ABSTRACT
This corrects the article DOI: 10.1038/onc.2016.209.
ABSTRACT
Canonical Wnt signaling induces the stabilization of ß-catenin, its translocation to the nucleus and the activation of target promoters. This pathway is initiated by the binding of Wnt ligands to the Frizzled receptor, the association of the LRP5/6 co-receptor and the formation of a complex comprising Dvl-2, Axin and protein kinases CK1α, É, γ and GSK3. Among these, activation of CK1É, constitutively bound to LRP5/6 through p120-catenin, is required for the association of the rest of the components. We describe here that CK1É is activated by the PP2A/PR61É phosphatase. Binding of Wnt ligands promotes the interaction of LRP5/6-associated CK1É with Frizzled-bound PR61É regulatory subunit, facilitating the access of PP2A catalytic subunit to CK1É and its activation, what enables the recruitment of Dvl-2 to the receptor complex and the initiation of the Wnt pathway. Our results uncover the mechanism of activation of the canonical Wnt pathway by its ligands.
Subject(s)
Casein Kinase Idelta/metabolism , Protein Phosphatase 2/metabolism , Frizzled Receptors/metabolism , HEK293 Cells , HT29 Cells , Humans , Wnt Signaling PathwayABSTRACT
Snail1 is a transcriptional factor essential for triggering epithelial-to-mesenchymal transition. Moreover, Snail1 promotes resistance to apoptosis, an effect associated to PTEN gene repression and Akt stimulation. In this article we demonstrate that Snail1 activates Akt at an additional level, as it directly binds to and activates this protein kinase. The interaction is observed in the nucleus and increases the intrinsic Akt activity. We determined that Akt2 is the isoform interacting with Snail1, an association that requires the pleckstrin homology domain in Akt2 and the C-terminal half in Snail1. Snail1 enhances the binding of Akt2 to the E-cadherin (CDH1) promoter and Akt2 interference prevents Snail1 repression of CDH1 gene. We also show that Snail1 binding increases Akt2 intrinsic activity on histone H3 and have identified Thr45 as a residue modified on this protein. Phosphorylation of Thr45 in histone H3 is sensitive to Snail1 and Akt2 cellular levels; moreover, Snail1 upregulates the binding of phosphoThr45 histone H3 to the CDH1 promoter. These results uncover an unexpected role of Akt2 in transcriptional control and point out to phosphorylation of Thr45 in histone H3 as a new epigenetic mark related to Snail1 and Akt2 action.
