ABSTRACT
The authors report a modified T.L.C. - bioautograph method for detection of vitamin B12 active compounds in serum. This method offers particular advantages because it allows the identification and the quantitative assay of picograms of vitamin B12 active compounds.
Subject(s)
Vitamin B 12/blood , Chromatography, Thin Layer/methods , Humans , Microchemistry/methodsSubject(s)
Campylobacter Infections/diagnosis , Gastroenteritis/diagnosis , Agricultural Workers' Diseases/diagnosis , Agricultural Workers' Diseases/microbiology , Animals , Anti-Bacterial Agents/therapeutic use , Campylobacter Infections/microbiology , Campylobacter Infections/transmission , Campylobacter fetus/isolation & purification , Colon/pathology , Dog Diseases/transmission , Dogs , Food Handling , Foodborne Diseases/diagnosis , Gastroenteritis/microbiology , Humans , Occupational Diseases/diagnosis , Occupational Diseases/microbiology , Poultry , Poultry Diseases/transmission , PrognosisABSTRACT
From Oct. 30 to Nov. 7, 1979, 10 people in the Sardinian province of Cagliari had onset of bacteriologically confirmed cholera. Two symptom-free excretors of Vibrio cholerae O:1 were detected in household contacts of the patients. There were no deaths. All but 1 of the 12 people with V. cholerae O:1 infection gave a history of recent consumption of marine bivalves known locally as arselle (pelecypods). Triplicate matched neighbourhood controls for each of the first 7 cases identified were also interviewed; none had recently eaten arselle. V. cholerae O:1 was also recovered from samples of water and bivalves obtained from a lagoon on the outskirts of the city of Cagliari. Arselle had also been implicated as the vehicle of transmission in 1973 in the last outbreak of cholera in Sardinia. It seems unlikely that cholera transmission had persisted locally in the interim.
Subject(s)
Cholera/etiology , Disease Outbreaks/epidemiology , Mollusca/microbiology , Shellfish , Adolescent , Adult , Aged , Animals , Cholera/epidemiology , Disease Vectors/microbiology , Female , Foodborne Diseases/complications , Humans , Italy , Male , Middle Aged , Recurrence , Shellfish Poisoning , Vibrio cholerae/isolation & purificationABSTRACT
Herewith are the results of a research on determination of staphylococcal heat-stable nuclease in food. From this research derives clearly the need to devote to the determination of heat-stable deoxyribonuclease as an useful indirect index, fit to give menful informations on the pollution of different kind of food by the side of patogen Staphylococci even on food products under processes of pasteurization or of sterilization.
Subject(s)
Food Microbiology , Micrococcal Nuclease/analysisABSTRACT
The quantities are given of pyruvate in a medium to remark any possible connection between varieties of pyruvate and present bacterial flora concentration. We found some connection between the two experiments, even though, not always are very easily to menage.
Subject(s)
Bacteria/metabolism , Beverages/standards , Food Microbiology , Pyruvates/analysis , Food Analysis/methodsABSTRACT
The Authors report the results of comparative investigations carried out on some mineral waters bottled in various containers (laminated polyethylene, glass, PVC). The microbic charges vary in relation to the kind of container and resulted to be lower in the case of containers made of polyethylene.
Subject(s)
Food Preservation , Mineral Waters/standards , Water Microbiology , Glass , Italy , Mineral Waters/analysis , Polyethylenes , Polyvinyl ChlorideABSTRACT
Enriched cultures ofsing a selective substratum treated with sulfisoxazolo. This substratum has a composition in aminoacids, vitamins of the B-group, glucides and sodium chloride similar to that of homogenized Mytilus edulis which had turned out to be an excellent natural medium for the growth of the aforecited halofilic vibrios.
Subject(s)
Culture Media , Vibrio/isolation & purificationABSTRACT
In this paper a method is described to determine the bacteria count of samples by enumerating the microcolonies with the aid of a "Coulter Counter" automatic apparatus. Samples of bacterial cultures were incubated in a suitable medium at 32 degrees C for 12 h, then passed through a Coulter Counter aperture of 200 mu diameter to count the microcolonies. Microcolonies from cultures grown in similar conditions were also enumerated by the classical plate method. Comparison showed a good correction between the two sets of results after logarithmic transformation. Some advantages and limitations of this automatic technique are discussed, in view of its routine employment in food analysis.
