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Anal Chim Acta ; 1309: 342666, 2024 Jun 22.
Article in English | MEDLINE | ID: mdl-38772654

ABSTRACT

BACKGROUND: Peroxisome proliferator-activated receptors (PPARs) belong to the superfamily of nuclear receptors and represent the targets for the therapeutical treatment of type 2 diabetes, dyslipidemia and hyperglycemia associated with metabolic syndrome. Some medicinal plants have been traditionally used to treat this kind of metabolic diseases. Today only few drugs targeting PPARs have been approved and for this reason, the rapid identification of novel ligands and/or chemical scaffolds starting from natural extracts would benefit of a selective affinity ligand fishing assay. RESULTS: In this paper we describe the development of a new ligand fishing assay based on size exclusion chromatography (SEC) coupled to LC-MS for the analysis of complex samples such as botanical extracts. The known PPARα and PPARγ ligands, WY-14643 and rosiglitazone respectively, were used for system development and evaluation. The system has found application on an Allium lusitanicum methanolic extract, containing saponins, a class of chemical compounds which have attracted interest as PPARs ligands because of their hypolipidemic and insulin-like properties. SIGNIFICANCE: A new SEC-AS-MS method has been developed for the affinity screening of PPARα and PPARγ ligands. The system proved to be highly specific and will be used to improve the throughput for the identification of new selective metabolites from natural souces targeting PPARα and PPARγ.


Subject(s)
Chromatography, Gel , PPAR alpha , PPAR gamma , Plant Extracts , PPAR gamma/metabolism , PPAR gamma/chemistry , PPAR alpha/metabolism , Plant Extracts/chemistry , Plant Extracts/pharmacology , Ligands , Mass Spectrometry , Rosiglitazone/pharmacology , Rosiglitazone/chemistry , Humans , Biological Products/chemistry , Biological Products/pharmacology , Biological Products/analysis , Pyrimidines
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