ABSTRACT
INTRODUCTION: Fruits of oleaster (Elaeagnus angustifolia L.) were used in traditional medicine to treat various diseases. The aim of this study was to evaluate and compare the phenol and flavonoid contents and antioxidant activity of methanol extracts from the fruit peel, flesh and seed of seven genotypes of oleaster. METHODS: The phenol and flavonoid contents were determined using spectrophotometric methods. Antioxidant and antiradical activities were determined using reducing power, ferric-reducing antioxidant potential (FRAP) and ability to scavenge DPPH radical assays. RESULTS: Significant differences ( P < 0.05) were found in phenol and flavonoid contents and antioxidant activity among components of fruit and within various genotypes. CONCLUSION: RESULTS indicated that oleaster has good fruit quality varying among different genotypes. Seeds of fruits have excellent antioxidant activity and phenolic contents in comparison to flesh and peel.
ABSTRACT
In this study, the catalytic activity of aldehyde oxidase was investigated in various water-miscible organic solvents for the first time. The enzyme was partially purified from rabbit liver, and its activity was determined in the absence/presence of nine hydro-organic mixtures. The effects of pH and temperature on the enzyme activity were also investigated. The activity was reduced in the presence of all nine organic solvents. However, in some cases, the residual activity remained almost unchanged throughout the incubation of enzyme at 35 °C for 24 h. Considering potential advantages of doing reactions in the presence of organic solvents, these results could be of value. The enzyme showed different behavior in the reaction solutions making it difficult to formulate results in a single comprehensive model. The results indicated that binding and cleavage of the substrate are influenced in the presence of organic solvents.
