Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 13 de 13
Filter
Add more filters










Publication year range
1.
J Neuroendocrinol ; 15(12): 1156-63, 2003 Dec.
Article in English | MEDLINE | ID: mdl-14636177

ABSTRACT

It has long been known that under intracellular conditions vasopressin associates tightly to neurophysin, which is present in the same prohormone. As the association has been suggested to play a role during hormone biosynthesis, its role was studied in a cellular context by expressing mutant vasopressin precursors in Neuro2A cells. Mutant vasopressin precursors, in which the association between the vasopressin and neurophysin domains was prevented either by deleting the vasopressin domain from the precursor or by substitution of the essential Tyr2 residue in vasopressin for Gly, were neither processed nor targeted into secretory granules. Rather, both provasopressin mutants were retained in the endoplasmic reticulum. Our results demonstrate that the vasopressin domain is crucial for correct trafficking of the prohormone through the secretory pathway, and suggest that vasopressin-neurophysin association provides correct prohormone folding in the endoplasmic reticulum.


Subject(s)
Protein Transport/physiology , Vasopressins/genetics , Vasopressins/metabolism , Animals , Cell Line, Tumor , Diabetes Insipidus/metabolism , Diabetes Insipidus/physiopathology , Endoplasmic Reticulum/metabolism , Gene Expression , Mutagenesis , Neuroblastoma , Neurophysins/chemistry , Neurophysins/genetics , Protein Folding , Protein Structure, Tertiary , Rats , Secretory Vesicles/metabolism , Vasopressins/chemistry
3.
J Neuroendocrinol ; 12(6): 589-94, 2000 Jun.
Article in English | MEDLINE | ID: mdl-10844588

ABSTRACT

The trafficking of prohormones and of regulated secretory proteins in general has been studied extensively in the last decades of the last century. Prohormone trafficking starts with correct folding and subsequently efficient sorting into the secretory granule of the regulated secretory pathway. The vasopressin/oxytocin prohormone is particularly interesting for studying protein trafficking, because the physicochemical properties and three-dimensional structure have been largely elucidated. In the case of pro-vasopressin and pro-oxytocin, folding and sorting depend completely on both intramolecular and intermolecular interactions. Proper folding is guided by the hormone-neurophysin association and the sorting event relies on the aggregative properties of the neurophysin domain in the prohormone, as well as a specific sorting signal, which is revealed when the aggregative property of the neurophysin domain is deleted. A comprehensive mechanism for trafficking of the vasopressin/oxytocin prohormone from the endoplasmic reticulum to the secretory granule is proposed.


Subject(s)
Oxytocin/metabolism , Protein Precursors/metabolism , Vasopressins/metabolism , Amino Acid Sequence/genetics , Animals , Humans , Neurophysins/chemistry , Neurophysins/physiology , Oxytocin/chemistry , Oxytocin/genetics , Protein Folding , Protein Precursors/chemistry , Protein Precursors/genetics , Vasopressins/chemistry , Vasopressins/genetics
4.
J Neuroendocrinol ; 12(7): 685-93, 2000 Jul.
Article in English | MEDLINE | ID: mdl-10849214

ABSTRACT

Biosynthesis of the vasopressin (VP) prohormone in magnocellular neurones of the hypothalamo-neurohypophysial system comprises endoplasmic reticulum (ER) transit, sorting into the regulated secretory pathway and subsequent processing in the individual proteins VP, neurophysin and a glycoprotein. These processes are severely disrupted in the homozygous diabetes insipidus (di/di) Brattleboro rat, which expresses a mutant VP precursor due to a single nucleotide deletion in the neurophysin region of the VP gene resulting in VP deficiency. Previous studies have shown the presence of additional frameshift mutations in VP transcripts, in solitary magnocellular neurones of the di/di rat due to a GA dinucleotide deletion resulting in two different mutant VP precursors with partly restored reading frame. Frameshifted VP precursors are also expressed in several magnocellular neurones in wild-type rats. In this study, we determined if the +1 frameshifted precursors from di/di and wild-type rats can lead to biosynthesis of the hormone VP. Therefore, eukaryotic expression plasmids containing the frameshifted VP cDNAs were transiently expressed in peptidergic tumour cell lines, and cells were analysed by reversed phase high-performance liquid chromatography and specific radioimmunoassays, and by immunofluoresence. Neuro2A neuroblastoma cells expressing the +1 frameshifted precursors of di/di rats retained products in the cell body. Only precursor or insignificant quantities of neurophysin-immunoreactive products were detected. In contrast, in AtT20 cells, frameshifted VP precursors were at least partly processed to yield the VP peptide, indicating that they have access to the regulated secretory pathway. Comparison between the two cell lines showed a very slow ER transit of the wild-type prohormone combined with inefficient processing in Neuro2A cells. The results show that mutant precursors can reach the regulated secretory pathway if ER transport is sufficiently rapid as in the case of AtT20 cells. This suggests that the di/di rat may regain the capacity to biosynthesize authentic VP through these +1 frameshifted precursors in magnocellular neurones.


Subject(s)
Frameshift Mutation , Protein Precursors/genetics , Protein Processing, Post-Translational , Vasopressins/genetics , Animals , Biological Transport , Diabetes Insipidus/genetics , Endoplasmic Reticulum/metabolism , Gene Deletion , Mice , Protein Precursors/biosynthesis , Protein Precursors/metabolism , Rats , Rats, Brattleboro , Tissue Distribution , Tumor Cells, Cultured , Vasopressins/biosynthesis , Vasopressins/metabolism
5.
FEBS Lett ; 475(3): 175-80, 2000 Jun 23.
Article in English | MEDLINE | ID: mdl-10869551

ABSTRACT

The sorting of soluble proteins into the regulated secretory pathway (RSP) involves aggregation, but whether an additional sorting domain is also required is not clear. By fusing vasopressin prohormone (proVP) fragments to green fluorescent protein (eGFP) we have determined whether a sorting domain can function independently of the aggregative neurophysin domain. Although eGFP itself can be immunolocalised in the RSP of Neuro2A and AtT20 cells, most of the protein enters the constitutive pathway, and is found in the culture medium. In contrast, the N-terminal 27 residues of proVP promote residence in the RSP. Furthermore, only the processed form of this fusion was secreted when stimulated. We suggest a sorting mechanism based on the recognition of a sorting motif, the efficiency of which is enhanced by neurophysin aggregation.


Subject(s)
Vasopressins/metabolism , Animals , Biological Transport , Cell Line , Mice , Protein Precursors/metabolism
6.
J Neuroimmunol ; 106(1-2): 53-9, 2000 Jul 01.
Article in English | MEDLINE | ID: mdl-10814782

ABSTRACT

Endomorphin (EM)-1 and EM-2 are opioid tetrapeptides, reported within the central nervous system, which have very high specificity and affinity for the mu-opioid receptor. We have used newly developed and well-characterised radioimmunoassays (RIAs) in combination with reversed-phase high-performance liquid chromatography (HPLC) to detect EM-1 and EM-2 immunoreactivity (ir) in rat immune tissues. Endomorphins were detectable in extracts of rat spleen (total EM-1-ir/spleen: 440+/-73 pg, mean+/-SEM, a=group of eight rats; EM-2-ir: 150+/-12 pg) and thymus (EM-1-ir: 152+/-18 pg, mean+/-SEM n=8; EM-2-ir: 156+/-28 pg). EM-2-ir was detectable in extracts of human spleen (338+/-196 pg/g tissue, n=3). Multiple peaks of EM-1-ir and EM-2-ir were observed in rat spleen and thymus extracts, and multiple peaks of EM-2-ir were observed in extracts of human spleen, following reversed-phase HPLC and RIAs. This is the first report of endomorphin immunoreactivity in tissues of the rat and human immune systems.


Subject(s)
Immune System/chemistry , Oligopeptides/analysis , Adult , Animals , Chromatography, High Pressure Liquid , Cross Reactions , Female , Humans , Immune Sera/immunology , Male , Middle Aged , Radioimmunoassay/methods , Rats , Rats, Wistar , Spleen/chemistry , Thymus Gland/chemistry , Tissue Extracts/chemistry
7.
Protein Expr Purif ; 13(3): 319-25, 1998 Aug.
Article in English | MEDLINE | ID: mdl-9693056

ABSTRACT

Insulin-like growth factor-I (IGF-I), a single-chain polypeptide consisting of 70 amino acids and 3 disulfide bridges, is a member of a class of growth factors that are involved in many proliferative and metabolic processes. To assist in solving the crystallographic three-dimensional structure, we have expressed a recombinant fusion protein precursor of IGF-I in a methionine auxotrophic strain of Escherichia coli grown in the presence of selenomethionine. An homogeneous preparation of selenomethionyl-IGF-I was then obtained by chemical cleavage of the fusion protein. The selenomethionine analogue of IGF-I was characterized by electrospray mass spectrometry, peptide mapping, analytical chromatography, and electrophoresis as well as by biological assays. The final preparation of IGF-I was found to incorporate about 90% of selenium and fully retained the functional activity.


Subject(s)
Insulin-Like Growth Factor I/genetics , Selenomethionine/chemistry , Chromatography, High Pressure Liquid , Electrophoresis, Polyacrylamide Gel , Insulin-Like Growth Factor I/analogs & derivatives , Insulin-Like Growth Factor I/metabolism , Peptide Mapping , Radioligand Assay , Recombinant Proteins/chemistry , Recombinant Proteins/genetics , Recombinant Proteins/metabolism , Selenomethionine/metabolism , Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
8.
Cell Mol Neurobiol ; 18(2): 173-91, 1998 Apr.
Article in English | MEDLINE | ID: mdl-9535289

ABSTRACT

1. In this review the structure-function relationships of the different vasopressin prohormone domains are dated and discussed, with special reference to the neurophysin and glycopeptide domains. 2. The primary structures of the currently known neurophysins and glycopeptide sequences are compared and discussed. 3. The hormone-binding and aggregational properties of neurophysin are reviewed and related to a possible function within the regulated secretory pathway. 4. It is proposed, based on the properties reviewed here as well as our own data shown here, that the sorting of the vasopressin prohormone is initiated by hormone binding, which triggers aggregation of the prohormone into the characteristic dense cores of the regulated secretory pathway. 5. This may suggest that prohormone sorting into the regulated secretory pathway is, in general, determined by noncovalent, intramolecular interactions that promote aggregation.


Subject(s)
Protein Precursors/chemistry , Protein Precursors/physiology , Vasopressins/chemistry , Vasopressins/physiology , Amino Acid Sequence , Animals , Humans , Models, Molecular , Molecular Sequence Data , Protein Structure, Tertiary
9.
Adv Exp Med Biol ; 395: 9-21, 1995.
Article in English | MEDLINE | ID: mdl-8713948

ABSTRACT

In the oxytocin (OT) gene several regions can be discerned that have a function in regulating its expression. Firstly, in the proximal 5' flanking region regulatory elements have been discovered that are targets for transcription factors of the nuclear hormone receptor family. Through these elements the OT gene of rat and man is responsive to estrogens, thyroid hormones and retinoids. Furthermore, these elements can be employed by the nuclear hormone orphan receptor family for repressive or inductive actions. In the distal 5' flanking region the POU class III proteins Brn-1, Brn-2, Brn-4, that are expressed in magnocellular neurons, and Oct-6 are able to bind, but do not display a significant regulatory activity on the OT gene in heterologous expression systems. Secondly, the OT precursor harbours both the biologically active hormone and the protein neurophysin that is able to associate with the hormone. Heterologous expression of wild-type and mutant vasopressin cDNAs in peptidergic cell lines shows that the highly homologous vasopressin-associated neurophysin domain associates with the hormone domain within the prohormone. This intramolecular interaction between two prohormone domains serves an essential intracellular function, i.e. the proper sorting of the prohormone into the regulated secretory pathway.


Subject(s)
Oxytocin/genetics , Animals , Arginine Vasopressin/biosynthesis , Arginine Vasopressin/genetics , Gene Expression Regulation , Genes, Regulator , Humans , Neurophysins/metabolism , Octamer Transcription Factor-6 , Oxytocin/biosynthesis , Promoter Regions, Genetic , Rats , Receptors, Cytoplasmic and Nuclear/genetics , Transcription Factors/metabolism
10.
Biochimie ; 76(3-4): 315-9, 1994.
Article in English | MEDLINE | ID: mdl-7819342

ABSTRACT

To obtain a model for the sorting and processing of preprovasopressin (preproVP), rat VP cDNA was transfected in murine Neuro2A neuroblastoma cells, which do not express VP. The precursor of VP was expressed and processed into the authentic VP gene products VP, neurophysin (NP) and glycopeptide (GP) as determined with reversed phase HPLC and radioimmunoassay. In addition, Neuro2A-specific forms of NP and GP were observed, which may be produced in the constitutive secretory pathway in these cells.


Subject(s)
Gene Expression , Hypothalamus/metabolism , Pituitary Gland/metabolism , Protein Precursors/metabolism , Protein Processing, Post-Translational , Vasopressins/metabolism , Animals , Base Sequence , Cell Line , Chromatography, High Pressure Liquid , DNA Primers , Glycopeptides/analysis , Glycopeptides/biosynthesis , Male , Molecular Sequence Data , Neuroblastoma , Neurophysins/analysis , Neurophysins/biosynthesis , Polymerase Chain Reaction , Protein Precursors/biosynthesis , Radioimmunoassay , Rats , Rats, Wistar , Tumor Cells, Cultured , Vasopressins/analysis , Vasopressins/biosynthesis
12.
Peptides ; 14(4): 807-13, 1993.
Article in English | MEDLINE | ID: mdl-7993391

ABSTRACT

Previously it has been shown that vasopressin (VP) and oxytocin are converted by aminopeptidase activity in brain membranes into fragments with potent CNS activities. This report concerns the properties of this enzyme activity, addressed as VP-converting aminopeptidase (VP-AP) activity, in membranes of the rat brain. The VP-AP activity had a pH optimum at pH 7.0 and had a Km of 17 microM for its action on VP. Amastatin was the most potent aminopeptidase inhibitor. Enzyme activity was inhibited by relatively low concentrations of metal chelators. Treatment of brain membranes by EDTA resulted in loss of enzyme activity that was completely reversed by 10 microM Zn2+, indicating that VP-AP activity is a metallopeptidase. Several VP analogues and fragments, in particular VP(1-8), inhibited the action of enzyme activity on VP. Among peptides unrelated to VP, angiotension I, somatostatin, and porcine ACTH(1-39) markedly inhibited enzyme activity. Solubilization of VP-AP activity from brain membranes and gel filtration on Sephadex G200 showed two peaks of activity, one eluting with an apparent mass of about 140 kDa, the other in the void volume. Gel filtration fractions were able to convert [3H][Phe3]VP in a step-wise fashion. The VP-AP-like activity was found in many tissues outside the brain. Highest activity was present in lung, kidney, parts of the gastrointestinal tract, ovary, and uterus. The results indicate that VP-AP activity is a widely distributed enzyme with probably multiple functions, one of which involves the metabolism of vasopressin in the brain.


Subject(s)
Aminopeptidases/metabolism , Brain/metabolism , Vasopressins/metabolism , Animals , Kinetics , Male , Membranes/metabolism , Metals/pharmacology , Molecular Weight , Neuropeptides/pharmacology , Protease Inhibitors/pharmacology , Rats , Rats, Wistar , Solubility
SELECTION OF CITATIONS
SEARCH DETAIL
...