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1.
Genet Mol Res ; 15(3)2016 Sep 02.
Article in English | MEDLINE | ID: mdl-27706690

ABSTRACT

The current study aims to evaluate the macroscopic and histological effects of autologous mesenchymal stem cells (MSC) and platelet-rich plasma on knee articular cartilage regeneration in an experimental model of osteoarthritis. Twenty-four rabbits were randomly divided into four groups: control group, platelet-rich plasma group, autologous MSC undifferentiated group, and autologous MSC differentiated into chondrocyte group. Collagenase solution was used to induce osteoarthritis, and treatments were applied to each group at 6 weeks following osteoarthritis induction. After 60 days of therapy, the animals were euthanized and the articular surfaces were subjected to macroscopic and histological evaluations. The adipogenic, chondrogenic, and osteogenic differentiation potentials of MSCs were evaluated. Macroscopic and histological examinations revealed improved tissue repair in the MSC-treated groups. However, no difference was found between MSC-differentiated and undifferentiated chondrocytes. We found that MSCs derived from adipose tissue and platelet-rich plasma were associated with beneficial effects in articular cartilage regeneration during experimental osteoarthritis.


Subject(s)
Chondrogenesis , Mesenchymal Stem Cell Transplantation , Osteoarthritis/therapy , Platelet Transfusion , Platelet-Rich Plasma/cytology , Regeneration/physiology , Adipocytes/cytology , Adipocytes/physiology , Animals , Cartilage, Articular/pathology , Cell Differentiation , Chondrocytes/cytology , Chondrocytes/physiology , Collagenases , Disease Models, Animal , Humans , Injections, Intra-Articular , Knee Joint/pathology , Mesenchymal Stem Cells/cytology , Mesenchymal Stem Cells/physiology , Osteoarthritis/chemically induced , Osteoarthritis/pathology , Osteoblasts/cytology , Osteoblasts/physiology , Rabbits , Transplantation, Autologous
2.
Genet Mol Res ; 14(1): 339-48, 2015 Jan 23.
Article in English | MEDLINE | ID: mdl-25729966

ABSTRACT

The use of mesenchymal stem cells (MSCs) in experimental, clinical, and therapeutic trials has grown in recent years. However, the issue remains of whether these procedures are completely safe for transplant patients. Therefore, this study was designed and carried out with the aim of evaluating two different comet assay protocols for genomic damage pattern analysis in MSCs derived from adipose tissue. The analyzed and interpreted results suggest that genetic testing is needed to support clonal expansion safety in cell therapy procedures with MSCs. Furthermore, they also suggest that if the comet assay technique would be used as a genomic integrity screening assay, the protocol performed at pH = 12 (that yielded a frequency of damaged cells: tail intensity = 9.50 ± 0.60, tail moment = 0.0122 ± 0.0007; results are reported as means ± standard deviation) would be indicated as genomic damage, and that subsequent single-strand breaks occur at pH > 13 (frequency of damaged cells: tail intensity = 30.71 ± 4.23, tail moment = 0.0447 ± 0.0073). Our study demonstrates that, in the era of regenerative medicine, it is necessary to standardize and establish a battery of tests in order to identify genomic damage prior to MSC transplantation.


Subject(s)
Adipose Tissue/cytology , Comet Assay/methods , Genome , Mesenchymal Stem Cells/cytology , Adipogenesis/drug effects , Animals , Cell Count , Cell Shape/drug effects , Cell Survival/drug effects , Chondrogenesis/drug effects , DNA Damage , Hydrogen-Ion Concentration , Mesenchymal Stem Cells/drug effects , Mutagens/toxicity , Osteogenesis/drug effects , Rabbits
3.
Transplant Proc ; 46(5): 1591-3, 2014 Jun.
Article in English | MEDLINE | ID: mdl-24834857

ABSTRACT

BACKGROUND: Melatonin is a free radical scavenger with important actions in the study of renal ischemia and reperfusion (I/R). This study evaluated possible renal protection of high doses of melatonin in an experimental model of I/R in which rats were submitted to acute hyperglycemia under anesthesia with isoflurane. METHOD: Forty-four male Wistar rats, weighing more than 300 g, were randomly divided into 5 groups: G1, sham (n = 10); G2, melatonin (n = 10; 50 mg.kg(-1)); G3, hyperglycemia (n = 9; glucose 2.5 g.kg(-1)); G4, hyperglycemia/melatonin (n = 10; 2.5 g.kg(-1) glucose + melatonin 50 mg.kg(-1)); and G5, I/R (n = 5). In all groups, anesthesia was induced with 4% isoflurane and maintained with 1.5% to 2.0% isoflurane. Intraperitoneal injection of melatonin (G1, G4), glucose (G3, G4), or saline (G1, G5) was performed 40 minutes before left renal ischemia. Serum plasma values for creatinine and glucose were determined at baseline (M1), immediately following reperfusion (M2), and 24 hours after completion of the experiment (M3). Histological analysis was performed to evaluate tubular necrosis (0-5). RESULTS: Serum glucose was higher at M2 in the groups supplemented with glucose, hyperglycemia (356.00 ± 107.83), and hyperglycemia/melatonin (445.3 ± 148.32). Creatinine values were higher at T3 (P = .0001) for I/R (3.6 ± 0.37), hyperglycemia/melatonin (3.9 ± 0.46), and hyperglycemia (3.71 ± 0.69) and lower in the sham (0.79 ± 0.16) and melatonin (2.01 ± 1.01) groups, P < .05. Histology showed no necrosis injury in the G1, lesion grade 2 in the G2, and severe acute tubular necrosis in the G3: (grade 4), G4: (grade 5) and G5: (grade 4) groups (P < .0001). DISCUSSION: Melatonin protected the kidneys submitted to I/R in rats without hyperglycemia; however, this did not occur when the I/R lesion was associated with hyperglycemia. CONCLUSIONS: Due to its antioxidant and antiapoptotic action, melatonin was able to mitigate, but not prevent acute tubular necrosis in rats with hyperglycemia under anesthesia by isoflurane.


Subject(s)
Hyperglycemia/complications , Kidney/drug effects , Melatonin/pharmacology , Reperfusion Injury/prevention & control , Animals , Dose-Response Relationship, Drug , Kidney/blood supply , Kidney/physiopathology , Male , Rats , Rats, Wistar , Reperfusion Injury/complications
4.
Arq. bras. med. vet. zootec ; 65(4): 939-945, Aug. 2013. ilus, graf, tab
Article in English | LILACS | ID: lil-684445

ABSTRACT

Stem cell therapy has shown promising results in tendinitis and osteoarthritis in equine medicine. The purpose of this work was to characterize the adipose-derived mesenchymal stem cells (AdMSCs) in horses through (1) the assessment of the capacity of progenitor cells to perform adipogenic, osteogenic and chondrogenic differentiation; and (2) flow cytometry analysis using the stemness related markers: CD44, CD90, CD105 and MHC Class II. Five mixed-breed horses, aged 2-4 years-old were used to collect adipose tissue from the base of the tail. After isolation and culture of AdMSCs, immunophenotypic characterization was performed through flow cytometry. There was a high expression of CD44, CD90 and CD105, and no expression of MHC Class II markers. The tri-lineage differentiation was confirmed by specific staining: adipogenic (Oil Red O), osteogenic (Alizarin Red), and chondrogenic (Alcian Blue). The equine AdMSCs are a promising type of adult progenitor cell for tissue engineering in veterinary medicine.


O uso de células tronco tem demonstrado resultados promissores na terapia da tendinite e osteoartrite na medicina equina. O objetivo deste trabalho foi caracterizar as células tronco mesenquimais derivadas do tecido adiposo (AdCTMs) em cavalos através da (1) avaliação da capacidade das células progenitoras para realizar a diferenciação adipogênica, osteogênica e condrogênica; e (2) através da análise por citometria de fluxo, utilizando os marcadores stemness relacionados: CD44, CD90, CD105 e MHC de Classe II. Cinco cavalos sem raça definida, de 2 a 4 anos de idade foram utilizados para a coleta do tecido adiposo da base da cauda. Após o isolamento e cultivo das AdCTMs, a caracterização imunofenotípica foi realizada pela citometria de fluxo. Houve alta expressão dos marcadores CD44, CD90 e CD105, e não houve expressão do MHC Classe II. A diferenciação foi confirmada pela coloração específica: adipogênica (Oil Red O), osteogênico (Alizarin Red), e condrogênico (Alcian Blue). As AdCTMs são um tipo promissor de células progenitoras adulta para a engenharia tecidual na medicina veterinária.


Subject(s)
Animals , Stem Cells/cytology , Immunophenotyping , Adipose Tissue/physiology , Horses/classification
5.
J Wound Care ; 22(4): 186-8, 190, 192-3, 2013 Apr.
Article in English | MEDLINE | ID: mdl-23702671

ABSTRACT

Venous leg ulcers (VLUs) represent the most advanced stage of chronic venous insufficiency. Despite the large body of knowledge available regarding the risk factors and aetiopathogeny of the condition, patients referred to public health care systems in developing countries often do not receive adequate diagnosis or early treatment, leading to clinical evolution and disease recurrence. This review collates updated information about the epidemiology, risk factors, aetiopathogeny, diagnosis, ulcer healing methods and determinant factors of the pernicious cycle of VLUs in developing countries, with a focus on the Brazilian setting.


Subject(s)
Skin Care/methods , Varicose Ulcer/therapy , Brazil/epidemiology , Humans , Recurrence , Risk Factors , Varicose Ulcer/diagnosis , Varicose Ulcer/epidemiology , Varicose Ulcer/etiology , Wound Healing/physiology
6.
Transplant Proc ; 43(10): 3618-21, 2011 Dec.
Article in English | MEDLINE | ID: mdl-22172815

ABSTRACT

BACKGROUND: Hyperglycemia is associated with a decreased tolerance to ischemia and an increased severity of renal ischemia reperfusion (I/R) injury. It has been suggested that erythropoietin (EPO) attenuates this effect in normoglycemic animals. This study sought to examine the effects of EPO on treatment renal I/R injury (IRI) in transiently hyperglycemic rats. MATERIAL AND METHODS: Twenty-eight male Wister rats anesthetized with isoflurane received glucose (2.5 g.kg(-1) intraperitoneally) before right nephrectomy. They were randomly assigned to four groups: sham operation (S); IRI (ISO); IRI+EPO, (600 UI kg(-1) low-dose EPO [EL]); and IRI+EPO 5000 UI kg(-1) (high-dose EPO [EH]). IRI was induced by a 25-minute period of left renal ischemia followed by reperfusion for 24 hours. Serum creatinine and glucose levels were measure at baseline (M1), immediately after the ischemic period (M2), and at 24 hours after reperfusion (M3). After sacrificing the animals, left kidney specimens were submitted for histological analysis including flow cytometry to estimate tubular necrosis and the percentages of apoptotic, dead or intact cells. RESULTS: Scr in the ISO group was significantly higher at M3 than among the other groups. Percentages of early apoptotic cells in ISO group were significantly higher than the other groups. Percentages of late apoptotic cells in S and ISO groups were significantly greater than EL and EH groups. However, no significant intergroup differences were observed regarding the incidence of tubular necrosis. CONCLUSIONS: Our results suggested that, although not preventing the occurrence of tubular necrosis, EPO attenuated apoptosis and glomerular functional impairment among transiently hyperglycemic rats undergoing an ischemia/reperfusion insult.


Subject(s)
Apoptosis/drug effects , Erythropoietin/pharmacology , Hyperglycemia/complications , Kidney/drug effects , Kidney/surgery , Nephrectomy/adverse effects , Reperfusion Injury/prevention & control , Animals , Blood Glucose/metabolism , Creatinine/blood , Cytoprotection , Disease Models, Animal , Epoetin Alfa , Flow Cytometry , Kidney/blood supply , Kidney/pathology , Kidney Glomerulus/drug effects , Kidney Glomerulus/pathology , Kidney Tubules/drug effects , Kidney Tubules/pathology , Male , Necrosis , Rats , Rats, Wistar , Recombinant Proteins/pharmacology , Reperfusion Injury/etiology , Reperfusion Injury/pathology , Time Factors
7.
Transplant Proc ; 41(10): 4080-2, 2009 Dec.
Article in English | MEDLINE | ID: mdl-20005344

ABSTRACT

BACKGROUND: The purpose of this investigation was to examine the effect of isoflurane, remifentanil, and preconditioning in renal ischemia/reperfusion injury (IRI). METHODS: All 52 male Wistar rats were anesthetized with isoflurane, intubated and mechanically ventilated. The animals were randomly divided into: S group (sham; n = 11) that underwent only right nephrectomy; as well as the I group of right nephrectomy and ischemia for 45 minutes by clamping of left renal artery. (n = 11); the IP (n = 9), the R (n = 10), and the RP (n = 11) groups. In addition, the R and RP animals received remifentanil (2 microg.kg(-1).min(-1)) during the entire experiment. The IP and RP group underwent ischemic preconditioning (IPC = three cycles of 5 minutes). Serum creatinine values were determined before and after IRI, as well as 24 hours later. In addition to an Histological study, cells from the left kidney were evaluated for apoptosis by flow cytometry (FCM). RESULTS: The Creatinine value of 0.8 +/- 0.2 mg/dl in the S group was significantly lower at 24 hours than the I 3.9 +/- 1.5 mg/dl; IP 2.6 +/- 1.7 mg/dl; R 3.3 +/- 2.8 mg/dl; or RP 1.8 +/- 0.5 mg/dl groups. The RP group value was significantly lower than those of the I, IP, and R groups (p < 0.05). The S group showed less proximal tubular cell damage than the I, IP, R, and RP groups (p < 0.05). The percentages of apoptotic cells (FITC(+)/PI(-)) were: S group = 11.6 +/- 6.5; I = 16.7 +/- 7.3; IP = 37.0 +/- 28.4; R = 11.7 +/- 6.6, and RP = 8.8 +/- 1.5. The difference between the IP vs RP group was significant. Similar percentages of necrotic cells (FITC(+)/PI(+)) and intact cells (FITC(-)/PI(-)) were observed among the groups. CONCLUSIONS: Ischemic preconditioning showed no protective effect in the isoflurane group (IP) but when isoflurane was administered associated with remifentanil (RP), there was a beneficial effect on the kidney, as demonstrated by flow cytometry and serum creatinine values.


Subject(s)
Ischemic Preconditioning/adverse effects , Isoflurane/therapeutic use , Kidney/pathology , Piperidines/therapeutic use , Reperfusion Injury/prevention & control , Anesthetics, Inhalation/pharmacology , Anesthetics, Inhalation/therapeutic use , Anesthetics, Intravenous/pharmacology , Anesthetics, Intravenous/therapeutic use , Animals , Apoptosis/drug effects , Creatinine/blood , Kidney/drug effects , Male , Rats , Rats, Wistar , Remifentanil , Renal Artery , Reperfusion Injury/pathology
8.
Int J Immunogenet ; 32(3): 165-6, 2005 Jun.
Article in English | MEDLINE | ID: mdl-15932620

ABSTRACT

The human platelet alloantigen (HPA) 4b allele is rarely observed in Caucasians and the observed incidence in Asians is usually lower than 1.0%. We report the first Brazilian with the allele HPA-4b, and were able to determined that he inherited it from his father.


Subject(s)
Alleles , Antigens, Human Platelet/blood , Antigens, Human Platelet/genetics , Blood Platelets/immunology , Adult , Blood Platelets/metabolism , Brazil , Histocompatibility Testing , Humans , Male
9.
Rev Fr Transfus Hemobiol ; 35(4): 299-309, 1992 Jul.
Article in French | MEDLINE | ID: mdl-1476585

ABSTRACT

The use of red blood cells coated with complement fractions using low ionic strength saline conditions is part of the evaluation of anti-complement antibodies, specially monoclonal antibodies used for antiglobulin reagents. This work shows that such cells stored in liquid nitrogen allow satisfactory long-term studies except for the anti-C3g antibodies.


Subject(s)
Blood Preservation , Complement System Proteins/immunology , Coombs Test/standards , Cryopreservation , Erythrocytes/immunology , Anemia, Hemolytic, Autoimmune/blood , Anemia, Hemolytic, Autoimmune/immunology , Antibodies, Monoclonal/immunology , Antibody Specificity , Blood Preservation/methods , Complement C3/immunology , Cryopreservation/methods , False Positive Reactions , Hemagglutination Tests/standards , Humans , Hypotonic Solutions , Nitrogen , Reference Standards , Sensitivity and Specificity , Sodium Chloride
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