Elephantoloemus indicus Austen, 1930 (Diptera: Calliphoridae) as the cause of cutaneous myiasis in captive Indian elephants from Assam, India.

Elephantoloemus indicus Austen, 1930, a dipteran calliphorid fly is known to cause by its larval stage obligatory cutaneous myiasis in Indian subspecies of Asian elephants (Elephas maximus indicus Cuvier, 1798) in Myanmar and Thailand. The present study was undertaken on morphological identification of some specimens of fly larvae which were recovered from the warbles detected on the skin of captive Indian elephants at the Nameri National Park and Kaziranga National Park both situated in the state of Assam, India. The larval specimens were whitish to creamy white in colour and body conformation varied from cylindrical to barrel shaped depending on their measured size (Av 6.12 ± 0.28 × 2.35 ± 0.12 mm). Microscopic examination of processed larvae revealed presence of numerous single pointed spines uniformly distributed on entire body surface, well developed mouth hooks and cephalopharyngeal skeleton at the anterior end and posterior spiracles each with lightly sclerotized peritreme enclosing three short and straight respiratory slits. Based on geographical distribution of the fly, host relation, larval parasitism and morphological characters, the larvae were determined as of the genus Elephantoloemus which is represented by E. indicus as the only species described so far. This finding seems to be the first record in India after its report from Myanmar and Thailand.

Occult Dirofilariosis in Dogs of North Eastern Region in India.

BACKGROUND: The North Eastern Region in India is endemic for canine heartworm disease but in clinics accurate diagnosis is some times difficult. The aim of the present study was to determine the prevalence of occult infections for heartworm disease in canine in two geographical regions of North Eastern India. METHODS: A total of 782 numbers of three categories of dogs namely, working dogs of military and paramilitary forces, pet dogs and stray dogs were screened for the presence of heartworm infection from August 2011 to July, 2012 in Guwahati (Assam) and Aizawl (Mizoram). Conventional, immunological and molecular techniques were followed for this epidemiological study. The criteria to determine the occult heartworm cases were based on the differences between heartworm positive cases in PCR test and antigen ELISA test. RESULTS: The findings revealed an overall 22.69 percent occult case. The working dogs had highest prevalence (60%) followed by pet (29.16%) and stray dogs (17.75%). CONCLUSION: The highest percentage of occult heartworm infection was present in working dogs maintained under military or paramilitary forces.

Prevalence and molecular epidemiological data on Dirofilaria immitis in dogs from Northeastern States of India.

The aim of the present study was to determine the prevalence of Dirofilaria immitis in stray, pet, and working dogs (n = 413, 266, and 103, resp.) from Guwahati (Assam) and Aizawl (Mizoram), areas located in two Northeastern States of India. Diagnostic methods applied were microscopy (wet film and Knott's concentration technique), immunological test (Ag ELISA by SNAP 4Dx ELISA kit), and molecular tools (polymerase chain reaction and sequencing), which evidenced 11.38, 18.03, and 13.93% of positive animals, respectively. No significant differences were observed by area (18.23% versus 17.68%) nor by sex (18.1% versus 17.9%), whereas stray dogs proved more infected than other groups (P < 0.05). ELISA test evidenced an overall 22.69% of occult infections, mainly in working dogs (60%), and molecular techniques detected Dirofilaria (Nochtiella) repens in 4 stray dogs from Guwahati. Characterization of D. immitis isolates for ITS-2 region showed close identity with South Asian isolates.