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1.
Mol Cell Neurosci ; 37(3): 519-27, 2008 Mar.
Article in English | MEDLINE | ID: mdl-18191580

ABSTRACT

Peripheral myelin formation depends on axonal signals that tightly control proliferation and differentiation of the associated Schwann cells. Here we demonstrate that the molecular program controlling proliferation of Schwann cells switches at birth. We have analyzed the requirements for three members of the cyclin-dependent kinase (cdk) family in Schwann cells using cdk-deficient mice. Mice lacking cdk4 showed a drastic decrease in the proliferation rate of Schwann cells at postnatal days 2 and 5, but proliferation was unaffected at embryonic day 18. In contrast, ablation of cdk2 and cdk6 had no significant influence on postnatal Schwann cell proliferation. Taken together, these findings indicate that postnatal Schwann cell proliferation is uniquely controlled by cdk4. Despite the lack of the postnatal wave of Schwann cell proliferation, axons were normally myelinated in adult cdk4-deficient sciatic nerves. Following nerve injury, Schwann cells lacking cdk4 were unable to re-enter the cell cycle, while Schwann cells deficient in cdk2 or cdk6 displayed proliferation rates comparable to controls. We did not observe compensatory effects such as elevated cdk4 levels in uninjured or injured nerves of cdk2 or cdk6-deficient mice. Our data demonstrate that prenatal and postnatal Schwann cell proliferation are driven by distinct molecular cues, and that postnatal proliferation is not a prerequisite for the generation of Schwann cell numbers adequate for correct myelination.


Subject(s)
Cell Proliferation , Cyclin-Dependent Kinase 4/metabolism , Gene Expression Regulation, Developmental/physiology , Myelin Sheath/metabolism , Schwann Cells/physiology , Sciatic Neuropathy/enzymology , Animals , Animals, Newborn , Bromodeoxyuridine/metabolism , Cell Cycle/physiology , Cells, Cultured , Cyclin-Dependent Kinase 2/deficiency , Cyclin-Dependent Kinase 4/deficiency , Cyclin-Dependent Kinase 6/deficiency , Embryo, Mammalian , Gene Expression Regulation, Developmental/genetics , Ki-67 Antigen/metabolism , Mice , Mice, Knockout , Rats , Wallerian Degeneration/metabolism
2.
Glia ; 53(2): 147-57, 2006 Jan 15.
Article in English | MEDLINE | ID: mdl-16206162

ABSTRACT

Regulated cell proliferation is a crucial prerequisite for Schwann cells to achieve myelination in development and regeneration. In the present study, we have investigated the function of the cell cycle inhibitors p21 and p16 as potential regulators of Schwann cell proliferation, using p21- or p16-deficient mice. We report that both inhibitors are required for proper withdrawal of Schwann cells from the cell cycle during development and following injury. Postnatal Schwann cells express p21 exclusively in the cytoplasm, first detectable at postnatal day 7. This cytoplasmic p21 expression is necessary for proper Schwann cell proliferation control in the late development of peripheral nerves. After axonal damage, p21 is found in Schwann cell nuclei during the initiation of the proliferation period. This stage is critically regulated by p21, since loss of p21 leads to a strong increase in Schwann cell proliferation. Unexpectedly, p21 levels are upregulated in this phase suggesting that the role of p21 may be more complex than purely inhibitory for the Schwann cell cycle. However, inhibition of Schwann cell proliferation is the overriding crucial function of p21 and p16 in peripheral nerves as revealed by the consequences of loss-of-function in development and after injury. Different mechanisms appear to underlie the inhibitory function, depending on whether p21 is cytoplasmic or nuclear.


Subject(s)
Cyclin-Dependent Kinase Inhibitor p16/physiology , Cyclin-Dependent Kinase Inhibitor p21/physiology , Schwann Cells/drug effects , Adenoviridae/genetics , Animals , Blotting, Western , Cell Nucleus/drug effects , Cell Nucleus/metabolism , Cell Proliferation/drug effects , Fluorescent Antibody Technique , Genetic Vectors , Immunoenzyme Techniques , Keratinocytes/drug effects , Keratinocytes/metabolism , Mice , Nerve Fibers/physiology , Rats , Reverse Transcriptase Polymerase Chain Reaction , Sciatic Nerve/injuries , Skin/injuries , Skin/pathology , Transfection , Wound Healing
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