ABSTRACT
Patients with spinal cord injury (SCI) have an increased risk of developing esophageal, bladder and hematologic malignancies compared with the normal population. In the present study, we aimed to identify, through in silico analysis, miRNAs and their target genes related to the three most frequent types of cancer in individuals with SCI. In a previous study, we reported a pattern of expression of miRNAs in 17 sedentary SCI males compared with 22 healthy able-bodied males by TaqMan OpenArray. This list of miRNAs deregulated in SCI patients was uploaded to miRWALK2.0 to predict the target genes and pathways of selected miRNAs. We used Cytoscape software to construct the network displaying the miRNAs and their gene targets. Among the down-regulated miRNAs in SCI, 21, 19 and 20 miRNAs were potentially associated with hematological, bladder and esophageal cancer, respectively, and three target genes (TP53, CCND1 and KRAS) were common to all three types of cancer. The three up-regulated miRNAs were potentially targeted by 18, 15 and 10 genes associated with all three types of cancer. Our current bioinformatics analysis suggests the potential influence of several miRNAs on the development of cancer in SCI. In general, these data may provide novel information regarding potential molecular mechanisms involved in the development of cancer among individuals with SCI. Further studies aiming at understanding how miRNAs contribute to the development of the major cancers that affect patients after SCI may help elucidate the role of these molecules in the pathophysiology of the disease.
Subject(s)
Cell-Free Nucleic Acids/blood , Computational Biology , MicroRNAs/blood , Spinal Cord Injuries/blood , Adult , Cell-Free Nucleic Acids/classification , Esophageal Neoplasms/blood , Esophageal Neoplasms/genetics , Gene Expression Regulation, Neoplastic/genetics , Hematologic Neoplasms/blood , Hematologic Neoplasms/genetics , Humans , Male , MicroRNAs/classification , Sedentary Behavior , Spinal Cord Injuries/pathology , Urinary Bladder Neoplasms/blood , Urinary Bladder Neoplasms/geneticsABSTRACT
OBJECTIVE: To investigate the longitudinal effects of wheelchair rugby (WR) training on body composition of subjects with tetraplegia. DESIGN: Subjects were evaluated at baseline and after WR training. SETTING: Faculty of physical education settings. PARTICIPANTS: Individuals with tetraplegia (N=13; age, 26.6±6.0y). INTERVENTIONS: Four sessions per week of WR training composed by aerobic and anaerobic activities and technical and tactical aspects of WR. The average time of intervention was 8.1±2.5 months. MAIN OUTCOME MEASURES: Body composition assessed by dual-energy x-ray absorptiometry. RESULTS: After training, fat mass was significantly reduced in the whole body (15,191±4603 vs 13,212±3318 g, P=.016), trunk (7058±2639 vs 5693±1498 g, P=.012), and legs (2847±817 vs 2534±742 g, P=.003). Conversely, increased bone mineral content (183±35 vs 195±32 g, P=.01) and fat-free mass (2991±549 vs 3332±602 g, P=.016) in the arms and reduced bone mineral content in the trunk (553±82 vs 521±86 g, P=.034) were observed after training. Furthermore, no significant correlation between the duration of training and changes in body composition was detected. CONCLUSIONS: Regular WR training increased lean mass and bone mineral content in the arms and decreased total body fat mass. Conversely, WR training was associated with decreased bone mineral content in the trunk. These results suggest that regular WR training improves body composition in subjects with tetraplegia.
Subject(s)
Body Composition , Football/physiology , Physical Conditioning, Human/physiology , Quadriplegia/physiopathology , Sports for Persons with Disabilities/physiology , Wheelchairs , Absorptiometry, Photon , Adiposity , Adult , Arm , Bone Density , Exercise/physiology , Humans , Leg , Longitudinal Studies , Male , Pilot Projects , Torso , Young AdultABSTRACT
Avaliou-se histomorfológicamente, polpas de premolares humanos, eleitos para extraçäo por motivos ortodônticos, e que tiveram braquetes cerâmicos descolados com laser CO² e irradiados por 2 segundos, com potências de 10, 14 e 18 watts in vivo. Verificou-se as diferentes reaçöes das polpas desses dentes levando-se em conta o intervalo de 7, 14 e 21 dias decorridos entre a irradiaçäo e o período de extraçäo. Observou-se que a polpa näo sofreu reaçöes inflamatórias ou degenerativas irreversíveis, mostrando no entanto, nítidas evidências de defesa com reparaçäo na histofisiologia tecidual a partir de 14 dias após a irradiaçäo com laser CO² sobre os braquetes cerâmicos
Subject(s)
Humans , Male , Female , Dental Pulp , Orthodontic Brackets , Tooth Extraction , OrthodonticsABSTRACT
The present study was done to estimate the prevalence of Hepatitis A (HAV), B (HBV), C (HCV), and E (HEV) infection in the general population residing in the municipality of Säo Paulo, and to evaluate the level of knowledge related to the various modes of infection transmission by and protection against the different viruses. Blood samples and health questionnaires were collected from 1,059 individuals. The study design used an inductive metod of predictive statistical inferences through randomized sampling stratifield by sex, age and residence region. The estimated prevalence rated found were: Hepatitis A = 66.59 percent (63.75 percent - 69.44 per cent CI); Hepatitis B = 5.94 percent (4.50 percent-7.35 percent); Hepatitis C =1.42 percent (0,70 percent - 2.12 percent); Hepatitis E = 1.68 percent (0.91 percent - 2.46 percent). The frequency of hepatitis was similar in males and females. HAV showed an estimated prevalence of 56.16 percent in the population up to 17 years old, increasing to 65.30 percent in individuals between 18 and 29 years. The infection reached its peak of 90 percent in individuals 40 years of age or older. The study showed a greater tendency of dissemination of HBV among the population between 15 and 17 years. This specific age group showed an estimated prevalence of active infection of 1.04 percent (0.43 percent - 1.65 percent CI), and also demonstrated an ascending level of acquired immunity with an estimated prevalence of 4.90 percent (3.60 percent - 6.20 percent CI). HCV demonstrated an estimated prevalence of 1.42 percent (0.70 percent - 2.12 percent CI). This specific infection occurred more frequently among adults 30 years of age or older, with the prevalence reaching a peak of 3.80 percent among the group aged 50 to 59 years. HEV showed zero prevalence among the age group between 2 and 9 years. This was followed by a slighty ascending rate starting from age 10, with an estimated prevalence of 1.05 percent (0.94 percent - 3.04 percent CI) among those 10 to 14 years of age....
Subject(s)
Humans , Male , Female , Child, Preschool , Child , Adolescent , Adult , Middle Aged , Hepatitis A , Hepatitis B , Hepatitis C , Hepatitis E , Prevalence , Brazil , Data Interpretation, Statistical , Health Promotion , Hepatitis , Population Surveillance , Seroepidemiologic StudiesABSTRACT
We determined the effect of 13 days of treatment with 2.0 mg/kg haloperidol, 4.0 mg/kg bromocriptine or 2.0 mg/kg amphetamine on the number of tumor cells of mice bearing Ehrlich ascites carcinoma. The dopaminergic blocker significantly reduced the number of tumor cells of experimental mice, but the two dopamine-mimetic drugs used did not significantly affect tumor development. These results suggest that although neuroleptic drugs to inhibit Ehrlich ascites tumors, this effect does not seem to be related to changes in dopaminergic neuronal transmission.
Subject(s)
Amphetamine/therapeutic use , Bromocriptine/therapeutic use , Carcinoma, Ehrlich Tumor/drug therapy , Haloperidol/therapeutic use , Analysis of Variance , Animals , Carcinoma, Ehrlich Tumor/pathology , Disease Models, Animal , Male , Mice , Neoplasm Transplantation , Random AllocationABSTRACT
Apresentamos 5 casos de tuberculose faringo-laríngea e resaltamos que apesar dessa patologia ser secundária à tuberculose pulmonar, a hipótese diagnóstica de tuberculose foi levantada após os exames otorrinolaringológicos. Destacamos ainda, a importância das queixas otorrinolaringológicas permitindo o diagnóstico de tuberculose pulmonar
Subject(s)
Humans , Male , Female , Adult , Middle Aged , Tuberculosis, Laryngeal/diagnosis , Pharyngeal Diseases/diagnosis , Pharyngeal Diseases/etiology , Tuberculosis, Laryngeal/etiology , Tuberculosis, Pulmonary , Tuberculosis, Pulmonary/complicationsABSTRACT
É apresentado um caso de rinoliquorréia, que surgiu 12 anos após traumatismo craniano. Tendo o paciente apresentado nos últimos anos, duas meningites. A perda líquida nasal, cessou apenas após a correçäo cirúrgica de três lesöes encontradas: a nível das células da mastóide, a nível da lâmina crivosa do etmóide e por correçäo de meningoencefalocele frontal. Trata-se de um caso peculiar, para o qual näo encontramos semelhante na literatura consultada
Subject(s)
Humans , Female , Middle Aged , Cerebrospinal Fluid Rhinorrhea/diagnosis , Cerebrospinal Fluid Rhinorrhea/etiology , Cerebrospinal Fluid Rhinorrhea/surgery , Skull , Encephalocele/complications , Encephalocele/surgery , Meningocele/complications , Meningocele/surgery , Paranasal Sinuses , Postoperative Complications , Recurrence , Tomography, X-Ray ComputedABSTRACT
É estudado um caso de tumor vascular benigno do antro maxilar, o hemangioma. Säo revistos aspectos da etiopatogenia do tumor e da formaçäo de flebólitos em seu interior. É feita revisäo da literatura que demonstra ser incomum o achado desse angioma. Poucos casos estäo descritos na literatura com a demonstraçäo de formaçöes cálcicas em seu interior. A conduta terapêutica tomada e o resultado da mesma, säo comentados
Subject(s)
Humans , Female , Middle Aged , Hemangioma/diagnosis , Maxillary Neoplasms/diagnosis , Maxillary Sinus/pathology , Hemangioma/pathology , Hemangioma/surgery , Maxillary Neoplasms/pathology , Maxillary Neoplasms/surgery , Tomography, X-Ray ComputedABSTRACT
We determined the effect of 13 days of treatment with 2.0 mg/kg haloperidol, 30.0 mg/kg metoclopramide or 4.0 mg/kg domperidone on the number of tumor cells of mice bearing Ehrlich ascites carcinoma. The three dopaminergic blockers significantly reduced the number of tumor cells of experimental mice. The mean +/- SEM number of tumor cells x 10(6)/ml saline lavage was 25.5 +/- 5.9 for the haloperidol group, 36.8 +/- 4.7 for the metoclopramide group, 25.3 +/- 3.5 for the domperidone group and 54.0 +/- 9.0 for the control mice (treated with 0.9% NaCl). In a second experiment, treatment with 0.5 and 2.0 mg/kg haloperidol showed that the antitumor effect of this drug was dose dependent. The possible mechanisms underlying these results (such as an increase in prolactin levels or a direct action of these drugs on lymphocytes) are discussed in light of the specific pharmacological properties of each dopaminergic blocker.
Subject(s)
Antineoplastic Combined Chemotherapy Protocols/pharmacology , Carcinoma, Ehrlich Tumor/drug therapy , Analysis of Variance , Animals , Domperidone/administration & dosage , Dose-Response Relationship, Drug , Drug Screening Assays, Antitumor , Haloperidol/administration & dosage , Metoclopramide/administration & dosage , MiceABSTRACT
We determined the effect of 13 days of treatment with 2.0 mg/kg haloperidol, 30.0 mg/kg metoclopramide or 4.0 mg/kg domperidone on the number of tumor cells of mice bearing Ehrlich ascites carcinoma. The three dopaminergic blockers significantly reduced the number of tumor cells of experimental mice. The mean ñ SEM number of tumor cvells x 10******6/ml saline lavage was 25.5 ñ 5.9 for the haloperidol group, 36.8 ñ 4.7 for the metoclopramide group, 25.3 ñ 3.5 for the domperidone group and 54.0 ñ 9.0 for the control mice (treated with 0.9% NaCl). In a second experiment, treatment with 0.5 and 2.0 mg/kg haloperidol showed that the antitumor effect of this drug was dose dependent. The possible mechanisms underlying these results (such as an increase in prolactin levels or a direct action of these drugs on lymphocytes) are discussed in light of the specific pharmacological properties of each dopaminergic blocker
Subject(s)
Animals , Mice , Antineoplastic Combined Chemotherapy Protocols/pharmacology , Carcinoma, Ehrlich Tumor/drug therapy , Analysis of Variance , Domperidone/administration & dosage , Dose-Response Relationship, Drug , Drug Screening Assays, Antitumor , Haloperidol/administration & dosageABSTRACT
Os autores reportam um caso de tuberculose nasal sem evidência de comprometimento pulmonar. A hipótese diagnóstica inicial foi reforçada quando a biópsia de um nódulo na mama esquerda revelou Tuberculose. O caso relatado mostra a necessidade de tratamento antituberculoso por tempo prolongado para desaparecimento dos sintomas nasais
Subject(s)
Humans , Adult , Female , Nose Diseases/diagnosis , Tuberculosis/diagnosis , Breast/pathology , Nose Diseases/pathology , Tuberculosis/pathologyABSTRACT
O caso apresentado refere-se ao paciente A.A.M.S., 6 anos de idade, branco, sexo masculino, portador de cisto dentígero bilateral de mandíbula, associado aos primeiros molares permanentes inferiores näo erupcionados. O tratamento realizado cirúrgicamente constou de enucleaçäo dos cistos dentígeros complementado por avulsäo dos primeiros molares inferiores permanentes. Após 12 meses, controle radiográfico mostrou reparaçäo óssea normal
Subject(s)
Child , Humans , Male , Mandibular Neoplasms/surgery , Molar/surgery , Dentigerous Cyst/surgery , Follow-Up StudiesABSTRACT
Twenty-three idiopathic oligoasthenospermic patients were subdivided into three classes according to degree of sperm concentration and typical sperm motility percentage. These patients were treated with 75 I.U. of Urofollitropin every other day for three months. The patients with moderate oligoasthenospermia responded more favorably than patients with severe or more severe oligoasthenospermia.