Oropharyngeal carriage and penicillin resistance of Neisseria meningitidis in primary school children in Manisa, Turkey.

INTRODUCTION: To determine the oropharyngeal carriage rates and serogroups of Neisseria meningitidis in primary school children in Manisa, Turkey as well as the prevalence and penicillin resistance of N. meningitidis. MATERIALS AND METHODS: Throat swabs obtained from 1128 children were cultured and recovered organisms were tested by disk diffusion method and the E-test for antimicrobial susceptibilities. RESULTS: The carriage rate of N. meningitidis in our region was 6.2% (71 strains) and the serogroups identified were serogroups A (28.1%), B (22.5%), C (35.2%), D (2.8%) and W-135 (11.2%). Penicillin resistance was found in 16 strains (22.5%), while beta-lactamase activity was found in none. CONCLUSIONS: The carriage rate of N. meningitidis and serogroups are similar to the rates reported in other countries. Continued surveillance of meningococci for antimicrobial resistance will allow early detection of changes in susceptibility patterns that might affect recommendations for chemoprophylaxis as well as for treatment.

Incidence of Trichomonas vaginalis among women having vaginal discharge, in Manisa, Turkey.

Trichomoniasis characterise with a foamy yellowish odorous discharge, is an infection that causes superficial defects and necrotic ulcers in mucosa, and is spreadable with sexual contact. In T. vaginalis infection, the diagnosis can be made after the examination of saline wet mount preparations, stained smears preparation and cultivation of the vaginal discharge, the urine and the materials obtained from prostate secretion in males. In the present study, vaginal discharge samples obtained from 207 patients applied to Celal Bayar University, Medical Faculty Research Hospital, Manisa with vaginal discharge complaints were examined by utilising wet mount preparations, Giemsa staining method and CPLM cultivation method. In 27 of them (13.1%) T. vaginalis was detected by both saline wet mount preparation and Giemsa staining method. But as a result of the cultivation in CPLM media of the vaginal discharge materials collected from infected samples reproduction was observed in only 21 of them (10.2%). A treatment of Secnidazole by 2 gr. unique dose was applied to infected patients and their spouses and a complete recovery was followed up both microscopically and clinically.

PIP: The incidence of Trichomonas vaginalis was investigated in 207 women 18-45 years of age who presented to Celal Bayar University Hospital in Manisa, Turkey, with vaginal discharge. In 27 women (13.1%), T. vaginalis was detected by both direct microscopy of saline wet mount preparations and the microscopic examination of Giemsa stained smears. After cultivation of the vaginal discharge samples in CPLM media, T. vaginalis reproduction was seen in 21 samples (10.2%). All women with T. vaginalis as well as their spouses were treated with 2 g of Secnidazole and complete recovery was observed at follow-up 1 week after treatment ended.