ABSTRACT
Dental calculus is a microbial biofilm that contains biomolecules from oral commensals and pathogens, including those potentially related to cause of death (CoD). To assess the utility of calculus as a diagnostically informative substrate, in conjunction with paleopathological analysis, calculus samples from 39 individuals in the Smithsonian Institution's Robert J. Terry Collection with CoDs of either syphilis or tuberculosis were assessed via shotgun metagenomic sequencing for the presence of Treponema pallidum subsp. pallidum and Mycobacterium tuberculosis complex (MTBC) DNA. Paleopathological analysis revealed that frequencies of skeletal lesions associated with these diseases were partially inconsistent with diagnostic criteria. Although recovery of T. p. pallidum DNA from individuals with a syphilis CoD was elusive, MTBC DNA was identified in at least one individual with a tuberculosis CoD. The authenticity of MTBC DNA was confirmed using targeted quantitative PCR assays, MTBC genome enrichment, and in silico bioinformatic analyses; however, the lineage of the MTBC strain present could not be determined. Overall, our study highlights the utility of dental calculus for molecular detection of tuberculosis in the archaeological record and underscores the effect of museum preparation techniques and extensive handling on pathogen DNA preservation in skeletal collections.
Subject(s)
Dental Calculus , Metagenomics , Mycobacterium tuberculosis , Paleopathology , Tuberculosis , Dental Calculus/microbiology , Dental Calculus/history , Humans , Metagenomics/methods , Paleopathology/methods , Tuberculosis/diagnosis , Tuberculosis/microbiology , Mycobacterium tuberculosis/genetics , Mycobacterium tuberculosis/isolation & purification , DNA, Bacterial/genetics , Male , Treponema pallidum/genetics , Treponema pallidum/isolation & purification , Syphilis/diagnosis , Syphilis/microbiology , Syphilis/history , Female , Adult , Metagenome/genetics , Middle AgedABSTRACT
Forensic anthropology casework frequently encounters evidence of animal scavenging associated with fragmentation and loss of skeletal material. Published research demonstrates that patterns of destruction in bone can suggest the size and type of animal involved. This study analyzes 107 cases reported on by the first author at the Smithsonian Institution to investigate patterns of scavenging in forensic anthropology casework. This investigation reveals that the extent of scavenger impact varies across the body, but primarily is concentrated in the central body area. Although extensive animal scavenging can limit analysis, some evidence of foul play can be preserved.
ABSTRACT
Research has documented considerable population variation in sexual dimorphism related to human growth and development. This variation represents both genetic and environmental factors which impact methodologies used to estimate sex from human skeletal remains. This article provides an overview of known variation in skeletal sexual dimorphism among populations through documented research on samples from around the world. Variation in juvenile growth patterns of populations and differences in adult skeletal size and characteristics are discussed. This recognized variation should be considered by forensic anthropologists when estimating sex from skeletal remains and appropriate population-specific data should be utilized.
