Drift-reducing nozzles and their biological efficacy.

In 2007 and 2008, field trials were carried out with different standard and drift-reducing nozzles in sugar beet, maize, chicory, Belgian endive (all herbicide applications), wheat (fungicide application) and potatoes (Haulm killing herbicide application). The effect of nozzle type (standard flat fan, low-drift flat fan, air injection), nozzle size (ISO 02, 03 and 04) and application volume on the biological efficacy was investigated. All applications were done using a plot sprayer with volume rates ranging from 160 to 320 l.ha(-1) at recommended dose rates with commonly used (mix of) plant protection products. For each crop, the experiments included four replicates in a randomized block design. Depending on the type of application, the efficacy was measured in terms of weed control, disease and yield level, percentage dead leaf and stem, etc. In a previous research, drift and droplet characteristics of the different techniques were measured. In general no important effect of application technique on biological efficacy was observed for the tested herbicide and fungicide applications within the interval of volume rates and droplet size tested. Drift-reducing nozzles performed similar as conventional nozzles under good spraying conditions and using a correct spray application technique.

Interception of spray drift by border structures. Part 1: wind tunnel experiments.

This research investigated the drift-intercepting potential of structures surrounding the field borders, like artificial screens and crops, which are not yet a part of the drift mitigation measures for field crop sprayers in Belgium. Drift-interception experiments were performed in the wind tunnel of the International Centre for Eremology (Ghent University, Belgium) with various interception structures: Artificial screens with heights of 0.5, 0.75 and 1 m and screen open areas of 16, 36 and 63%; a row of plastic Christmas trees with heights of 0.5 and 0.75 m; and a potato canopy. The interception structure was positioned at 1 m from the field border. From the results it was found that type of border structure has a pronounced effect on the drift interception, while the height of the border structure had no significant effect.

Interception of spray drift by border structures. Part 2: field experiments.

This research studied the effect of drift-intercepting structures surrounding the field borders, like artificial screens and natural hedges, which are not yet a part of the drift mitigation measures for field crop sprayers in Belgium. Drift-interception experiments were performed in a grassland (Lolium perenne) with various interception structures: Artificial screens with heights of 1, 1.5 and 2 m and screen open areas of 16, 36 and 63% and a row of Fagus sylvatica trees with a height of 1.5 m and an average leaf area index of 1.12 m2/m2. Experiments were performed according to the international standard ISO 22866. The interception structure was positioned at 1 m from the field border. From the results it was found that type of border structure as well as screen open area and screen height, have an important effect on the amount of spray drift. Highest drift reduction was found with a 1.5 m artificial screen with a 16% open area.

Occurrence of spray drift for different crop types: cereal, cereal stubble and grassland.

Pesticide spray drift is affected by 4 main factors: weather conditions, spray application technique, physicochemical properties of the spray Liquid and surrounding characteristics. This research studied the importance of crop type being sprayed for drift occurrence. Drift experiments were performed over cereals, cereal stubbles and grassland according to the international standard ISO 22866. From the results it was found that drift occurrence in cereals and cereal stubbles was lower than drift occurrence in grassland. The differences between cereals and cereal stubbles were significant only at low wind speed.

Direct and indirect drift assessment means. Part 1: PDPA laser based droplet characterisation.

The spray quality generated by agricultural nozzles is important considering the efficiency of the pesticide application process because it affects spray deposits, biological efficacy and driftability. That is why a measuring set-up for the characterisation of spray nozzles was developed. This set-up is composed of a controlled climate room, a spray unit, a three-dimensional automated positioning system and an Aerometrics PDPA laser system which measures droplet size and velocity characteristics based on light scattering principles. Using this set-up and a well defined measuring protocol, droplet size and velocity characteristics of 15 different nozzle-pressure combinations were measured. It was found that at a nozzle distance of 0.50 m, droplet sizes vary from a few up to some hundreds of micrometres and droplet velocities from about 0 m.s(-1) up to 16 m.s(-1). From the results, the importance of the nozzle type and size on the droplet size and velocity spectra is clear. Standard flat fan nozzles produced the finest droplet size spectrum followed by low-drift and the air injection nozzles which results in significant differences in the proportion of small droplets. The larger the ISO nozzle size, the coarser is the droplet size spectrum and the lower is the proportion of small droplets. This effect is most pronounced for the standard flat fan followed by the low-drift nozzles and is less important for the air inclusion nozzles. Comparing the PDPA measuring results with other studies confirmed the need for reference nozzles to classify sprays because of the considerable variation of absolute results depending on variations in reference sprays, measuring protocol, measuring equipment and settings. As described in part 4, these results will be linked with the drift potential of different nozzle-pressure combinations.

Direct and indirect drift assessment means. Part 3: field drift experiments.

A whole series of field drift experiments were performed to investigate the effect of nozzle type (flat fan, low-drift, air inclusion) and size (ISO 02, 03, 04 and 06) on sedimenting spray drift. Sedimenting spray drift was determined by sampling in a downwind area at 24 different positions using horizontal drift collectors in combination with a fluorescent tracer with measurements up to 20 m from the directly sprayed zone. Meteorological conditions were continuously monitored. Based on 27 drift experiments with the reference spraying at various environmental conditions, the important effect of atmospheric conditions on the amount of near-field sedimenting spray drift was demonstrated and quantified. A non-linear drift prediction equation was set up and validated. This equation was used to compare the drift results of the different spraying techniques under various weather conditions with the reference spraying by calculating their total drift reduction potential (DRPt). Air inclusion nozzles have the highest drift reduction potential followed by the low-drift nozzles and the standard flat fan nozzles and the effect on drift deposits is high with DRPt values varying from -136.5 up to 89.8%. The effect of nozzle type is most important for smaller nozzle sizes A large database with (absolute) near-field drift results is made available to enlarge the international drift database with information about the effect of climatological conditions and spray application technology. The results are generally in good agreement with the results from different other studies although drift studies are difficult to compare due to differences in weather conditions, spray application techniques, methodologies and crop conditions.

The effect of air support on droplet characteristics and spray drift.

Air assistance on field sprayers creates a forced airstream under the spray boom which blows the spray droplets into the crop. The advantages of this relative new technique are less drift of spray droplets and the possibility to reduce the amount of pesticides and spray Liquid. The purpose of this work was to investigate the effect of air assistance on the characteristics of spray droplets and their driftability. Based on air velocity measurements on an air assisted field sprayer, a system of air assistance was developed in addition to a laser-based measuring set-up for the characterisation of spray droplets. With this set-up, the effect of air support on the droplet characteristics was investigated for different settings of the air assistance. The effect on spray drift was quantified based on field drift measurements. A reducing effect on the total amount of spray drift was demonstrated for the Hardi ISO F 110 02, F 110 03 and LD 110 02 nozzles with drift reduction factors a(d) of, respectively, 2.08, 1.77 and 1.53. The use of air support had no significant effect for the LD 110 03 nozzles on the total amount of spray drift. Comparing droplet size and drift results, it was found that air support has the highest impact on the amount of spray drift for the finer sprays by increasing droplet velocities. The effect of air support on droplet sizes is rather limited.

Fluorimetric determination of aflatoxin M1 in cheese.

A simple and sensitive method is proposed for the determination of aflatoxin M1 in cheese. The ground cheese sample is extracted with acetone-water (3 + 1). Acetone is evaporated under vacuum, and the aqueous phase is passed through a C18 disposable cartridge. After the cartridge is washed with acetonitrile-water (1 + 9), the toxin is eluted with acetonitrile. The extract is then cleaned up on a silica cartridge. Final analysis is performed by 2-dimensional thin layer chromatography (TLC) combined with fluorodensitometry or by liquid chromatography on a reverse phase C18 column with fluorescence detection. Recovery is greater than 90%, and the coefficient of variation is 6% or less. The detection limit is in the range of 10 ng/kg. The identity of aflatoxin M1 is confirmed by formation of the M2a or acetyl-M1 derivative and rechromatography.

Systematic identification of antioxidants in lards, shortenings, and vegetable oils by thin layer chromatography.

A simple and reliable method is described for rapid identification of ascorbyl palmitate, butylated hydroxyanisole, butylated hydroxytoluene, ethoxyquin, gallates (lauryl, octyl, propyl), nordihydroguaiaretic acid, 3,3'-thiodipropionic acid, tocopherol, t-butylhydroquinone, and 2,4,5-trihydroxybutyrophenone in lards, shortenings, and vegetable oils. The antioxidants are extracted with 95% methanol, concentrated under vacuum at less than or equal to 45 degrees C, and analyzed by thin layer chromatography. Three elution solvents, 2 adsorbent types, 2 visualization sprays, and UV viewing at 254 and 36 nm are used. Sunflower and corn oil samples, fortified with 100 ppm antioxidant, were analyzed to establish to validity of the method.