Detection of long chain per- and polyfluoroalkyl substances (PFAS) in the benthic Golden tilefish (Lopholatilus chamaeleonticeps) and their association with microscopic hepatic changes.

Oceans are major sinks for anthropogenic pollutants, including per- and polyfluoroalkyl substances (PFAS). Although PFAS have been detected in surface waters globally, this is the first report of PFAS in a deep (170-400 m) demersal species in the Gulf of Mexico (GoM). Golden Tilefish (Lopholatilus chamaeleonticeps) plasma extracts (n = 185) were investigated for the presence of PFAS using ultra-high performance liquid chromatography-tandem mass spectrometry. A subset of liver tissues (n = 51) were also analyzed for microscopic hepatic changes (MHCs). Overall, nine of the 110 PFAS targeted were detected in Tilefish plasma at relatively high frequencies. Plasma concentrations of total PFAS (Σ9PFAS) ranged from below the detection limit to 27.9 ng g-1 w.w. Significant regional differences were observed with the highest concentrations of PFAS detected in the north central region of the GoM, where substantial industrialization and discharges from the Mississippi River occur. Compared to most wildlife and matrices analyzed globally, the PFAS profiles in Tilefish were unique as they are dominated by PFUnDA. Profile differences are hypothesized to be the result of Tilefish's distinctive lifestyle, habitat, diet, and partitioning characteristics of long-chain PFAS. Several MHCs were identified in this subset of Tilefish that could be detrimental to their health. Significant correlations between PFAS concentrations and biometric indices and MHCs were evident, however, additional research is needed to investigate the role PFAS and PFAS combined with chemical admixtures may play in inducing observed hepatic changes and other physiological effects in Tilefish. These findings give insight into the fate of PFAS at depth in aquatic ecosystems and are cause for concern regarding the health of other deep water benthic biota in GoM and other deepwater sinks for PFAS.

A review of the toxicology of oil in vertebrates: what we have learned following the Deepwater Horizon oil spill.

In the wake of the Deepwater Horizon (DWH) oil spill, a number of government agencies, academic institutions, consultants, and nonprofit organizations conducted lab- and field-based research to understand the toxic effects of the oil. Lab testing was performed with a variety of fish, birds, turtles, and vertebrate cell lines (as well as invertebrates); field biologists conducted observations on fish, birds, turtles, and marine mammals; and epidemiologists carried out observational studies in humans. Eight years after the spill, scientists and resource managers held a workshop to summarize the similarities and differences in the effects of DWH oil on vertebrate taxa and to identify remaining gaps in our understanding of oil toxicity in wildlife and humans, building upon the cross-taxonomic synthesis initiated during the Natural Resource Damage Assessment. Across the studies, consistency was found in the types of toxic response observed in the different organisms. Impairment of stress responses and adrenal gland function, cardiotoxicity, immune system dysfunction, disruption of blood cells and their function, effects on locomotion, and oxidative damage were observed across taxa. This consistency suggests conservation in the mechanisms of action and disease pathogenesis. From a toxicological perspective, a logical progression of impacts was noted: from molecular and cellular effects that manifest as organ dysfunction, to systemic effects that compromise fitness, growth, reproductive potential, and survival. From a clinical perspective, adverse health effects from DWH oil spill exposure formed a suite of signs/symptomatic responses that at the highest doses/concentrations resulted in multi-organ system failure.

Generation of red drum (Sciaenops ocellatus) hematology Reference Intervals with a focus on identified outliers.

BACKGROUND: The Deepwater Horizon (DWH) oil spill in 2010 released millions of barrels of crude oil into the northern Gulf of Mexico, exposing numerous species of animals to the toxic components of oil. A comprehensive assessment of morbidity and mortality caused by DWH oil exposure was undertaken by the DWH Natural Resource Damage Trustees to characterize ecosystem damages. OBJECTIVES: This study aimed to characterize normal hematologic RIs in red drum fish with blood cell descriptions, and to demonstrate the importance of identifying and removing outliers when generating RI. METHODS: Two years after the oil spill, 57 adult, red drum fish of mixed sexes were caught along the eastern Louisiana coastline. Eight different sites were chosen to catch the fish; 6 sites were contaminated with oil, and 2 sites were not contaminated at the time of the oil spill. Hematologic RIs were generated from heparinized whole blood samples of healthy red drum as determined by gross examination and histopathologic examination. Two methods were used to detect hematologic effects likely caused by oil contamination. RESULTS: Red drum PCVs (RI 42-62%) were higher than previously reported in cold water and bottom-dwelling fish species, while absolute WBC counts (RI 2.9-8.7  × 109 /L) were comparable to WBC counts previously reported in other fish species with heterophil and lymphocyte absolute concentrations frequently being equivalent. Anemic animals (PCV<42%) were only identified in oil-contaminated sites. CONCLUSION: RIs in many wild fish species are lacking, and therefore, this study provides valuable baseline data on healthy red drum fish. The outliers assessed using ASVCP RI guidelines can provide valuable clinical information regarding individuals in population health assessments, which may be more sensitive for the detection of abnormalities than for population statistics comparing the mean. The importance of removing outliers and rerunning RI statistics is highlighted by this field example.

Developmental exposure to valproic acid alters the expression of microRNAs involved in neurodevelopment in zebrafish.

Congenital malformations are a prevalent cause of infant mortality in the United States and their induction has been linked to a variety of factors, including exposure to teratogens. However, the molecular mechanisms of teratogenicity are not fully understood. MicroRNAs are an important group of small, non-coding RNAs that regulate mRNA expression. MicroRNA roles in early embryonic development are well established, and their disruption during development can cause abnormalities. We hypothesized that developmental exposure to teratogens such as valproic acid alters microRNA expression profiles in developing embryos. Valproic acid is an anticonvulsant and mood-stabilizing drug used to treat epilepsy, bipolar disorder and migraines. To examine the effects of valproic acid on microRNA expression during development, we used zebrafish embryos as a model vertebrate developmental system. Zebrafish embryos were continuously exposed to valproic acid (1mM) or vehicle control (ethanol) starting from 4h post-fertilization (hpf) and sampled at 48 and 96hpf to determine the miRNA expression profiles prior to and after the onset of developmental defects. At 96hpf, 95% of the larvae showed skeletal deformities, abnormal swimming behavior, and pericardial effusion. Microarray expression profiling was done using Agilent zebrafish miRNA microarrays. Microarray results revealed changes in miRNA expression at both time points. Thirteen miRNAs were differentially expressed at 48hpf and 22 miRNAs were altered at 96hpf. Among them, six miRNAs (miR-16a, 18c, 122, 132, 457b, and 724) were common to both time points. Bioinformatic target prediction and examination of published literature revealed that these miRNAs target several genes involved in the normal functioning of the central nervous system. These results suggest that the teratogenic effects of valproic acid could involve altered miRNA expression.