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1.
J Invertebr Pathol ; 127: 32-4, 2015 May.
Article in English | MEDLINE | ID: mdl-25736726

ABSTRACT

Sugarcane borer (Diatraea saccharalis, F.) is an important corn pest in South America and United States. The aim of the present study was to analyze the susceptibility and binding interactions of three Cry1A proteins and Cry1Fa in a Brazilian D. saccharalis population. The results showed that Cry1Ab was the most active, followed by Cry1Ac, Cry1Fa and Cry1Aa. All Cry1-biotinylated proteins tested bound specifically to the D. saccharalis brush border membrane vesicles (BBMV). Heterologous competition assays showed shared binding sites for all Cry1A proteins and another one shared by Cry1Fa and Cry1Ab. Thus, pyramiding Cry1Aa/Cry1Ac and Cry1F proteins would be a recommended strategy for managing this pest.


Subject(s)
Bacterial Proteins/metabolism , Endotoxins/metabolism , Hemolysin Proteins/metabolism , Insecticide Resistance/physiology , Moths/metabolism , Pest Control, Biological/methods , Animals , Bacillus thuringiensis , Bacillus thuringiensis Toxins
2.
Biotechnol Lett ; 31(12): 1891-7, 2009 Dec.
Article in English | MEDLINE | ID: mdl-19636520

ABSTRACT

Some pest management programs employ PCR to identify cry1 genes from Bacillus thuringiensis to predict bacterial toxicity towards different insect pests. However, due to changes on the mode of action of the Cry proteins, new primers had to be designed to detect the new genes. Therefore, an 'in-silico' study of genetic sequences from five cry1 subclasses was carried out and characterized by molecular tools. The design of new primers allows for more precise selection of B. thuringiensis isolates, helping to better direct the programs employing biological control.


Subject(s)
Bacillus thuringiensis/genetics , Bacterial Proteins/genetics , DNA Primers/genetics , Endotoxins/genetics , Hemolysin Proteins/genetics , Polymerase Chain Reaction/methods , Bacillus thuringiensis Toxins , Base Sequence , DNA, Bacterial/genetics , Genotype , Molecular Sequence Data , Sensitivity and Specificity , Sequence Alignment
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