Bacterial adhesion inhibitor prevents infection in a rodent surgical incision model.

Surgical site infection risk continues to increase due to lack of efficacy in current standard of care drugs. New methods to treat or prevent antibiotic-resistant bacterial infections are needed. Multivalent Adhesion Molecules (MAM) are bacterial adhesins required for virulence. We developed a bacterial adhesion inhibitor using recombinant MAM fragment bound to polymer scaffold, mimicking MAM7 display on the bacterial surface. Here, we test MAM7 inhibitor efficacy to prevent Gram-positive and Gram-negative infections. Using a rodent model of surgical infection, incision sites were infected with antibiotic-resistant bioluminescent strains of Staphylococcus aureus or Pseudomonas aeruginosa. Infections were treated with MAM7 inhibitor or control suspension. Bacterial abundance was quantified for nine days post infection. Inflammatory responses and histology were characterized using fixed tissue sections. MAM7 inhibitor treatment decreased burden of S. aureus and P. aeruginosa below detection threshold. Bacterial load of groups treated with control were significantly higher than MAM7 inhibitor-treated groups. Treatment with inhibitor reduced colonization of clinically-relevant pathogens in an in vivo model of surgical infection. Use of MAM7 inhibitor to block initial adhesion of bacteria to tissue in surgical incisions may reduce infection rates, presenting a strategy to mitigate overuse of antibiotics to prevent surgical site infections.

Adherence of pregnant women to dietary counseling and adequacy of total gestational weight gain.

INTRODUCTION: in scientific literature there are not published researches which have used a validated assessment method of adherence to dietary counseling capable of being reproduced. OBJECTIVE: to evaluate the adherence of pregnant women to dietary counseling during the prenatal period using a validated instrument according to the outcome of total gestational weight gain. METHODS: two hundred and eight adult pregnant women participated in the research. The instrument for adherence classification to dietary counseling was elaborated according to four evaluation criteria: food quantity and quality, meal pattern and adjustment to weekly weight gain. We classified as poor adherence when zero-one criterion was observed, good adherence when 2-3 aspects were observed and excellent when the amount was 4 aspects. RESULTS: Pregnant women with good or excellent adherence had a higher adjustment proportion of the total gestational weight gain in comparison to those who presented poor adherence in the second and fourth visits, respectively (p < 0.006; p < 0.007). Women with poor adherence to dietary counseling in the second visit presented about three times more chances of having poor adherence in the fourth visit (OR = 3.11; CI = 1.46-6.36). CONCLUSION: Adherence of pregnant women to dietary counseling had a positive association with the adjustment of total gestational weight gain.