ABSTRACT
In this study, we report the genome sequence of five isolates of strawberry mottle virus (family Secoviridae, order Picornavirales) from strawberry field samples with decline symptoms collected in Eastern Canada. The Canadian isolates differed from the previously characterized European isolate 1134 in that they had a longer RNA2, resulting in a 239-amino-acid extension of the C-terminal region of the polyprotein. Sequence analysis suggests that reassortment and recombination occurred among the isolates. Phylogenetic analysis revealed that the Canadian isolates are diverse, grouping in two separate branches along with isolates from Europe and the Americas.
Subject(s)
Fragaria/virology , Plant Diseases/virology , Plant Viruses/genetics , RNA Viruses/genetics , Canada , Europe , Genetic Variation , Genome, Viral , Phylogeny , Plant Viruses/classification , Plant Viruses/isolation & purification , RNA Viruses/classification , RNA Viruses/isolation & purification , RNA, Viral/genetics , Reassortant Viruses/genetics , Reassortant Viruses/isolation & purification , Recombination, GeneticABSTRACT
Strawberry decline disease, probably caused by synergistic reactions of mixed virus infections, threatens the North American strawberry industry. Deep sequencing of strawberry plant samples from eastern Canada resulted in the identification of a new virus genome resembling poleroviruses in sequence and genome structure. Phylogenetic analysis suggests that it is a new member of the genus Polerovirus, family Luteoviridae. The virus is tentatively named "strawberry polerovirus 1" (SPV1).
