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1.
Fertil Steril ; 90(6): 2080-5, 2008 Dec.
Article in English | MEDLINE | ID: mdl-18249400

ABSTRACT

OBJECTIVE: To assess the level of lipid peroxidation (LP) and vitamin E in the follicular fluid and serum of infertile patients, with or without endometriosis, who were submitted to ovulation induction for assisted reproduction procedures. DESIGN: Prospective study. SETTING: Assisted conception unit, university hospital. PATIENT(S): Infertile patients 20 to 38 years of age were selected prospectively and consecutively and were divided into the endometriosis group (17 patients with pelvic endometriosis) and the control group (19 patients with previous tubal ligation or male factor and without endometriosis). INTERVENTION(S): Peripheral blood samples were collected on D1 (before the beginning of the use of gonadotropins), D2 (day of hCG administration), and D3 (day of oocyte retrieval). On D3, follicular-fluid samples free from blood contamination also were collected and stored. MAIN OUTCOME MEASURE(S): Lipid peroxidation was assessed by malondialdehyde quantification by spectrophotometry, and measurement of vitamin E was performed by HLPC. RESULT(S): On D1, no significant difference in LP was observed between groups. However, vitamin E levels were significantly higher in the control group. On D2, LP levels were significantly higher in the endometriosis group compared with in the control group, and vitamin E levels continued to be significantly higher in the control group. On D3, there was no significant difference in serum and follicular-fluid levels of LP and vitamin E between groups. However, on D3, vitamin E levels were found to be significantly higher in serum than in follicular fluid in both groups, whereas malondialdehyde levels were significantly lower in follicular fluid than in serum only in the control group. CONCLUSION(S): Before the beginning of ovulation induction, a significant decrease in vitamin E was observed in patients with endometriosis, perhaps because antioxidants are consumed during oxidation reactions. After ovulation induction with exogenous gonadotropins, the group of patients with endometriosis not only presented increased lipid peroxidation but also maintained lower vitamin E levels than the control group, a fact that hypothetically could compromise oocyte quality in endometriotic patients. However, on the day of oocyte retrieval, both serum LP potential and vitamin E levels were found to be similar in the two groups.


Subject(s)
Endometriosis/complications , Follicular Fluid/metabolism , Infertility, Female/therapy , Lipid Peroxidation , Ovulation Induction , Peritoneal Diseases/complications , Vitamin E/metabolism , Adult , Endometriosis/metabolism , Female , Humans , Infertility, Female/etiology , Infertility, Female/metabolism , Malondialdehyde/metabolism , Oocyte Retrieval , Peritoneal Diseases/metabolism , Pilot Projects , Prospective Studies , Time Factors , Vitamin E/blood , Young Adult
2.
Gynecol Endocrinol ; 22(8): 432-6, 2006 Aug.
Article in English | MEDLINE | ID: mdl-17012104

ABSTRACT

The aim of the present study was to measure the in vitro aromatase activity in granulosa cells of women with endometriosis submitted to assisted reproduction techniques. A case-control study was conducted on eight patients with endometriosis and eight with other infertility causes submitted to in vitro fertilization or intracytoplasmic sperm injection. Granulosa cells were obtained from pre-ovulatory follicles during oocyte retrieval and cultured for 24 h in the presence or absence of testosterone (2 x 10(-6) and 2 x 10(-5) M), follicle-stimulating hormone (FSH) and insulin-like growth factor-I (IGF-I) (both at 50 ng/ml). Estradiol (radioimmunoassay) was measured in the obtained culture fluids. The basal production of estradiol and its production under testosterone addition to the culture (aromatase activity) were analyzed. Reduced aromatase activity was detected in cultured granulosa cells in endometriosis cases, compared with controls, when testosterone was added at the concentration at 2 x 10(-6) M (p = 0.0303). The basal production of estradiol was also reduced in endometriosis patients (p = 0.0390). The effect of addition of FSH and IGF-I did not differ between groups. In conclusion, the in vitro basal production of estradiol and aromatase activity in granulosa cells were reduced in women with endometriosis submitted to assisted reproduction techniques, compared with the control group.


Subject(s)
Aromatase/metabolism , Endometriosis/enzymology , Granulosa Cells/enzymology , Reproductive Techniques, Assisted , Uterine Diseases/enzymology , Adult , Case-Control Studies , Cells, Cultured , Estradiol/biosynthesis , Female , Granulosa Cells/drug effects , Humans , Testosterone/pharmacology
3.
Contraception ; 71(4): 309-14, 2005 Apr.
Article in English | MEDLINE | ID: mdl-15792652

ABSTRACT

Sixteen fertile women aged 30-35 years with regular menstruations were studied before and 6 months after tubal ligation (TL). The diagnosis of ovulation was based on clinical ultrasonographic and hormonal parameters [follicle-stimulating hormone (FSH), luteinizing hormone (LH), E2 and P4]. The menstrual pattern did not vary before and after surgery. Follicular or luteal phase FSH and E2 levels did not differ between the pre- and post-TL period. Luteinizing hormone levels on days -2 and 0 of the follicular phase were significantly higher during the post-TL period, while no difference was observed for the luteal phase. P4 levels during the follicular phase did not differ between the two periods, except for day -4, while they were lower during the post-TL luteal phase. The results of the study suggest that in the present patient group, no modifications in the menstrual pattern could be observed 6 months after TL, and TL appears not to interfere with ovulation. Luteinizing hormone levels showed an increase during the ovulatory period after TL, and progesterone secretion decreased during the post-TL luteal phase.


Subject(s)
Hormones/blood , Menstrual Cycle/blood , Sterilization, Tubal , Adult , Case-Control Studies , Estradiol/blood , Female , Follicle Stimulating Hormone/blood , Humans , Luteinizing Hormone/blood , Progesterone/blood
4.
J Assist Reprod Genet ; 21(8): 311-4, 2004 Aug.
Article in English | MEDLINE | ID: mdl-15568333

ABSTRACT

PURPOSE: To assess the influence of ovarian endometrioma during IVF. METHODS: Patients were submitted to cystectomy by the laparoscopic route for exeresis of ovarian endometrioma. Group I (n = 28) consisted of patients without ovarian endometrioma and group II (n = 14) consisted of patients with recurrence of ovarian endometrioma during IVF. RESULTS: Fertilization and cleavage rates were higher in group I and the pregnancy rate per transfer was similar in the two groups. CONCLUSIONS: The presence of endometrioma during IVF causes a worsening of oocyte fertilization and embryo cleavage but does not affect the pregnancy rate per transfer.


Subject(s)
Endometriosis/complications , Fertilization in Vitro , Ovarian Diseases/complications , Pregnancy Rate , Adult , Cystectomy , Endometriosis/surgery , Female , Humans , Laparoscopy , Ovarian Diseases/surgery , Pregnancy
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