ABSTRACT
An indirect immunofluorescence staining technique was employed to evaluate the TQ1 and 5/9 monoclonal antibody lymphocyte reactivity in 10 cord blood mononuclear cell (MC) preparations enriched of E-rosette-forming cells (E+). Ten adult E+ MC populations were used as controls. Unfractionated T4+ cord and adult MC positively selected by panning procedure were also assayed. The results of these experiments, taken together, suggest that there is an overexpanded neonatal T cell subset which displays a previously unrecognized immunophenotype (T4+, TQ1+, 5/9+). Whether these lymphocytes are involved in the wellknown fetal-maternal immunosuppressive mechanisms of whether they are a further example of neonatal phenotypic immaturity remains to be elucidated.
Subject(s)
Fetal Blood/immunology , Infant, Newborn/immunology , T-Lymphocytes/immunology , Adult , Antibodies, Monoclonal/immunology , Fluorescent Antibody Technique , Humans , Infant, Newborn/blood , Middle Aged , Phenotype , Rosette FormationABSTRACT
A case of acute infectious lymphocytosis in an otherwise healthy 2-year-old child is reported. Marker analysis of the expanded blood lymphocytes showed that they were predominantly T cells and that there was a considerable increase in the helper/inducer phenotype (OKT4+) population. However, the lymphocyte response to polyclonal T-cell activators was low. This is the first report on T-cell subset distribution in acute infectious lymphocytosis.
Subject(s)
Lymphocytosis/genetics , T-Lymphocytes/immunology , Acute Disease , B-Lymphocytes/immunology , Child, Preschool , Female , Humans , Lymphocyte Activation , Phenotype , T-Lymphocytes, Helper-Inducer/immunologyABSTRACT
Plasma cell generation in pokeweed mitogen-pulsed lymphocyte cultures was markedly suppressed when the experiments were carried out by adding cell-depleted cerebrospinal fluid (CSF) from 5 acute aseptic meningitis or meningoencephalitis children known to have an overexpanded cell population with the suppressor-cytotoxic T-cell surface phenotype in their CSF. In contrast, B-cell terminal differentiation was not affected by the addition of CSF from 5 control children with non-neurological diseases, thereby indicating that CSF from central nervous system virus-infected children contains soluble factors which are themselves capable of exerting regulatory influences on immunocompetent cells.
Subject(s)
Lymphocytes/immunology , Meningitis, Aseptic/cerebrospinal fluid , Meningitis/cerebrospinal fluid , Meningoencephalitis/cerebrospinal fluid , Plasma Cells/immunology , Virus Diseases/cerebrospinal fluid , Adolescent , Cells, Cultured , Child , Female , Humans , Male , Meningitis, Aseptic/immunology , Meningoencephalitis/immunology , Virus Diseases/immunologySubject(s)
Antibodies, Monoclonal/immunology , Meningitis, Aseptic/blood , Meningitis/blood , Meningoencephalitis/blood , T-Lymphocytes/classification , Adolescent , Child , Female , Humans , Male , Meningitis, Aseptic/cerebrospinal fluid , Meningoencephalitis/cerebrospinal fluid , Phenotype , Surface PropertiesABSTRACT
A relative decrease in helper and a parallel increase in suppressor-cytotoxic T lymphocytes was found in the blood of six habitually aborting women, as compared with the T-cell subset distribution in ten normal multiparae and eight multigravid post-partum women. It is suggested that an imbalance between the immunoregulatory T-cell subpopulations may contribute to the rejection of the semiallogenic fetus.
