Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 8 de 8
Filter
Add more filters










Database
Language
Publication year range
2.
Article in English | MEDLINE | ID: mdl-15325340

ABSTRACT

The intestinal fatty acid-binding protein (I-FABP) shows binding specificity for long-chain fatty acids and is proposed to be involved in uptake of dietary fatty acids and their intracellular transport. We have determined the sequence of the gene encoding I-FABP in zebrafish. The zebrafish I-FABP gene contains four exons interrupted by three introns. Radiation hybrid mapping assigned the I-FABP gene to linkage group 1. A 924 bp sequence 5' upstream of the initiation codon in the I-FABP gene contained several putative cis-acting regulatory elements. In adult zebrafish, reverse transcription-polymerase chain reaction (RT-PCR) detected I-FABP mRNA in intestine, brain, liver, muscle and testis. Quantitative RT-PCR demonstrated that I-FABP mRNA was most abundant in intestine, followed by brain. I-FABP mRNA levels were very low in muscle, testis, heart, liver, skin and ovary. RT-PCR using total RNA extracted from zebrafish embryos detected I-FABP mRNA as early as 12 h post-fertilization. Whole-mount in situ hybridization to zebrafish embryos detected I-FABP mRNA in the yolk syncytial layer (YSL) at early somitogenesis. Later during embryonic development the I-FABP mRNA was detected in the intestinal bulb, liver and pancreas primordium. Expression in YSL, liver or pancreas has not been previously reported for fish or mammalian I-FABP genes and may be related to specific physiological differences between fishes and mammals.


Subject(s)
Carrier Proteins/biosynthesis , Carrier Proteins/genetics , Genetic Linkage , Zebrafish Proteins/biosynthesis , Zebrafish Proteins/genetics , Amino Acid Sequence , Animals , Base Sequence , Cloning, Molecular , Exons , Fatty Acid-Binding Proteins , Fatty Acids/chemistry , Introns , Molecular Sequence Data , Promoter Regions, Genetic , RNA/chemistry , RNA, Messenger/metabolism , Radiation Hybrid Mapping , Reverse Transcriptase Polymerase Chain Reaction , Sequence Analysis, DNA , Sequence Homology, Amino Acid , Time Factors , Tissue Distribution , Transcription, Genetic , Zebrafish
3.
Gene Expr Patterns ; 4(4): 379-87, 2004 Jul.
Article in English | MEDLINE | ID: mdl-15183304

ABSTRACT

A gene for the zebrafish brain-type fatty acid-binding protein (fabp7b) was identified and its structure defined. The zebrafish fabp7b gene spans 1479 bp and consists of four exons encoding 24, 58, 34 and 16 amino acids, respectively, which is identical to the structure of the fabp7a gene previously described. The complete fabp7b cDNA was isolated by 5' and 3' RACE and its nucleotide sequence determined. The deduced amino acid sequence of FABP7B encoded by the zebrafish fabp7b gene shares 82% identity with that of FABP7A encoded by the zebrafish fabp7a gene. A single transcription start site for the fabp7b gene was mapped by 5' RNA ligase-mediated RACE. Phylogenetic analysis indicated that the duplication of the fabp7 genes occurred in the fish lineage after their divergence from mammals. The zebrafish fabp7b gene was assigned to linkage group 20 by radiation hybrid mapping. Reverse transcription-polymerase chain reaction detected fabp7b transcripts in the same adult tissues as fabp7a transcripts. In the brain, levels of fabp7b transcripts were lower than fabp7a transcripts. Whole-mount in situ hybridization showed that the zebrafish fabp7a transcripts were distributed in the early developing central nervous system. In addition to being expressed in the developing brain and retina, zebrafish fabp7b mRNA was also detected in the swim bladder and pharynx during the embryonic to larval transitory phase.


Subject(s)
Carrier Proteins/biosynthesis , Central Nervous System/embryology , Gene Expression Regulation, Developmental/physiology , Genes, Duplicate/physiology , Nerve Tissue Proteins/biosynthesis , Zebrafish Proteins/biosynthesis , Zebrafish/embryology , Animals , Base Sequence , Carrier Proteins/genetics , Central Nervous System/metabolism , Chromosome Mapping , Fatty Acid-Binding Protein 7 , Fatty Acid-Binding Proteins , Gene Expression Regulation, Developmental/genetics , Genes, Duplicate/genetics , Molecular Sequence Data , Nerve Tissue Proteins/genetics , Organ Specificity , Zebrafish/genetics , Zebrafish Proteins/genetics
4.
Eur J Biochem ; 271(2): 339-48, 2004 Jan.
Article in English | MEDLINE | ID: mdl-14717701

ABSTRACT

We have cloned and determined the nucleotide sequence of the cDNA coding for a cellular retinol-binding protein type I (CRBPI) from zebrafish. The deduced amino acid sequence of the zebrafish CRBPI showed highest sequence identity ( approximately 59%) to the mammalian CRBPIs of the intracellular lipid-binding protein (iLBP) multigene family. Phylogenetic analysis clustered the zebrafish CRBPI to the CRBPI clade. The zebrafish CRBPI gene (rbp1) and CRBPII gene (rbp2) both consist of four exons separated by three introns, identical to all other iLBP genes in vertebrates. Two transcription start sites were identified in the rbp1 promoter and a single transcription start site was identified for rbp2. Radiation hybrid mapping assigned the zebrafish rbp1 gene to linkage group 16 and conserved syntenic genes were found by comparative analysis of mammalian orthologous rbp1 genes. RT-PCR detected mRNA transcripts in the adult intestine, liver, brain, ovary and testis for rbp1 gene and in the intestine and liver for rbp2 gene. Whole mount in situ hybridization of zebrafish embryos revealed rbp1 mRNA expression in the developing zebrafish central nervous system at specific sites that are known to have abundant retinoic acid distribution and significant retinoic acid action. Whole mount in situ hybridization also showed that the zebrafish rbp2 mRNA was localized specifically in the embryonic intestinal bulb and the developing intestine during the larval stage, implying a novel function for the rbp2 gene product during organogenesis and development of the zebrafish intestine.


Subject(s)
Gene Expression Regulation, Developmental , Retinol-Binding Proteins/genetics , Zebrafish Proteins/genetics , Zebrafish/embryology , Zebrafish/genetics , Amino Acid Sequence , Animals , Base Sequence , Chromosome Mapping , Conserved Sequence , DNA, Complementary/chemistry , DNA, Complementary/genetics , Genetic Linkage , In Situ Hybridization , Lipid Metabolism , Molecular Sequence Data , RNA, Messenger/metabolism , Radiation Hybrid Mapping , Retinol-Binding Proteins/metabolism , Retinol-Binding Proteins, Cellular , Reverse Transcriptase Polymerase Chain Reaction , Sequence Homology, Amino Acid , Transcription Initiation Site , Transcription, Genetic , Tretinoin/metabolism , Zebrafish/metabolism , Zebrafish Proteins/metabolism
5.
Dev Dyn ; 228(3): 386-92, 2003 Nov.
Article in English | MEDLINE | ID: mdl-14579377

ABSTRACT

We have used whole-mount in situ hybridization to analyze Na,K-ATPase alpha and beta subunit gene expression in the developing zebrafish ear. Four alpha1-like (alpha1a.1, alpha1a.2, alpha1a.4, and alpha1a.5) and two beta (beta1a and beta2b) subunit genes are expressed in ear beginning at mid-somitogenesis. Each gene exhibits a distinct spatial and temporal expression pattern. The alpha1a.1 gene was ubiquitously expressed in the otic epithelium from mid-somitogenesis to 24 hr postfertilization (hpf). Expression of this gene was gradually reduced and by 48 hpf, alpha1a.1 transcripts were no longer detectable in the ear. The alpha1a.2 and alpha1a.5 genes were expressed in regions that correspond to the anterior macula, lateral crista, and semicircular canal projections up to 48 hpf. At later stages, expression of these genes was limited to cells in the dorsolateral septum and semicircular canal projections. alpha1a.4 and beta1a transcripts were ubiquitously expressed during ear development and were present in most otic tissues at 5 days postfertilization (dpf). Expression of the beta2b gene, on the other hand, was restricted to subsets of cells that form sensory epithelia. These results strongly suggest different functional roles for individual Na,K-ATPase genes in zebrafish ear development. Na,K-ATPase genes are likely to represent useful markers for the analysis of zebrafish otogenesis.


Subject(s)
Ear, Inner/embryology , Sodium-Potassium-Exchanging ATPase/genetics , Zebrafish/embryology , Animals , Body Patterning , Ear, Inner/enzymology , Morphogenesis , Protein Subunits/genetics , Zebrafish Proteins/genetics
6.
Gene Expr Patterns ; 3(4): 525-32, 2003 Aug.
Article in English | MEDLINE | ID: mdl-12915322

ABSTRACT

Selenium is important for embryogenesis in vertebrates but little is known about the expression patterns and biological functions of most selenoprotein genes. Taking advantage of the zebrafish model, systematic analysis of selenoprotein gene expression was performed by in situ hybridization on whole-mount embryos at different developmental stages. Twenty-one selenoprotein mRNAs were analyzed and all of them exhibited expression patterns restricted to specific tissues. Moreover, we demonstrated that highly similar selenoprotein paralogs were expressed within distinct territories. Therefore, tissue- and development-specific expression patterns provided new information for selenoproteins of unknown function.


Subject(s)
Proteins/genetics , Zebrafish/embryology , Animals , Gene Expression Regulation, Developmental , In Situ Hybridization , Molecular Sequence Data , Proteins/metabolism , RNA Probes , RNA, Messenger/metabolism , Selenoproteins , Tissue Distribution , Zebrafish Proteins
7.
Gene Expr Patterns ; 2(3-4): 243-6, 2002 Dec.
Article in English | MEDLINE | ID: mdl-12617808

ABSTRACT

We have identified and characterized cDNAs encoding a novel zebrafish Na,K-ATPase alpha subunit. The full-length cDNA encodes a 1,023-amino-acid-long peptide which shows greatest homology to zebrafish alpha1 polypeptides. Radiation hybrid mapping localized the new gene (atp1a1a.5) to linkage group 1 in close proximity to the previously identified cluster of Na,K-ATPase alpha1 genes. The expression of atp1a1a.5 in zebrafish embryos was analyzed using whole-mount in situ hybridization. From mid-somitogenesis through 48 h post fertilization (hpf), atp1a1a.5 transcripts were detected in the pronephric duct, ear, and mucous cells. This expression pattern continues through 108 hpf, when high levels of expression were also detected in the intestinal bulb.


Subject(s)
Chromosome Mapping , Gene Expression Profiling , Sodium-Potassium-Exchanging ATPase/genetics , Zebrafish/genetics , Animals , Kidney/embryology , Kidney/metabolism , Organ Specificity , Zebrafish/embryology , Zebrafish Proteins
8.
Mech Dev ; 119 Suppl 1: S211-4, 2002 Dec.
Article in English | MEDLINE | ID: mdl-14516687

ABSTRACT

We have identified and characterized cDNAs encoding a novel zebrafish Na,K-ATPase alpha subunit. The full-length cDNA encodes a 1023-amino-acid-long peptide which shows greatest homology to zebrafish alpha1 polypeptides. Radiation hybrid mapping localized the new gene (atp1a1a.5) to linkage group 1 in close proximity to the previously identified cluster of Na,K-ATPase alpha1 genes. The expression of atp1a1a.5 in zebrafish embryos was analyzed using whole-mount in situ hybridization. From mid-somitogenesis through 48 h post fertilization (hpf), atp1a1a.5 transcripts were detected in the pronephric duct, ear, and mucous cells. This expression pattern continues through 108 hpf, when high levels of expression were also detected in the intestinal bulb.


Subject(s)
Sodium-Potassium-Exchanging ATPase , Zebrafish , Amino Acid Sequence , Animals , Cloning, Molecular , In Situ Hybridization , Molecular Sequence Data , Zebrafish/genetics
SELECTION OF CITATIONS
SEARCH DETAIL
...