ABSTRACT
The objective of the study was to determine the presence of antibodies against Actinobacillus suis in pigs from technical breeding farms located in the northern, central and southern part of Lima. Blood samples of apparently healthy Actinobacillus suis-non-vaccinated pigs of 17-20 weeks of age were obtained from the local slaughterhouse (n=474). Serological evaluation was performed through a plate agglutination test with a specific antigen for Actinobaccillus suis, using three dilutions (1/40, 1/80 and 1/160). The volume of sera was 0.08, 0.04 and 0.01 ml, whereas the amount of antigen was kept constant (0.03 ml). Only those samples that agglutinated in all three dilutions were considered as positive reactors. The results indicated that 142 out of 474 sera tested showed antibodies against A. suis obtaining a prevalence of 30% with higher frequency of positives in the southern and northern part of Lima.
El presente estudio tuvo como objetivo determinar la presencia de anticuerpos contra Actinobaccilus suis en credos provenientes de granjas con crianza tecnificada del norte, centro y sur del departamento de Lima. Un total de 474 muestras de sangre de porcinos aparentemente sanos, no vacunados contra A. suis, de 17 a 20 semanas de edad se obtuvieron al momento de su beneficio. La evaluación serológica se realizó mediante la prueba de aglutinación en placa utilizando un antígeno específico contra A. suis empleando tres diluciones (1/40, 1/80 y 1/160) y una cantidad constante de antígeno (0.03 ml.) se consideraron positivos los sueros que aglutinaron en las tres diluciones. Se observó la presencia de reactores contra a. suis en 142 de las 474 muestras, determinándose una prevalencia del 30% con una mayor frecuencia de sueros positivos en las zonas sur y norte de Lima.