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1.
Viruses ; 13(8)2021 07 28.
Article in English | MEDLINE | ID: mdl-34452346

ABSTRACT

African swine fever (ASF) is a hemorrhagic disease of swine characterized by massive lymphocyte depletion in lymphoid tissues due to the apoptosis of B and T cells, a process likely triggered by factors released or secreted by infected macrophages. ASFV CD2v (EP402R) has been implicated in viral virulence and immunomodulation in vitro; however, its actual function(s) remains unknown. We found that CD2v expression in swine PK15 cells induces NF-κB-dependent IFN-ß and ISGs transcription and an antiviral state. Similar results were observed for CD2v protein treated swine PBMCs and macrophages, the major ASFV target cell. Notably, treatment of swine PBMCs and macrophages with CD2v protein induced apoptosis. Immunoprecipitation and colocalization studies revealed that CD2v interacts with CD58, the natural host CD2 ligand. Additionally, CD58 knockdown in cells or treatment of cells with an NF-κB inhibitor significantly reduced CD2v-mediated NF-κB activation and IFN-ß induction. Further, antibodies directed against CD2v inhibited CD2v-induced NF-κB activation and IFN-ß transcription in cells. Overall, results indicate that ASFV CD2v activates NF-κB, which induces IFN signaling and apoptosis in swine lymphocytes/macrophages. We propose that CD2v released from infected macrophages may be a significant factor in lymphocyte apoptosis observed in lymphoid tissue during ASFV infection in pigs.


Subject(s)
African Swine Fever Virus/metabolism , African Swine Fever/genetics , African Swine Fever/physiopathology , Interferon-beta/genetics , Leukocytes, Mononuclear/cytology , Viral Proteins/metabolism , African Swine Fever/immunology , African Swine Fever/virology , African Swine Fever Virus/genetics , Animals , Apoptosis , Interferon-beta/immunology , Leukocytes, Mononuclear/immunology , Leukocytes, Mononuclear/virology , Macrophages/immunology , Macrophages/virology , NF-kappa B/genetics , NF-kappa B/immunology , Swine , Viral Proteins/genetics
2.
Immun Inflamm Dis ; 5(4): 421-434, 2017 12.
Article in English | MEDLINE | ID: mdl-28597552

ABSTRACT

INTRODUCTION: Organ-specific autoimmune diseases are believed to result from immune responses generated against self-antigens specific to each organ. However, when such responses target antigens expressed promiscuously in multiple tissues, then the immune-mediated damage may be wide spread. METHODS: In this report, we describe a mitochondrial protein, branched chain α-ketoacid dehydrogenase kinase (BCKDk ) that can act as a target autoantigen in the development of autoimmune inflammatory reactions in both heart and liver. RESULTS: We demonstrate that BCKDk protein contains at least nine immunodominant epitopes, three of which, BCKDk 71-90, BCKDk 111-130 and BCKDk 141-160, were found to induce varying degrees of myocarditis in immunized mice. One of these, BCKDk 111-130, could also induce hepatitis without affecting lungs, kidneys, skeletal muscles, and brain. In immunogenicity testing, all three peptides induced antigen-specific T cell responses, as verified by proliferation assay and/or major histocompatibility complex class II/IAk dextramer staining. Finally, the disease-inducing abilities of BCKDk peptides were correlated with the production of interferon-γ, and the activated T cells could transfer disease to naive recipients. CONCLUSIONS: The disease induced by BCKDk peptides could serve as a useful model to study the autoimmune events of inflammatory heart and liver diseases.


Subject(s)
Autoimmune Diseases/immunology , Epitopes, T-Lymphocyte/immunology , Hepatitis, Autoimmune/immunology , Myocarditis/immunology , Protein Kinases/immunology , Amino Acid Sequence , Animals , Autoantigens/chemistry , Autoantigens/immunology , Autoimmune Diseases/pathology , Biopsy , Cytokines/metabolism , Disease Models, Animal , Epitopes, T-Lymphocyte/chemistry , Hepatitis, Autoimmune/pathology , Histocompatibility Antigens Class II/genetics , Histocompatibility Antigens Class II/immunology , Immunization , Mice , Myocarditis/pathology , Peptide Fragments/chemistry , Peptide Fragments/immunology , Protein Kinases/chemistry , Protein Multimerization , T-Lymphocytes/immunology , T-Lymphocytes/metabolism
3.
J Gen Virol ; 83(Pt 9): 2257-2267, 2002 Sep.
Article in English | MEDLINE | ID: mdl-12185281

ABSTRACT

Like other members of the alpha subfamily of herpesviruses, bovine herpesvirus type 1 (BHV-1) establishes latent infections in sensory neurons. BHV-1 induces apoptosis in lymphoid cells in vivo and in epithelial cell lines, but the ability of BHV-1 to induce apoptosis in sensory neurons remains unknown. In this report, the susceptibility of rabbit ganglionic neurons to infection by BHV-1 was examined in vitro and in vivo. Following infection of cultured neurons with BHV-1, hallmarks of apoptosis such as chromatin condensation, DNA fragmentation and membrane blebbing were detected. The appearance of these changes was preceded by active viral DNA replication as determined by in situ hybridization. When viral DNA replication was blocked by treatment of cultures with an inhibitor of eukaryotic DNA polymerases, apoptosis but not virus attachment to neurons or bICP0 gene expression was completely prevented. Taken together, these results demonstrate that sensory neurons are not intrinsically resistant to BHV-1-induced apoptosis and that viral DNA replication plays a role in triggering the apoptotic programme. Infection of rabbits with BHV-1 resulted in pathological changes in the trigeminal ganglia (TG) which included mononuclear cell infiltration and neuronophagia. Morphological evidence of apoptosis was not detected in neurons, even in cells with advanced cytophatology. Furthermore, whereas DNA fragmentation was common in infiltrating cells, it was very rare and sporadic in neurons. Therefore, mechanisms in the TG should exist to prevent neuronal apoptosis upon BHV-1 infection.


Subject(s)
Apoptosis , Herpesvirus 1, Bovine , Neurons, Afferent/pathology , Trigeminal Ganglion/pathology , Animals , Cells, Cultured , DNA, Viral/analysis , Disease Models, Animal , Herpesviridae Infections/pathology , Herpesviridae Infections/virology , Neurons, Afferent/virology , Rabbits , Trigeminal Ganglion/virology , Virus Replication
4.
Buenos Aires; Editorial Sudamericana Sur S.A; 1984. 89 p. ilus, tab, graf. (69103).
Monography in Spanish | BINACIS | ID: bin-69103
5.
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