ABSTRACT
BACKGROUND: Urine monitoring programs represent an important strategy for early diagnosis of reactivation of BK polyomavirus (BKV) in kidney transplant recipients. This study analyzes a BKV urine screening model in kidney transplant patients. METHODS: Urinary screening for BKV reactivation was performed by urinary decoy cell and polymerase chain reaction (PCR) tests in samples from 32 consecutive kidney transplant patients, collected in a 6-month follow-up period. PCR in plasma samples and BKV immunohistochemical studies to assess BKV renal disease, if a kidney biopsy was indicated, were performed. RESULTS: The urinary screening for BKV among 32 renal receptors was positive in 18 patients (56%) by the concomitant use of the decoy cells and/or qualitative PCR at some time during the study period. Transfusion before transplantation was significantly associated with urinary decoy cell positive screening (odds ratio = 11; 95% confidence interval = 1.47 to 82.4; P < .05); and so was male sex (odds ratio = 2.02; 95% confidence interval = 1.07 to 3.83; P < .05). The clinical management of screening positive cases consisted of decreasing or changing the immunosuppression regimen. Sixteen renal biopsies were performed. Immunohistochemistry for SV40 T antigen was negative in all biopsies. After 1 year of follow-up, no patient developed BKV-associated nephropathy, and there was no difference in renal function between patients positive and negative for BKV urinary screening. CONCLUSIONS: Early urinary monitoring is effective in detection of BKV replication and represents a good strategy to minimize the deleterious effects caused by the presence of the virus on preservation of graft function.
Subject(s)
DNA, Viral/urine , Kidney Diseases/urine , Kidney Transplantation , Polyomavirus Infections/urine , Tumor Virus Infections/urine , Adult , BK Virus/genetics , Biopsy , Female , Graft Rejection/prevention & control , Humans , Immunohistochemistry , Immunosuppressive Agents/adverse effects , Kidney Diseases/diagnosis , Kidney Diseases/etiology , Kidney Diseases/pathology , Male , Mass Screening , Middle Aged , Odds Ratio , Pilot Projects , Polymerase Chain Reaction , Polyomavirus Infections/diagnosis , Polyomavirus Infections/etiology , Sex Factors , Transplant Recipients , Tumor Virus Infections/diagnosis , Tumor Virus Infections/etiology , UrinalysisSubject(s)
Arthrogryposis/genetics , Cholestasis, Intrahepatic/genetics , Kidney Diseases/genetics , Mutation/genetics , Nuchal Translucency Measurement , Vesicular Transport Proteins/genetics , Arthrogryposis/diagnostic imaging , Cholestasis, Intrahepatic/diagnostic imaging , Homozygote , Humans , Infant , Kidney Diseases/diagnostic imaging , Kidney Tubules , Male , Portugal/ethnology , SyndromeSubject(s)
Coloboma/genetics , Kidney/abnormalities , Optic Nerve/abnormalities , Adolescent , Adult , Aged , Brazil , Child , Child, Preschool , DNA Mutational Analysis , DNA-Binding Proteins/genetics , Female , Humans , Male , PAX2 Transcription Factor , Pedigree , Peritoneal Dialysis , Point Mutation , Polymerase Chain Reaction , Renal Insufficiency/etiology , Renal Insufficiency/therapy , Syndrome , Transcription Factors/geneticsABSTRACT
The neuronal ceroid lipofuscinoses (NCL) are a group of inherited progressive neurodegenerative disorders with presentation from infancy to adulthood. Three main childhood forms can be established on the basis of age of onset, clinical course, and ultrastructural morphology: infantile (INCL), late infantile (LINCL), and juvenile (JNCL). Several variant subtypes have been described. Genetic and biochemical analysis are helping to better understand, diagnose and classify these disorders. We report on clinical, neurophysiological, neuroradiological, and morphological data from 17 patients with different forms (infantile, late infantile, and juvenile ) of neuronal ceroid lipofuscinoses (NCL) evaluated at Hospital de Clínicas de Porto Alegre, Southern Brazil, during 6 years (1992-1997). Seven cases were infantile; 5 were late infantile; and 5 were juvenile NCL. Gender ratio was male:female, 11:6. Age at presentation varied from 2-24 months for INCL; 2,5 to 5 years for LINCL; and 4-10 years for the JNCL cases. Seizures (6 patients) and psychomotor retardation (1 patient) were the initial symptoms in the INCL group. All the patients in the group of LINCL had the usual findings. JNCL patients manifested different initial symptoms, although tending to follow a similar clinical picture within familial cases. Epidemiological data on the prevalence of NCLs in Brazil are not available, we expect this series of cases to contribute to further research in our population.
Subject(s)
Neuronal Ceroid-Lipofuscinoses/complications , Adolescent , Adult , Age Factors , Age of Onset , Brazil , Child , Child, Preschool , Female , Humans , Infant , Male , Middle Aged , Neuronal Ceroid-Lipofuscinoses/genetics , Neuronal Ceroid-Lipofuscinoses/pathology , PedigreeABSTRACT
The objective of this prospective multicenter study was to determine whether cisapride is associated with increased risk of malformations, spontaneous abortions, or decreased birthweight when used during pregnancy. Cases were paired for age, smoking, and alcohol consumption with controls exposed to nonteratogens, as well as with disease-paired controls. One hundred and twenty-nine pregnant women were exposed to cisapride during pregnancy, including 88 during the period of fetal organogenesis. There were no differences in maternal history, birthweight, gestational age at delivery, and rates of livebirths, spontaneous or therapeutic abortions, fetal distress, and major or minor malformations among groups. It is concluded that exposure to cisapride during pregnancy is not associated with a major increased risk of malformations or spontaneous abortions or with decreased birthweight.
