ABSTRACT
The addition of butyric acid to murine erythroleukemic cells (clone T3Cl2) induced the cells to differentiate, producing adult hemoglobin (A, alpha 2,beta 2) and an embryonic hemoglobin (E2, alpha 2Y2). The subsequent addition of hemin to the differentiating cells increased the synthesis of adult hemoglobin four-fold and the synthesis of embryonic hemoglobin two-fold; the relative synthesis of the alpha and beta globins increased more than the y globin. The embryonic hemoglobin was expressed prior to the adult hemoglobin in differentiating cells.
Subject(s)
Heme/analogs & derivatives , Hemin/pharmacology , Hemoglobins/biosynthesis , Leukemia, Erythroblastic, Acute/metabolism , Leukemia, Experimental/metabolism , Animals , Butyrates/pharmacology , Butyric Acid , Cell Differentiation/drug effects , Clone Cells/drug effects , Clone Cells/metabolism , Clone Cells/pathology , Friend murine leukemia virus , Isoniazid/pharmacology , Leukemia, Erythroblastic, Acute/pathology , Leukemia, Experimental/pathology , MiceABSTRACT
Multiple hemoglobins are synthesized in the T3C12 murine erythroleukemic cells after induction with butyric acid and hemin. The pI values determined by IEF and globin composition of these hemoglobins suggests that A, E2, and E3 hemoglobins are synthesized in T3C12 cells. The embryonic mouse globins x, y, z and alpha from the yolk sac cells could be separated by acid urea triton x-100 electrophoresis. The mobility of the globins are the following alpha greater than z greater than y greater than x.
Subject(s)
Hemoglobins/biosynthesis , Leukemia, Erythroblastic, Acute/blood , Animals , Cells, Cultured , Globins/analysis , Hemoglobins/analysis , Mice , Mice, Inbred C57BLABSTRACT
The addition of 5-azacytidine to erythroleukemic cells which were induced to differentiate with DMSO or BA altered the expression of the hemoglobins. After the addition of 5-azacytidine there was an increase in hemoglobin synthesis especially in the embryonic E2 band. The beta-globin increased in synthesis after 5-azacytidine treatment. The level of hemoglobin synthesis in DMSO-induced cells is less than BA-induced cells while the effect of the 5-azacytidine stimulation was greater with DMSO induction than with BA induction.
Subject(s)
Azacitidine/pharmacology , Hemoglobins/biosynthesis , Leukemia, Erythroblastic, Acute/metabolism , Animals , Butyrates/pharmacology , Butyric Acid , Cell Differentiation , Dimethyl Sulfoxide/pharmacology , Fetal Hemoglobin/biosynthesis , Friend murine leukemia virus , Gene Expression Regulation , Isoelectric Focusing , Mice , Protein BiosynthesisABSTRACT
HL-60 promyelocytic leukemic cells can be induced to differentiate into granulocytes or macrophages. Reduced glutathione lyses undifferentiated HL-60 cells but has minimal effect on their differentiated counterparts. The addition of reduced glutathione to HL-60 promyelocytic leukemic cells retards cell growth and lyses cells. HL-60 cells can be induced to differentiate into granulocytes with dimethylsulfoxide butyric acid or into macrophages with 12-O-tetradecanoylphorbol-13-acetate. After treatment of HL-60 cells with these inducing agents the HL-60 cells become unresponsive to the effects of glutathione.
