute to the development of students' self-assessment skills, reflective ability and clinical competence. METHODS: A new evidence-based model of feedback was introduced between clinical teachers and dental students. The implementation of this model was evaluated by students through a survey and focus groups. Descriptive and inferential statistics were applied to the quantitative data, while thematic analysis applied to the qualitative data. RESULTS: Findings from the survey indicated that students perceived the new model of feedback to be a positive addition to their learning experiences. The majority indicated a preference to continue using it. Quantitative analysis also demonstrated that students placed a high value on the feedback they received through the new model and associated it with improved individual performance. Five themes generated from the qualitative analysis echoed the perception that the model of feedback enhanced learning opportunities, especially when it was focused on individual performance and incorporated peer feedback. Students' preferences in relation to feedback processes were also gleamed from quantitative and qualitative analyses, that is, provision of positive and constructive feedback, both in dialogue and in written formats, delivered during and after each clinical session and addressing their individual competency learning goals for the future. Some challenges to be addressed were also identified (e.g., time constraints, inter-personal issues, and non-conducive environments). CONCLUSIONS: Feedback is central to learning and remains a complex and challenging area. By adopting effective and evidence-based feedback practices through the introduction of a feedback model, students can be supported in regulating their own learning in the clinical learning environment.


Subject(s)
Clinical Competence , Students , Feedback , Formative Feedback , Humans , Learning
2.
Folia Phoniatr Logop ; 74(4): 296-310, 2022.
Article in English | MEDLINE | ID: mdl-34530429

ABSTRACT

BACKGROUND: A fundamental component of supervising a student speech and language therapist (SLT) on placement is the provision of feedback. There are numerous identified challenges to ensure the delivery of high-quality feedback to optimise student learning and student success. Supervisors can help overcome these challenges and engage in evidence-based feedback processes if they are supported to develop the necessary knowledge and skills. E-learning is one possible means to provide this professional development to a large number of practising SLTs who are geographically dispersed and have conflicting schedules. AIMS: This study aimed to capture and evaluate the perspectives of SLTs who completed an e-learning course on providing feedback in the clinical learning environment, including the suitability and effectiveness of the e-learning tool used. METHODS AND PROCEDURES: An innovative e-learning course was designed to provide asynchronous video and interactive content on evidence-based theories and practices for effective feedback processes. Clinical scenarios relevant to the discipline of speech and language therapy were included. Participants were invited to complete optional, anonymous pre- and post-evaluation surveys. Data were analysed quantitively (descriptive and inferential statistics) and qualitatively (thematic analysis). OUTCOMES AND RESULTS: Participants indicated that the e-learning course supported them to enhance their feedback processes in the clinical learning environment through identified changes to their practices. The increases in confidence providing feedback they reported were statistically significant. In addition, the e-learning course was rated highly on numerous variables related to quality. Recommendations for adaptations and additions were also highlighted. CONCLUSIONS AND IMPLICATIONS: An e-learning course on effective and evidence-based feedback processes provides an opportunity to provide professional development to a large number of geographically dispersed practitioners in a cost-effective and flexible way. This could ensure more SLTs are confident and competent in their role as supervisor of students, which requires distinct knowledge and skills from that of a practitioner. Ultimately, this will help maximise educator and student success in the feedback process and consequently improve clinical performance and healthcare delivery.


Subject(s)
Computer-Assisted Instruction , Speech , Allied Health Personnel , Feedback , Humans , Language Therapy/methods , Speech Therapy/methods
3.
J Med Imaging Radiat Sci ; 52(4S): S57-S67, 2021 12.
Article in English | MEDLINE | ID: mdl-34261615

ABSTRACT

BACKGROUND: Clinical placement is an integral part of the Radiation Therapy undergraduate programme. Feedback and formative assessment during clinical placement are regarded as key to developing clinical skills and competencies. Students regularly report dissatisfaction with the feedback process while clinical educators report heavy clinical workloads and a lack of guidance on feedback mechanisms as barriers to providing meaningful student feedback. METHODS: An eLearning teaching intervention was developed to support radiation therapists in the provision of student feedback in the clinic. Thematic analysis was used to report attitudes to feedback and feedback practices collected in a pre and a post intervention evaluation. RESULTS: 30 radiation therapists completed the module and pre and post intervention evaluations. Prior to taking the module just over half of respondents stated that they offered regular and on-going feedback throughout the student's placement. Positive attitudes to feedback were reported. Following completion of the eLearning tool respondents reported a higher level of confidence in the provision of student feedback and almost 70% said the module had changed how they would approach the feedback process by using feedback models in the future. DISCUSSION: Good and timely feedback is essential and allows a student opportunity to improve prior to the end of the placement. It also teaches students how to self-assess and self-reflect - skills that they can use in continuous professional development after they graduate. Radiation therapists appreciate the structure that using a model in the feedback process offers. CONCLUSION: This eLearning teaching intervention was received favourably by radiation therapists who are key to creating a culture of feedback in the clinical environment that will facilitate students in becoming competent healthcare professionals.


Subject(s)
Computer-Assisted Instruction , Clinical Competence , Feedback , Humans , Students
4.
BMJ Mil Health ; 166(6): 411-413, 2020 Dec.
Article in English | MEDLINE | ID: mdl-33293376

ABSTRACT

Disease non-battle injury has plagued British expeditionary forces through the ages. While in recent years significant mortality has reduced, it has had a large impact on operational effectiveness, at times leading to closure of major medical treatment facilities (MTFs).Infection Prevention and Control (IPC) benefits from a subject matter expert and champion to ensure it remains at the front of people's minds and to be on hand to manage acute and dynamic situations. To mitigate the lack of an IPC Nursing Officer, we piloted a deployed military IPC Lead Link Practitioner (IPC-LL) for the first time on a large-scale overseas exercise (SAIF SAREEA 3). An experienced generalist nurse deploying as the IPC-LL (after specific training) provided pre-deployment IPC education and preparation, deployed IPC advice, undertook mandatory audits and monitored IPC compliance throughout the MTFs on the exercise. Data from 22 IPC audits conducted on the exercise showed that the presence of the IPC-LL improved IPC compliance and standards overall in the MTF where based, compared with others. In addition, a gastroenteritis outbreak occurred and was successfully managed with significant input from the IPC-LL. The IPC-LL was also able to add value by pre-empting potential IPC problems from occurring.There is a small pool of deployable Infection Prevention and Control Nursing Officers, so this new IPC-LL role could help to fill the capability gap. The IPC-LL could be the dedicated person focusing on IPC elements, reducing the IPC risk within the deployed field hospital setting where deployed experts are not available.


Subject(s)
Infection Control/methods , Teaching/statistics & numerical data , Disease Outbreaks/prevention & control , Humans , Infections/epidemiology , Infections/ethnology , Pilot Projects , United Kingdom/epidemiology , United Kingdom/ethnology
5.
Public Health Nurs ; 34(6): 531-540, 2017 11.
Article in English | MEDLINE | ID: mdl-28944498

ABSTRACT

OBJECTIVE: The Florida Maternal, Infant, and Early Childhood Home Visiting (MIECHV) program aims to minimize the occurrence of adverse maternal and child health outcomes for mothers deemed at-risk during pregnancy or shortly after childbirth. This study sought to understand the needs of and challenges faced by immigrant families in accessing health care through the perspective of Florida MIECHV home visitors, supervisors, and administrators. DESIGN AND SAMPLE: In this exploratory qualitative study, focus groups were held at each of the Florida MIECHV-funded program sites with a total of 81 MIECHV staff to discuss how the program addresses a range of participant needs. MEASURES: Data were collected through 32 semi-structured focus groups. Transcripts were analyzed using a hybrid approach entailing the development of an a priori codebook and thematic analysis. RESULTS: Staff from eight of the 11 programs described social and physical isolation and economic hardship faced by immigrant families enrolled in their programs, resulting in barriers to needed health care and social services. CONCLUSIONS: Home visitors in the Florida MIECHV program served as trusted confidants that helped families navigate social services. Future research should focus on the impact that home visiting has on immigrant health and whether this impact is maintained over time.


Subject(s)
Administrative Personnel/psychology , Emigrants and Immigrants , Health Status , House Calls , Maternal-Child Health Services/organization & administration , Administrative Personnel/statistics & numerical data , Adult , Child, Preschool , Female , Florida , Focus Groups , Health Services Accessibility/economics , Health Services Needs and Demand , Humans , Infant , Infant, Newborn , Poverty , Pregnancy , Program Evaluation , Qualitative Research , Social Isolation , Young Adult
6.
Proc Natl Acad Sci U S A ; 108(46): E1146-55, 2011 Nov 15.
Article in English | MEDLINE | ID: mdl-22006328

ABSTRACT

Autosomal dominant hypophosphatemic rickets (ADHR) is unique among the disorders involving Fibroblast growth factor 23 (FGF23) because individuals with R176Q/W and R179Q/W mutations in the FGF23 (176)RXXR(179)/S(180) proteolytic cleavage motif can cycle from unaffected status to delayed onset of disease. This onset may occur in physiological states associated with iron deficiency, including puberty and pregnancy. To test the role of iron status in development of the ADHR phenotype, WT and R176Q-Fgf23 knock-in (ADHR) mice were placed on control or low-iron diets. Both the WT and ADHR mice receiving low-iron diet had significantly elevated bone Fgf23 mRNA. WT mice on a low-iron diet maintained normal serum intact Fgf23 and phosphate metabolism, with elevated serum C-terminal Fgf23 fragments. In contrast, the ADHR mice on the low-iron diet had elevated intact and C-terminal Fgf23 with hypophosphatemic osteomalacia. We used in vitro iron chelation to isolate the effects of iron deficiency on Fgf23 expression. We found that iron chelation in vitro resulted in a significant increase in Fgf23 mRNA that was dependent upon Mapk. Thus, unlike other syndromes of elevated FGF23, our findings support the concept that late-onset ADHR is the product of gene-environment interactions whereby the combined presence of an Fgf23-stabilizing mutation and iron deficiency can lead to ADHR.


Subject(s)
Familial Hypophosphatemic Rickets/genetics , Fibroblast Growth Factors/genetics , Iron Deficiencies , Anemia, Iron-Deficiency/complications , Animals , Familial Hypophosphatemic Rickets/physiopathology , Female , Fibroblast Growth Factor-23 , Gene-Environment Interaction , Glucuronidase/metabolism , Hypophosphatemia/genetics , Klotho Proteins , MAP Kinase Signaling System , Male , Mice , Mice, Transgenic , Osteocytes/cytology , Osteomalacia/genetics , Phenotype , Protein Structure, Tertiary , Rats
7.
J Am Soc Nephrol ; 20(5): 955-60, 2009 May.
Article in English | MEDLINE | ID: mdl-19357251

ABSTRACT

Fibroblast growth factor-23 (FGF23), a hormone central to phosphate and vitamin D metabolism, reduces renal absorption of phosphate by downregulating the sodium-phosphate cotransporter Npt2a. However, the mechanisms of FGF23 action in the kidney are unclear, as Npt2a localizes to the proximal tubule (PT) and the FGF23 coreceptor alpha-Klotho (KL) localizes to the distal convoluted tubule (DCT). Immunofluorescent analyses following FGF23 injection in mice showed robust staining for phospho-ERK1/2, a marker of FGF23 bioactivity, only within the DCT in a subset of KL-positive cells. This activity colocalized with the FGF23 receptor FGFR1 and was present in DCT cells that were adjacent to Npt2a-expressing PT segments. Although KL is expressed as both secreted and membrane-bound isoforms, only the membrane-bound isoform was capable of mediating FGF23 bioactivity. These findings provide novel insight into the mechanisms of hormone-regulated phosphate metabolism by identifying an intrarenal signaling axis for FGF23.


Subject(s)
Fibroblast Growth Factors/physiology , Kidney Tubules, Distal/physiology , Sodium-Phosphate Cotransporter Proteins/physiology , Actins/genetics , Animals , Cell Line , DNA Primers , Early Growth Response Protein 1/genetics , Fibroblast Growth Factor-23 , Fibroblast Growth Factors/pharmacology , Glucuronidase/physiology , Humans , Kidney , Kidney Tubules, Distal/enzymology , Klotho Proteins , Mice , Mice, Inbred C57BL , Receptor, Fibroblast Growth Factor, Type 1/genetics , Receptor, Fibroblast Growth Factor, Type 1/metabolism , Recombinant Proteins/pharmacology , Reverse Transcriptase Polymerase Chain Reaction , Signal Transduction/physiology
8.
Bone ; 44(2): 287-94, 2009 Feb.
Article in English | MEDLINE | ID: mdl-19007919

ABSTRACT

We previously demonstrated that the mutations Met1Val (M1V) and the deletion of nucleotides 1484-1490 (1484-1490del) in Dentin matrix protein-1 (DMP1) cause the novel disorder autosomal recessive hypophosphatemic rickets (ARHR), which is associated with elevated fibroblast growth factor-23 (FGF23). To further understand the role of DMP1 in ARHR, we undertook molecular genetic and in vitro expression studies. First, we examined a kindred with a severe hypophosphatemic rickets phenotype and recessive inheritance. Analyses of this family demonstrated that the affected members had elevated serum FGF23 and carried a large, biallelic deletion that removed the majority of DMP1. At a minimum, this deletion encompassed 49 kb between DMP1 exon 3 and an intergenic region 5' to the next telomeric gene, integrin-binding sialoprotein (IBSP). We next performed immunofluorescent studies in cells to understand the effects of the known ARHR mutations on DMP1 cellular processing. These analyses showed that the M1V DMP1 mutant was not sorted to the trans-Golgi network (TGN) and secretory pathway, but filled the entire cytoplasm. In contrast, the 1484-1490del mutant localized to the TGN and was secreted, similar to wild type DMP1. The 1484-1490del mutation replaces the DMP1 18 C-terminal amino acids with 33 non-native residues. Truncation of wild type DMP1 by these native 18 residues followed by Western blot and confocal microscopic analyses demonstrated a wild type expression pattern when compared with the 1484-1490del mutant, indicating that the last 18 residues are not critical for cellular trafficking, but that the 33 additional residues arising from the 1484-1490del mutation likely compromise DMP1 processing. The relationship between DMP1 and FGF23 is unclear. To test endogenous DMP1 response to serum metabolites that also regulate FGF23, UMR-106 cells were treated with 1,25(OH)(2) vitamin D (1x10(-7) M) and showed a 12-fold increase in DMP1 mRNA and protein at 24 h. In summary, we have identified a novel DMP1 deletion as the cause of ARHR, as well as demonstrated that the ARHR mutations alter DMP1 cellular processing, and that DMP1 can be regulated by vitamin D. Taken together, this work expands our understanding of the genetic and molecular mechanisms associated with DMP1 alterations causing ARHR.


Subject(s)
Extracellular Matrix Proteins/genetics , Familial Hypophosphatemic Rickets/genetics , Genes, Recessive , Mutation/genetics , Phosphoproteins/genetics , Cell Line , Conserved Sequence , Extracellular Matrix Proteins/chemistry , Familial Hypophosphatemic Rickets/blood , Fibroblast Growth Factor-23 , Humans , Mutant Proteins/metabolism , Phosphoproteins/chemistry , Protein Transport/drug effects , Subcellular Fractions/drug effects , Subcellular Fractions/metabolism , Vitamin D/pharmacology
9.
Am J Physiol Endocrinol Metab ; 295(4): E929-37, 2008 Oct.
Article in English | MEDLINE | ID: mdl-18682534

ABSTRACT

Fibroblast growth factor 23 (FGF23) is a hormone required for normal renal phosphate reabsorption. FGF23 gain-of-function mutations result in autosomal dominant hypophosphatemic rickets (ADHR), and FGF23 loss-of-function mutations cause familial hyperphosphatemic tumoral calcinosis (TC). In this study, we identified a novel recessive FGF23 TC mutation, a lysine (K) substitution for glutamine (Q) (160 C>A) at residue 54 (Q54K). To understand the molecular consequences of all known FGF23-TC mutants (H41Q, S71G, M96T, S129F, and Q54K), these proteins were stably expressed in vitro. Western analyses revealed minimal amounts of secreted intact protein for all mutants, and ELISA analyses demonstrated high levels of secreted COOH-terminal FGF23 fragments but low amounts of intact protein, consistent with TC patients' FGF23 serum profiles. Mutant protein function was tested and showed residual, yet decreased, bioactivity compared with wild-type protein. In examining the role of the FGF23 COOH-terminal tail (residues 180-251) in protein processing and activity, truncated mutants revealed that the majority of the residues downstream from the known FGF23 SPC protease site ((176)RXXR(179)/S(180)) were not required for protein secretion. However, residues adjacent to the RXXR site (between residues 188 and 202) were required for full bioactivity. In summary, we report a novel TC mutation and demonstrate a common defect of reduced FGF23 stability for all known FGF23-TC mutants. Finally, the majority of the COOH-terminal tail of FGF23 is not required for protein secretion but is required for full bioactivity.


Subject(s)
Calcinosis/genetics , Fibroblast Growth Factors/genetics , Fibroblast Growth Factors/metabolism , Hyperphosphatemia/genetics , Mutation/physiology , Adult , Blotting, Western , Calcinosis/metabolism , Calcinosis/surgery , Calcitriol/blood , Child , Child, Preschool , DNA Mutational Analysis , Early Growth Response Protein 1/biosynthesis , Early Growth Response Protein 1/genetics , Exons/genetics , Extracellular Signal-Regulated MAP Kinases/metabolism , Fibroblast Growth Factor-23 , Humans , Hyperphosphatemia/metabolism , Male , Molecular Biology , Mutagenesis, Site-Directed , N-Acetylgalactosaminyltransferases/genetics , Parathyroid Hormone/blood , Reverse Transcriptase Polymerase Chain Reaction , Polypeptide N-acetylgalactosaminyltransferase
10.
Am J Med Genet A ; 143A(20): 2390-6, 2007 Oct 15.
Article in English | MEDLINE | ID: mdl-17853462

ABSTRACT

Familial tumoral calcinosis (TC) is characterized by elevated serum phosphate concentrations, normal or elevated 1,25(OH)2 vitamin D, as well as periarticular and vascular calcifications. Recessive mutations in the mucin-like glycosyltransferase GalNAc transferase-3 (GALNT3) and the phosphaturic hormone fibroblast growth factor-23 (FGF23) have been shown to result in TC. In the present study, mutational analyses were performed on two patients with TC to determine the molecular basis of their diseases. Analysis of the first patient revealed a novel, homozygous base insertion (1102_1103insT) in GALNT3 exon 5 that results in a frameshift and premature stop codon (E375X). The second patient had a novel homozygous transition (1460 g>a) in GALNT3 exon 7, which caused a nonsense mutation (W487X). Both mutations are predicted to markedly truncate the mature GALNT3 protein product. Although the patients carry GALNT3 mutations, these individuals presented with low-normal serum concentrations of intact biologically active FGF23 and high levels of C-terminal FGF23. In order to discern a possible relationship between GALNT3 and FGF23 in TC, a comprehensive assessment of the reported TC mutations was also performed. In summary, we have detected novel GALNT3 mutations that result in familial TC, and show that disturbed serum FGF23 concentrations are present in our TC cases as well as in previously reported cases. These studies expand our current genetic understanding of familial TC, and support a pathophysiological association between GALNT3 and FGF23.


Subject(s)
Calcinosis/genetics , Mutation , N-Acetylgalactosaminyltransferases/genetics , Amino Acid Sequence , Base Sequence , Calcinosis/pathology , DNA Mutational Analysis , Fibroblast Growth Factor-23 , Fibroblast Growth Factors/blood , Fibroblast Growth Factors/genetics , Fibroblast Growth Factors/metabolism , Humans , Molecular Sequence Data , N-Acetylgalactosaminyltransferases/blood , N-Acetylgalactosaminyltransferases/metabolism , Polypeptide N-acetylgalactosaminyltransferase
11.
Nat Genet ; 38(11): 1310-5, 2006 Nov.
Article in English | MEDLINE | ID: mdl-17033621

ABSTRACT

The osteocyte, a terminally differentiated cell comprising 90%-95% of all bone cells, may have multiple functions, including acting as a mechanosensor in bone (re)modeling. Dentin matrix protein 1 (encoded by DMP1) is highly expressed in osteocytes and, when deleted in mice, results in a hypomineralized bone phenotype. We investigated the potential for this gene not only to direct skeletal mineralization but also to regulate phosphate (P(i)) homeostasis. Both Dmp1-null mice and individuals with a newly identified disorder, autosomal recessive hypophosphatemic rickets, manifest rickets and osteomalacia with isolated renal phosphate-wasting associated with elevated fibroblast growth factor 23 (FGF23) levels and normocalciuria. Mutational analyses showed that autosomal recessive hypophosphatemic rickets family carried a mutation affecting the DMP1 start codon, and a second family carried a 7-bp deletion disrupting the highly conserved DMP1 C terminus. Mechanistic studies using Dmp1-null mice demonstrated that absence of DMP1 results in defective osteocyte maturation and increased FGF23 expression, leading to pathological changes in bone mineralization. Our findings suggest a bone-renal axis that is central to guiding proper mineral metabolism.


Subject(s)
Extracellular Matrix Proteins/genetics , Minerals/metabolism , Osteocytes/physiology , Osteomalacia/genetics , Phosphoproteins/genetics , Rickets/genetics , Adult , Animals , Bone and Bones/pathology , Calcification, Physiologic/genetics , Calcification, Physiologic/physiology , Cells, Cultured , DNA Mutational Analysis , Female , Fibroblast Growth Factor-23 , Fibroblast Growth Factors/blood , Humans , Kidney/metabolism , Male , Mice , Mice, Inbred C57BL , Mice, Knockout , Osteocytes/pathology , Osteomalacia/blood , Osteomalacia/pathology , Phosphates/metabolism , Rickets/blood , Rickets/pathology
12.
J Clin Endocrinol Metab ; 91(10): 4037-42, 2006 Oct.
Article in English | MEDLINE | ID: mdl-16868048

ABSTRACT

CONTEXT: Familial tumoral calcinosis (TC) results from disruptions in phosphate metabolism and is characterized by high serum phosphate with normal or elevated 1,25 dihydroxyvitamin vitamin D concentrations and ectopic and vascular calcifications. Recessive loss-of-function mutations in UDP-N-acetyl-alpha-D-galactosamine-polypeptide N-acetylgalactosaminyltransferase 3 (GALNT3) and fibroblast growth factor-23 (FGF23) result in TC. OBJECTIVE: The objective of the study was to determine the relationship between GALNT3 and FGF23 in familial TC. DESIGN, SETTING, AND PATIENTS: We assessed the major biochemical defects and potential genes involved in patients with TC. INTERVENTION: Combination therapy consisted of the phosphate binder Sevelamer and the carbonic anhydrase inhibitor acetazolamide. RESULTS: We report a patient homozygous for a GALNT3 exon 1 deletion, which is predicted to truncate the encoded protein. This patient had high serum FGF23 concentrations when assessed with a C-terminal FGF23 ELISA but low-normal FGF23 levels when tested with an ELISA for intact FGF23 concentrations. Matrix extracellular phosphoglycoprotein has been identified as a possible regulator of phosphate homeostasis. Serum matrix extracellular phosphoglycoprotein levels, however, were normal in the family with GALNT3-TC and a kindred with TC carrying the FGF23 S71G mutation. The tumoral masses of the patient with GALNT3-TC completely resolved after combination therapy. CONCLUSIONS: Our findings demonstrate that GALNT3 inactivation in patients with TC leads to inadequate production of biologically active FGF23 as the most likely cause of the hyperphosphatemic phenotype. Furthermore, combination therapy may be effective for reducing the tumoral burden associated with familial TC.


Subject(s)
Calcinosis/genetics , Extracellular Matrix Proteins/blood , Fibroblast Growth Factors/blood , Glycoproteins/blood , Mutation , N-Acetylgalactosaminyltransferases/genetics , Neoplasm Proteins/genetics , Phosphates/blood , Phosphoproteins/blood , Amino Acid Sequence , Base Sequence , Calcinosis/blood , Calcinosis/therapy , Calcitriol/blood , Fibroblast Growth Factor-23 , Fibroblast Growth Factors/genetics , Humans , Molecular Sequence Data , N-Acetylgalactosaminyltransferases/physiology , Neoplasm Proteins/physiology , Polypeptide N-acetylgalactosaminyltransferase
14.
Endocrinology ; 146(11): 4647-56, 2005 Nov.
Article in English | MEDLINE | ID: mdl-16081635

ABSTRACT

Fibroblast growth factor (FGF)-23 has emerged as an endocrine regulator of phosphate and of vitamin D metabolism. It is produced in bone and, unlike other FGFs, circulates in the bloodstream to ultimately regulate phosphate handling and vitamin D production in the kidney. Presently, it is unknown which of the seven principal FGF receptors (FGFRs) transmits FGF23 biological activity. Furthermore, the molecular basis for the endocrine mode of FGF23 action is unclear. Herein, we performed surface plasmon resonance and mitogenesis experiments to comprehensively characterize receptor binding specificity. Our data demonstrate that FGF23 binds and activates the c splice isoforms of FGFR1-3, as well as FGFR4, but not the b splice isoforms of FGFR1-3. Interestingly, highly sulfated and longer glycosaminoglycan (GAG) species were capable of promoting FGF23 mitogenic activity. We also show that FGF23 induces tyrosine phosphorylation and inhibits sodium-phosphate cotransporter Npt2a mRNA expression using opossum kidney cells, a model kidney proximal tubule cell line. Removal of cell surface GAGs abolishes the effects of FGF23, and exogenous highly sulfated GAG is capable of restoring FGF23 activity, suggesting that proximal tubule cells naturally express GAGs that are permissive for FGF23 action. We propose that FGF23 signals through multiple FGFRs and that the unique endocrine actions of FGF23 involve escape from FGF23-producing cells and circulation to the kidney, where highly sulfated GAGs most likely act as cofactors for FGF23 activity. Our biochemical findings provide important insights into the molecular mechanisms by which dysregulated FGF23 signaling leads to disorders of hyper- and hypophosphatemia.


Subject(s)
Endocrine System/physiology , Fibroblast Growth Factors/physiology , Animals , Cell Line , Down-Regulation , Fibroblast Growth Factor-23 , Fibroblast Growth Factors/metabolism , Fibroblast Growth Factors/pharmacology , Glycosaminoglycans/metabolism , Heparin/chemistry , Heparin/pharmacology , Humans , Kidney/cytology , Kidney/enzymology , Phosphorylation/drug effects , Polymers/pharmacology , Protein-Tyrosine Kinases/metabolism , RNA, Messenger/metabolism , Receptors, Fibroblast Growth Factor/metabolism , Signal Transduction/physiology
15.
Endocrinology ; 146(9): 3883-91, 2005 Sep.
Article in English | MEDLINE | ID: mdl-15961556

ABSTRACT

Familial tumoral calcinosis (TC, OMIM 211900) is a heritable disorder characterized by hyperphosphatemia, normal or elevated serum 1,25-dihydroxyvitamin D, and often severe ectopic calcifications. Two recessive mutations in fibroblast growth factor-23 (FGF23), serine 71/glycine (S71G) and serine 129/phenylalanine (S129F), were identified as causing TC. Herein, we undertook comprehensive biochemical analyses of an extended TC family carrying the S71G FGF23 mutation, which revealed that heterozygous (serine/glycine, S/G) individuals had elevated serum FGF23 C-terminal fragments compared with wild-type (serine/serine, S/S) family members (P < 0.025). To understand the differential processing of FGF23 in TC patients, we transiently expressed S71G as well as S129F FGF23. FGF23 ELISA in tandem with Western analyses revealed increased proteolytic cleavage of mutant FGF23 and a limited secretion of intact protein. Furthermore, S71G and S129F FGF23 carrying mutations that disrupt the furin-like protease RXXR motif in FGF23 rescued the secretion of the intact protein, and both TC mutant proteins harboring the R176Q mutation revealed no altered sensitivity to trypsin compared with the native (R176Q)FGF23. Finally, S71G, but not S129F mutant FGF23, is rescued by temperature. In summary, FGF23 mutations causing TC lead to increased intracellular proteolysis of FGF23, most likely by furin-like proteases, due to conformational changes of the mutant protein. The destabilizing nature of these mutations provides new insight into the pathophysiology of TC and exemplifies the physiological importance of FGF23 in phosphate and vitamin D metabolism.


Subject(s)
Calcinosis/genetics , Calcinosis/metabolism , Fibroblast Growth Factors/genetics , Fibroblast Growth Factors/metabolism , Cell Line , DNA, Complementary , Female , Fibroblast Growth Factor-23 , Fibroblast Growth Factors/chemistry , Genotype , Humans , Kidney/cytology , Male , Models, Molecular , Pedigree , Phenotype , Point Mutation , Protein Structure, Secondary , Temperature
16.
Bone ; 36(6): 971-7, 2005 Jun.
Article in English | MEDLINE | ID: mdl-15869926

ABSTRACT

Fibroblast growth factor-23 (FGF23) is a circulating factor that plays critical roles in phosphate and vitamin D metabolism. The goal of our studies was to dissect the pathways directing the vitamin D-phosphate-FGF23 homeostatic axis. To test the role of diet in the regulation of Fgf23, wild-type (WT) mice were fed either a standard (0.44% phosphorus) or a low-phosphate (0.02%) diet. WT mice on standard diet had a serum phosphate of 9.5 +/- 0.3 mg/dl and an Fgf23 concentration of 99.0 +/- 10.6 pg/ml; mice on the low-phosphate diet had a phosphate of 5.0 +/- 0.2 mg/dl (P < 0.01) and an Fgf23 of 10.6 +/- 3.7 pg/ml (P < 0.01). To genetically separate the effects of phosphate and vitamin D on Fgf23, we examined vitamin D receptor null (VDR(-/-)) mice, which are hypocalcemic and hypophosphatemic secondary to hyperparathyroidism. On standard diets, WT and VDR(+/-) mice had Fgf23 levels of 106.0 +/- 30.7 and 90.6 +/- 17.3 pg/ml, respectively, whereas Fgf23 was undetectable in the VDR(-/-). Animals were then placed on a diet that normalizes serum calcium and phosphorus. This 'rescue' increased Fgf23 in WT to 192.3 +/- 32.5 pg/ml and in VDR(+/-) to 388.2 +/- 89.6pg/ml, and importantly, in VDR(-/-) to 476.9 +/- 60.1 pg/ml (P < 0.01 vs. WT). In addition, renal vitamin D 1-alpha hydroxylase (1alpha-OHase) mRNA levels were corrected to WT levels in the VDR(-/-) mice. In summary, Fgf23 is suppressed in diet-induced hypophosphatemia and in hypophosphatemia associated with secondary hyperparathyroidism. Normalization of serum phosphate by diet in VDR(-/-) mice increases Fgf23. Thus, our results demonstrate that Fgf23 is independently regulated by phosphate and by vitamin D.


Subject(s)
Fibroblast Growth Factors/blood , Phosphates/physiology , Vitamin D/physiology , Animals , Calcium/blood , Calcium/pharmacology , Fibroblast Growth Factor-23 , Fibroblast Growth Factors/metabolism , Gene Expression/drug effects , Hypophosphatasia/blood , Hypophosphatasia/metabolism , Lactose/pharmacology , Mice , Mice, Inbred C57BL , Mice, Knockout , Phosphates/metabolism , Phosphorus/blood , Phosphorus/deficiency , Phosphorus, Dietary/administration & dosage , Phosphorus, Dietary/blood , Receptors, Calcitriol/genetics , Steroid Hydroxylases/genetics , Vitamin D/metabolism
17.
J Clin Endocrinol Metab ; 90(4): 2424-7, 2005 Apr.
Article in English | MEDLINE | ID: mdl-15687325

ABSTRACT

Gain-of-function mutations in fibroblast growth factor-23 (FGF23) are responsible for autosomal dominant hypophosphatemic rickets, a disorder of isolated renal phosphate wasting. Patients with the disorder display hypophosphatemia with normocalcemia as well as inappropriately normal 1,25-dihydroxyvitamin D [1,25(OH)2D3] concentrations. Reciprocally tumoral calcinosis (TC) patients are often hyperphosphatemic with inappropriately normal or elevated serum 1,25(OH)2D3 levels and have ectopic and vascular calcifications, a phenotype similar to that of Fgf23 null mice. Therefore, the goal of the present studies was to test whether FGF23 was a candidate gene for TC. Two sisters in a consanguineous TC family had hyperphosphatemia and normal 1,25(OH)2D3 levels with characteristic ectopic and vascular calcifications. Interestingly, these patients had low-normal intact serum FGF23 levels but demonstrated FGF23 concentrations approximately 40 times normal when assessed with a C-terminal FGF23 serum assay. Mutational analyses identified a homozygous S71G mutation in FGF23 in the TC patients, which was not found in control alleles. Finally, modeling demonstrated that the S71G mutation most likely destabilizes full-length FGF23. In summary, recessive FGF23 mutations can lead to TC. Additionally, our findings indicate that FGF23 may adopt an unstable conformation in some TC patients, possibly leading to nonfunctional FGF23 protein.


Subject(s)
Calcinosis/genetics , Fibroblast Growth Factors/genetics , Mutation , Phosphates/blood , Adult , Female , Fibroblast Growth Factor-23 , Fibroblast Growth Factors/chemistry , Genes, Recessive , Humans , Male , Middle Aged , N-Acetylgalactosaminyltransferases/genetics , Neoplasm Proteins/genetics , Pedigree , Phenotype , Polypeptide N-acetylgalactosaminyltransferase
18.
Am J Hum Genet ; 76(2): 361-7, 2005 Feb.
Article in English | MEDLINE | ID: mdl-15625620

ABSTRACT

Activating mutations in the genes for fibroblast growth factor receptors 1-3 (FGFR1-3) are responsible for a diverse group of skeletal disorders. In general, mutations in FGFR1 and FGFR2 cause the majority of syndromes involving craniosynostosis, whereas the dwarfing syndromes are largely associated with FGFR3 mutations. Osteoglophonic dysplasia (OD) is a "crossover" disorder that has skeletal phenotypes associated with FGFR1, FGFR2, and FGFR3 mutations. Indeed, patients with OD present with craniosynostosis, prominent supraorbital ridge, and depressed nasal bridge, as well as the rhizomelic dwarfism and nonossifying bone lesions that are characteristic of the disorder. We demonstrate here that OD is caused by missense mutations in highly conserved residues comprising the ligand-binding and transmembrane domains of FGFR1, thus defining novel roles for this receptor as a negative regulator of long-bone growth.


Subject(s)
Bone Diseases, Developmental/genetics , Face/abnormalities , Mutation, Missense , Receptor Protein-Tyrosine Kinases/genetics , Receptors, Fibroblast Growth Factor/genetics , Skull/abnormalities , Adult , Amino Acid Sequence , DNA Mutational Analysis , Humans , Karyotyping , Male , Maxillofacial Development/genetics , Middle Aged , Molecular Sequence Data , Pedigree , Receptor, Fibroblast Growth Factor, Type 1
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