Deciphering regeneration through non-model animals: A century of experiments on cephalopod mollusks and an outlook at the future.

The advent of marine stations in the last quarter of the 19th Century has given biologists the possibility of observing and experimenting upon myriad marine organisms. Among them, cephalopod mollusks have attracted great attention from the onset, thanks to their remarkable adaptability to captivity and a great number of biologically unique features including a sophisticate behavioral repertoire, remarkable body patterning capacities under direct neural control and the complexity of nervous system rivalling vertebrates. Surprisingly, the capacity to regenerate tissues and complex structures, such as appendages, albeit been known for centuries, has been understudied over the decades. Here, we will first review the limited in number, but fundamental studies on the subject published between 1920 and 1970 and discuss what they added to our knowledge of regeneration as a biological phenomenon. We will also speculate on how these relate to their epistemic and disciplinary context, setting the base for the study of regeneration in the taxon. We will then frame the peripherality of cephalopods in regeneration studies in relation with their experimental accessibility, and in comparison, with established models, either simpler (such as planarians), or more promising in terms of translation (urodeles). Last, we will explore the potential and growing relevance of cephalopods as prospective models of regeneration today, in the light of the novel opportunities provided by technological and methodological advances, to reconsider old problems and explore new ones. The recent development of cutting-edge technologies made available for cephalopods, like genome editing, is allowing for a number of important findings and opening the way toward new promising avenues. The contribution offered by cephalopods will increase our knowledge on regenerative mechanisms through cross-species comparison and will lead to a better understanding of the complex cellular and molecular machinery involved, shedding a light on the common pathways but also on the novel strategies different taxa evolved to promote regeneration of tissues and organs. Through the dialogue between biological/experimental and historical/contextual perspectives, this article will stimulate a discussion around the changing relations between availability of animal models and their specificity, technical and methodological developments and scientific trends in contemporary biology and medicine.

E Pluribus Octo - Building Consensus on Standards of Care and Experimentation in Cephalopod Research; a Historical Outlook.

The Directive 2010/63/EU "on the protection of animals used for scientific purposes" originally induced some concern among cephalopod researchers, because of the inclusion of cephalopod mollusks as the only invertebrates among the protected species. Here we reflect on the challenges and issues raised by the Directive on cephalopod science, and discuss some of the arguments that elicited discussion within the scientific community, to facilitate the implementation of the Directive 2010/63/EU in the scientific research context. A short overview of the aims of the COST Action FA1301 "CephsInAction," serves as a paradigmatic instance of a pragmatic and progressive approach adopted to respond to novel legislative concerns through community-building and expansion of the historical horizon. Between 2013 and 2017, the COST Action FA1301 has functioned as a hub for consolidation of the cephalopod research community, including about 200 representatives from 21 countries (19 European). Among its aims, CephsInAction promoted the collection, rationalization, and diffusion of knowledge relevant to cephalopods. In the Supplementary Material to this work, we present the translation of the first-published systematic set of guidelines on the care, management and maintenance of cephalopods in captivity (Grimpe, 1928), as an example of the potential advantages deriving from the confluence of pressing scientific concerns and historical interests.

The Obvious in a Nutshell: Science, Medicine, Knowledge, and History.

The scope and mission of the history of science have been constant objects of reflection and debate within the profession. Recently, Lorraine Daston has called for a shift of focus: from the history of science to the history of knowledge. Such a move is an attempt at broadening the field and ridding it of the contradictions deriving from its modernist myth of origin and principle of demarcation. Taking the move from a pluralistic concept of medicine, the present paper explores the actual and possible contributions that a history of knowledge can offer to the history of medicine in particular. As we will argue, the history of medicine has always been a history of knowledge, but for good reasons has always stuck to the concept of medicine as its object and problem throughout the ages, including the modern, scientific one. We argue that, in the history of medicine, the demarcation between scientific and non-scientific represents an accident, but is not foundational as in the case of natural science. Furthermore, the history of medicine programmatically played a role in at least two academic domains (history proper and medical education), adjusting historical narratives of medical knowledge to its audience. Accordingly, we underscore that the history of both science and medicine, as traditionally defined, already provides room for almost the whole spectrum of approaches to history. Moreover, their different myths of origin can, and indeed must, be included in the reflexivity of the historical gaze. We argue that the position towards a history of science, medicine, or knowledge is not a question of narrative or theory, rather, it is a question of relevance and awareness of extant contexts.

One, no-one and a hundred thousand brains: J.C. Eccles, J.Z. Young and the establishment of the neurosciences (1930s-1960s).

Contemporary neurosciences have grown beyond the limits of a natural science. To its most vocal advocates, the study of the human brain can provide nothing short of the basis for a new science of man-the link between the "natural" and "human" sciences-as a simple consequence of the growing mass of facts relating to this most marvelous organ, accumulated in the last four decades. This straightforward picture of the growing import of the neurosciences simplifies and obscures the myriad different interpretations and images of "the brain" that have inspired the development of the neurosciences. Among them, this chapter will consider two deeply contrasting early images of the brain: the cellular-physiological brain proposed since the 1950s by John Carew Eccles, and the model-"whole" brain championed by John Zachary Young. Eccles' program was focused on the vertebrate synapse, and Young's on the whole brain of an "advanced" invertebrate (the octopus). The former was the programmatic extension of a long neurophysiological tradition, and the latter an outspoken attempt at providing a revolutionary model for the organization of an unprecedented research effort. One underscored continuity and scientific "soundness," and the other promised rupture and new, imaginative solutions to age-old problems. Nevertheless, they have been later lumped together into a single, marvelous and progressive history, or mythology, of the Science of the Brain. This chapter will show how the organizing principle of these two opposed (if almost equally successful) research efforts was not the foggy, ever-changing image of an experimental brain-in-becoming, but the clear, fixed horizon of a promised brain.

Clever Hans and his effects: Karl Krall and the origins of experimental parapsychology in Germany.

Shortly before the outbreak of World War I, the so-called Elberfeld horses, the counting and speaking animals, were among the most debated subjects of the newborn comparative psychology. Yet, they have left little trace in the historiography of this discipline, mostly as an appendix of the more famous Clever Hans. Their story is generally told as the prelude to the triumph of reductionistic experimental psychology. By paying a more scrupulous attention than has so far being done to the second life of Hans, and to the endeavours of his second master, Karl Krall, this article explores the story of the Elberfeld horses as an important, if so far neglected, chapter in the history of experimental parapsychology.

[An illustrious unknown. Giuseppe Levi among science, anti-fascism and Nobel Prizes]. / Uno sconosciuto illustre: Giuseppe Levi tra scienza, antifascismo e Premi Nobel.

The anatomist Giuseppe Levi (1872-1965) is unanimously considered one of the major figures of Italian biomedical sciences in the 20th century. His fame, however, is mainly derived from having nurtured three Nobel Prize winners, namely Salvador E. Luria, Rita Levi Montalcini and Renato Dulbecco. In reappraising Levi's role in the development of Italian science and culture in general, this article aims at questioning both the narrowness of earlier accounts and a certain kind of genealogical approach to the history of scientific disciplines and academic schools. We will here consider Giuseppe Levi as an instance of two major cultural phenomena: the development of experimental biology in Italy and continental Europe and the anti-fascist socialist culture expressed by a part of the Italian intellectuals. In so doing, we will reassess the historical specificity of the scientific maturation of Levi's three famous students, on the one hand, while on the other we will consider in some depth the cultural and moral environment in which Levi thrived and his role as a moral example for his students. Such revision, we will argue, have a direct bearing on more general historiographical issues, namely, the need for a stronger contextualization of the birth and consolidation of research traditions, implying a rejection of simplistic genealogical reconstructions, and the role of academic schools and institutional settings in the definition of novel, multidisciplinary scientific approaches. Finally, the following will highlight the importance of a more careful outlook on the master-pupil relationship in academic context, addressing issues of both continuity and rupture. The article is subdivided in two main sections, the first devoted to Levi as a scientist, the second to his Anti-fascism.

Southern genes. Genetics and its institutions in the Italian South, 1930s-1970s.

The paper examines the historical vicissitudes of Genetics and Medical Genetics in the "Mezzogiorno", focussing on the emergence of local traditions and their mingling with international trends. The development of these disciplines took place in a peculiar mix of politics and science that lead to a rapid growth in the '50s and the '60s, followed by an harsh crisis. Though important and enduring results were attained, Italian genetics community failed to maintain the status reached in the two preceding decades, and quickly moved to the periphery of international networks.

Nobel laureates at the Stazione Zoologica Anton Dohrn: phenomenology and paths to discovery in neuroscience.

The practice of science usually involves more than a solitary genius in a solitary room, coping with the problem of her/his life. From the second half of the 19th century onwards, scientific research, especially in the field of the Natural Sciences, has grown into a more and more complex practice, which often entangles very special needs, in terms of research objects, techniques, sources, and perspectives. A few special places, such as the Stazione Zoologica di Napoli, have represented in this period the focal points of an ever growing international scientific network, promoting independent research, exchange and diffusion of novel practices and techniques and unrestricted confrontation. The so-called "Naples experience" has been cited by a large number of renowned scientists of the last two centuries as a key moment in their scientific life. Here we have tried to test it against the experience of three great scientists par excellence, i.e. three Nobel laureates (T. H. Morgan, Otto Warburg, J. D. Watson). The different experiences they have had at Naples represent, in our view, three different moments of the professional life of almost every scientist. Therefore, we have chosen to present them as a phenomenology. The final section is dedicated to a survey of the Zoological Station's contribution to neurosciences, especially to the Naples experience of the Nobel Prize winner Sir Bernard Katz and his assistant Ricardo Miledi, between 1965 and 1970. Their work on the squid at Naples allowed probing and quantitative refinement of results already obtained on different animals and contributed to reinforce the long lasting neurophysiological tradition of the institute.

[Genetics and international cooperation. Giuseppe Montalenti and modern biology]. / Genetica e cooperazione internazionale. Il con- tributo di Giuseppe Montalenti alla biologia contemporanea.

Giuseppe Montalenti (1904-1991) belongs to the generation of scientists that--through scientific and political efforts--have contributed to the rebuilding of Italian and European science after WWII. In this essay I will briefly encompass his contribution to the science of Genetics as a researcher, as an academic and as a science administrator, especially focusing on his valuable contributions to the field of Human Population Genetics in the 1950s and 1960s and on his role in the promotion of projects of international cooperation (viz. the International Biological Program of the ICSU, 1965-1974).