Subject(s)
Arthritis, Psoriatic/drug therapy , Etanercept/adverse effects , Isoniazid , Mycobacterium tuberculosis/isolation & purification , Tuberculosis, Lymph Node , Antitubercular Agents/administration & dosage , Antitubercular Agents/classification , Chemoprevention/adverse effects , Chemoprevention/methods , Etanercept/administration & dosage , Humans , Immunosuppressive Agents/administration & dosage , Immunosuppressive Agents/adverse effects , Isoniazid/administration & dosage , Isoniazid/adverse effects , Male , Middle Aged , Risk Adjustment , Secondary Prevention/methods , Secondary Prevention/standards , Tuberculosis, Lymph Node/immunology , Tuberculosis, Lymph Node/physiopathology , Tuberculosis, Lymph Node/therapyABSTRACT
Not available.
Subject(s)
HIV Seropositivity/complications , Latent Tuberculosis/complications , Latent Tuberculosis/drug therapy , Humans , ItalyABSTRACT
Background: To improve our understanding of the natural history of Zika virus (ZIKV) infection in humans, we described the dynamics of ZIKV RNA shedding in different body fluids and antibody responses in patients with acute infection. Methods: Twenty-nine adults with travel-associated infection and 1 case of sexual transmission were enrolled and followed up with weekly ZIKV RNA testing in blood, urine, saliva, and semen samples and antibody testing. Results: ZIKV RNA was detected in plasma, urine, and saliva of 57%, 93.1%, and 69.2% of participants, with estimated median times to clearance of 11.5 days (interquartile range [IQR] 6-24 days), 24 days (IQR, 17-34), and 14 days (IQR, 8-31), respectively. In 2 pregnant women, ZIKV RNA persisted in blood until delivery of apparently healthy infants. ZIKV RNA was detected in semen of 5 of 10 tested men; median time to clearance was 25 days (IQR 14-29), and the longest time of shedding in semen was 370 days. In flavivirus-naive patients, the median times to detection of ZIKV nonstructural protein 1 (NS1)-specific immunoglobulin M (IgM) and immunoglobulin G (IgG) antibodies were estimated as 8 days (IQR, 5-15 days) and 17 days (IQR, 12-26 days), respectively. ZIKV NS1 IgM antibodies were undetectable in patients with previous dengue. Conclusions: Prolonged viremia and ZIKV RNA shedding in urine, saliva, and semen occur frequently in patients with acute ZIKV infection. At the time of diagnosis, about half of patients are ZIKV IgM negative. ZIKV NS1 IgM antibodies remain undetectable in patients with previous dengue. Estimates of the times to viral clearance and seroconversion are useful to optimize diagnostic algorithms.
Subject(s)
Antibodies, Viral/blood , Zika Virus Infection/virology , Zika Virus/immunology , Acute Disease , Adolescent , Adult , Female , Follow-Up Studies , Humans , Immunoglobulin G/blood , Immunoglobulin M/blood , Infant , Italy , Male , Middle Aged , Pregnancy , RNA, Viral/blood , RNA, Viral/urine , Saliva/virology , Semen/virology , Travel , Viremia , Virus Shedding , Young Adult , Zika Virus/genetics , Zika Virus/physiology , Zika Virus Infection/diagnosis , Zika Virus Infection/immunologyABSTRACT
OBJECTIVES: The incidence of HIV and syphilis among men who have sex with men (MSM) in Europe has recently increased. Rapid point-of-care tests (POCTs) for syphilis can improve access to screening. The purpose of this study was to evaluate the performance of two syphilis POCTs compared with laboratory tests among MSM. METHODS: The study was undertaken in Verona, Italy. Asymptomatic MSM, potentially exposed to syphilis, were enrolled prospectively. The POCTs evaluated were SD Bioline Syphilis 3.0 and Chembio DPP Syphilis Screen & Confirm Assay on both serum and fingerprick blood. The results of the POCTs were read by the naked eye by two independent readers and their concordance assessed. RESULTS: A total of 289 MSM were enrolled in the study. Based on laboratory tests, 35 MSM (12.1%) were TPPA-positive alone and 16 (5.5%) were both Treponema pallidum particle agglutination test (TPPA) and rapid plasma reagin (RPR)-positive. The specificities of both POCTs were above 99% on both serum and fingerstick blood specimens, while sensitivities varied considerably. The sensitivity of the SD Bioline test was lower on fingerprick blood (51.4% and 54.3%, readers 1 and 2, respectively) compared with that on serum (80.0% and 82.9%). In contrast, the Chembio test exhibited similar sensitivity values for serum and fingerprick samples (57.7% and 64.0% on serum vs 65.4% and 69.2% on fingerprick for the treponemal component; 63.6% on both samples by both readers for the non-treponemal component). The positive predictive value ranged between 100% and 93.9% for the treponemal component of both syphilis POCTs, but was lower (76.3%-100%)%) for the non-treponemal component of the Chembio POCT. The negative predictive value surpassed 90% for both tests on both samples. The agreement between readers was very high (>99%). CONCLUSION: The diagnostic performance of the syphilis POCTs was lower than expected; however, considering the prevalence of syphilis among MSM, POCTs should be recommended to improve syphilis detection among MSM.
Subject(s)
Point-of-Care Systems , Point-of-Care Testing , Sexual and Gender Minorities , Syphilis/diagnosis , Treponema pallidum/isolation & purification , Adult , Antibodies, Bacterial/analysis , Humans , Italy/epidemiology , Male , Middle Aged , Prospective Studies , Sensitivity and Specificity , Syphilis/microbiology , Syphilis/transmissionABSTRACT
Although disseminated Mycobacterium avium complex disease occurs mainly in immunocompromised hosts, especially HIV-infected patients in the last stage of the disease (AIDS), this condition is still rare in immunocompetent subjects. We report the case of a Caucasian man who received a left ventricular assist device two years before as a bridge to heart transplantation, that began to present signs and symptoms of mycobacterial infection. The diagnostic work-up we performed showed the presence of Mycobacterium intracellulare in lungs and both peripherical and bone marrow blood. Although evaluated, we found no abnormalities in the patient's immune system that can be related to mycobacterial infection. The beginning of a specific therapy made the patient slowly improve and further nuclear medicine assay (PET-TC) showed a good reduction in radio-labelled drug captation.
Subject(s)
Heart-Assist Devices , Mycobacterium avium Complex/isolation & purification , Mycobacterium avium-intracellulare Infection/diagnosis , Mycobacterium avium-intracellulare Infection/microbiology , Antitubercular Agents/therapeutic use , Drug Therapy, Combination , Fatal Outcome , Heart Failure , Heart-Assist Devices/adverse effects , Humans , Immunocompromised Host , Male , Middle Aged , Mycobacterium avium Complex/drug effects , Mycobacterium avium-intracellulare Infection/drug therapyABSTRACT
We describe a case of symptomatic acute HIV infection in a young man where a fourth-generation rapid screening test combining HIV-specific antibody and p24 antigen was negative. In highly suspicious cases of acute HIV infection, plasma HIV RNA assays together with conventional, non-rapid screening tests should be used.
