Inhalable dust as a marker of exposure to airborne biological agents in composting facilities.

OBJECTIVES: Industrial composting is associated with high levels of worker exposure to bioaerosols. Measurement of airborne microorganisms and endotoxin is complex and the related cost is high. The objective was therefore to examine whether dust measurement could be used as a marker of exposure to bioaerosols in composting facilities. METHODS: A dataset of 110 measurements carried out in eight sludge composting plants was explored. Mixed-effects models were constructed to explain between-site and within-site variability in concentration of endotoxin and culturable mesophilic bacteria, mesophilic moulds and thermophilic actinomycetes in air. Fixed-effects variables were inhalable dust concentration, the season, the outdoor/indoor location of sampling and the process area. RESULTS: The level of dust was a highly significant determinant of concentration for all biological agents. Within-site variability was always larger than between-site variability. The proportion of within-site variability explained by determinants was 68%, 65%, 56% and 60% for endotoxin, bacteria, moulds and actinomycetes, respectively. Inclusion of dust in the final model resulted in an increase of 24, 20, 12 and 17 points of percentage within-site variability, respectively. Inclusion of season resulted in an increase of 9, 12, 12 and 15 points, respectively. Within-site variability was less influenced by outdoor/indoor location and process area, except for moulds. CONCLUSION: Dust was the factor that most influenced within-site variability in endotoxin and culturable bacteria concentration. Measurement of dust can efficiently assist decision making for prevention measures against endotoxin and bacteria in sludge composting plants. Our results are not as conclusive for actinomycetes and especially for moulds.

Aspergillus fumigatus and mesophilic moulds in air in the surrounding environment downwind of non-hazardous waste landfill sites.

Non-hazardous waste landfilling has the potential to release biological agents into the air, notably mould spores. Some species, such as Aspergillus fumigatus, may be a cause of concern for at-risk nearby residents. However, air concentration in the surrounding environment of non-hazardous waste landfill sites is poorly documented. An extensive sampling programme was designed to investigate the relationship between culturable mesophilic moulds and A. fumigatus concentrations in air and distance downwind of non-hazardous waste landfill sites. On-site and off-site repeated measurements were performed at four landfill sites during cold and warm seasons. A high-flow air-sampler device was selected so as to allow peak concentration measurement. Linear mixed-effects models were used to explain variability in the concentrations in air over time and across sites, seasons, instantaneous meteorological conditions and discharged waste tonnage. Concentrations of mesophilic moulds and A. fumigatus at off-site upwind sampling locations were compared with concentrations at each of the downwind sampling locations. At the tipping face location, peak concentration reached 480,000CFUm(-3) for mesophilic moulds and 9300CFUm(-3) for A. fumigatus. Compared with upwind background levels, these concentrations were, on average, approximately 20 and 40 times higher respectively. A steep decline in the concentration of both mesophilic moulds and A. fumigatus was observed between the tipping face location and the downwind property boundary (reduction by 77% and 84% respectively), followed by a low decline leading to a 90% and 94% reduction in concentration at 200m from the property boundary and beyond. With the 200m and 500m downwind sampling point values added together, the 97.5th percentile of concentration was 6013CFUm(-3) and 87CFUm(-3) for mesophilic moulds and A. fumigatus, respectively. Other determining factors were the discharged waste tonnage, the season, instantaneous temperature and wind velocity for mesophilic mould, and instantaneous temperature for A. fumigatus. At 200m and 500 downwind from the property boundary, mesophilic moulds and A. fumigatus concentrations were still higher than the local background level. However, whilst statistically significant, this increase does not suggest an excess risk to nearby residents' health when compared with the wide range of outdoor background levels reported in literature. These findings suggest that moulds and A. fumigatus may be transported beyond 200m from the property boundary in concentrations above those found locally upwind of the landfill site. Nevertheless, for exposure assessment purposes, comparison should also be made with background levels in wider areas which are either residential or through which people travel to work for example.