Thermal changes in cow's mammary gland after experimentally invoked inflammation (mastitis).

The purpose of the study was the evaluation of the thermal changes within mammary gland in cows, after experimentally invoked inflammation, developed as a result of 60 ml of 20% NaCl solution given directly into the udder (A and D quarters). Three hours after injection, the increased' temperature of teats and their bases by 2.26 degrees C and 0.58 degree C, respectively, was stated. The temperature was still increased after 6 hours from NaCl solution injection. It was accompanied by increase of heat flux from the skin surface by 50.17 mW/cm2 taken from the teat surface, and by increased values of Burton Index--the tissue blood supply index.

The effect of PGF2 alpha-estrumate on the secretion and composition of abomasal juice in sheep during H2-histaminic receptor blockade with cimetidine.

The effect of PGF2 alpha was investigated on the secretion of abomasal juice in sheep after blockade of the H2-histaminic receptor with intravenous cimetidine (150-200 mg). Cimetidine inhibited abomasal juice secretion diminishing juice volume and the content of acid and pepsin in it. During this inhibition intramuscular injection of PGF2 alpha 2.0-4.0 micrograms/kg of body weight caused an increase of these parameters. In control experiments histamine administered (23-28 micrograms/kg) after cimetidine failed to increase the secretion of abomasal juice confirming thus complete blockade of the H2-histaminic receptor. The obtained results demonstrated that the stimulating effect of PGF2 alpha on abomasal juice secretion was not mediated by the H2-histaminic receptor.

Comparison of the effects of two prostaglandin F2 alpha analogues-estrumate and lutalyse on the volume, activity, and proteolytic power of the abomasal juice in sheep.

The investigations were carried out on 7 wethers with cannulated fistulas of the "small" abomasum and rumen. In two series of experiments the optimal dose of one of the tested analogues of PGF 2 alpha (Estrumate 3-4 microgram/kg, Lutalyse 130-170 microgram/kg of body weight) was used. It was found the Estrumate in relation to Lutalyse caused a highly statistical significant rise (P less than 0.01) in the volume of secreted juice, free HCl, and total acidity in the same sheep. The specific activity (Anson mU/ml) increased more after Lutalyse (P less than 0.01) than after Estrumate injection. This analogue also caused significant (P less than 0.02) changes in the total activity (specific activity X juice volume of this sample). The obtained results expressed in per cent indicate that Estrumate exerts a greater effects on the parietal cells in the abomasal mucosa in sheep and Lutalyse stimulates more strongly the chief cells, as reflected in increased proteolytic power of the studied juice.

[Effect of stress on uterine contraction in cows]. / Wplyw stresu na motoryke macicy u krów.

The investigation was carried out to observe the influence of stress upon the motility of the uterus from 1st to 5th day after parturition or cesarotomy. The experiments involved 24 lowland black and white cows; in 12 of them cesarean section was performed. The motility of the uterus was recorded with the balloon method by way of air transmission. The starting uterine contractions curve having been recorded, for 15-20 minutes one of following stress agents was applied: taking the calf away, change of stall, putting under restraint, collecting small amounts of blood or milking by a strange person. Besides, 2 ml 0.1% adrenaline solution or 4 I.U. oxytocin was administered intravenously. Adrenaline was administered before or after oxytocin, or both the hormones were applied together. Oxytocin was also applied during the stress. During the action of the stress as well as after administration of adrenaline there was observed complete decay of uterine contractions from 1st to 5th day after parturition or cesarotomy. No changes in the motility of the uterus were brought about by intravenously administration of oxytocin during the stress. No response of the uterus to oxytocin was observed, either, when adrenaline had been administered before. Both exogenic and endogenic adrenaline was found to restrain strongly the contractions of the examined organ, or to decay them completely, which has a negative effect upon involution of the uterus in cows in the first days after parturition.

Investigations of the effect of F2 alpha-prostaglandin--lutalyse on the volume of sheep abomasal juice, its acidity and proteolytic power.

In the experiments carried out on 3 sheep (males) it was demonstrated that intramuscular administration of Lutalyse in optimal doses (130-170 g/kg of body weight) caused a rise in the volume of juice, the amount of free HCl, and the value of total acidity, as well as an increase in the specific activity (Anson's milliunits in ml) and total activity (specific activity multiplied by juice volume in each sample). It was found that Lutalyse (an analogue of PGF2 alpha) increased the secretory activity of the glandular cells of the abomasum and stimulated particularly powerfully the chief cells, with consequent rise in the proteolytic power of the juice.

Effect of synthetic prostaglandin PGF2 alpha on the volume, acidity, and proteolytic activity of the abomasum juice in sheep.

Effect of synthetic prostaglandin PGF2 alpha on the volume acidity, and proteolytic activity of the abomasum juice in sheep. Act Physiol. Pol., 1979, 30 (2): 299--304. The investigations were carried out in 3 male sheep on the effect of Estrumate (a synthetic analogue of PGF2 alpha) on the secretion and composition of juice from the "small" abomasum. After intramuscular injection of optimum doses of the preparation (3.0--4.0 micrograms/kg of body weight) it was observed that the volume of the juice, its free HCl content, the total acidity the specific activity (expressed in Anson milliunits/ml) and the total activity (specific activity X volume of juice secreted in a given sample) increased significantly (p less than 0.01). Thus, the synthetic prostaglandin PGF2 alpha stimulates the secretory activity of abomasum glands in sheep.

Pharmacological analysis of the abomasum-rumen reflex in sheep.

Contractions of the dorsal sac of the rumen and abomasum were recorded in the sheep with chronic fistulae. It was found that during reflex inhibition of rumen contractions, induced with stimulation of chemoreceptors in the abomasum, an increased sympathicotonia plays an essential role and the beta-adrenergic receptors mediate the reflex.