Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 7 de 7
Filter
Add more filters










Database
Publication year range
1.
Eur J Clin Chem Clin Biochem ; 29(11): 759-65, 1991 Nov.
Article in English | MEDLINE | ID: mdl-1782284

ABSTRACT

Some aspects of the measurement of alkaline phosphatase activity concentration in human serum, using N-methyl-D-glucamine as a buffer, were evaluated with a view to the possible routine use of the method. The evaluated characteristics included: the temperature-dependence of the pH of the buffer; the effect of adding the magnesium/zinc ions buffer; the effect of the serum volume fraction; the substrate-starter versus the serum-starter mode; the effect of modifying the formulation of reagents; the within-run and the between laboratory imprecision; the correlation with an alternative routine method. Also, sex- and age-related reference values were produced, based on 2968 values from selected reference sample groups in 7 laboratories. In general, the results demonstrate the robustness of the method, its adaptability to a variety of mechanized analysers, and hence its feasibility as a routine measurement method.


Subject(s)
Alkaline Phosphatase/blood , Meglumine , Adolescent , Adult , Aged , Aged, 80 and over , Buffers , Child , Child, Preschool , Evaluation Studies as Topic , Female , Humans , Hydrogen-Ion Concentration , Infant , Infant, Newborn , Magnesium/pharmacology , Male , Methods , Middle Aged , Reference Values , Reproducibility of Results , Sensitivity and Specificity , Substrate Specificity , Temperature , Zinc/pharmacology
4.
Quad Sclavo Diagn ; 14(2): 259-65, 1978 Jun.
Article in Italian | MEDLINE | ID: mdl-751101

ABSTRACT

A rapid and simple procedure measuring the rate of hemolysis of 50% of erytrocytes suspended in a standardized glycerol mixture (GLT/50) has been compared with the erytrocyte osmotic fragility to NaCl 0.375% concentration, carried out according to Parpat's method modified, in order to estimate the respective behaviour in the screening of beta-thalassemia trait. The GLT/50 is provided with more selectivity especially between beta-thalassemia trait carriers and other subjects with iron deficiency anemia.


Subject(s)
Glycerol , Osmotic Fragility , Thalassemia/diagnosis , Anemia, Hypochromic/diagnosis , Humans , Mass Screening
SELECTION OF CITATIONS
SEARCH DETAIL
...