Rib fractures prediction method for kinetic energy projectile impact: from blunt ballistic experiments on SEBS gel to impact modeling on a human torso FE model.

The present paper aims to assess the risk of rib fractures caused by any rigid less-lethal kinetic energy projectiles. To that end, a coupled experimental and numerical approach is proposed to relate ballistic experiments with the risk of blunt trauma. A polymer gel block is employed as ballistic testing medium to interpret ballistic impacts through the measurement of the dynamic gel wall displacement. Moreover, a biofidelic 50th percentile human torso finite element model created in the code Hypermesh (Altair HyperWorks ©) is used to replicate experiments and real world accidents. Then, the probability curve of rib fractures is a function of the viscous criterion and derives from a study on human cadaver mid-sternum available in the literature. Twelve impact conditions of rigid projectiles are applied to a SEBS gel block and are replicated on the human torso model mid-sternum. A statistical analysis is performed by virtue of a Spearman's correlation matrix in order to identify relations between experimental measurements and the viscous criterion evaluated numerically. The determination of both statistical significances and correlation coefficients results in several strong correlations between experimental measurements and the viscous criterion evaluated numerically. These relations imply the establishment of transfer functions between experimental metrics on the gel block (the maximum gel wall displacement and a gel wall displacement - rate of displacement based metric) and the probability of rib fractures. Finally, these correlations constitute a primary and an up-and-coming predicting tool for the risk of rib fractures.

A three-dimensional geometric quantification of human cortical canals using an innovative method with micro-computed tomographic data.

The complex architecture of bone has been investigated for several decades. Some pioneer works proved an existing link between microstructure and external mechanical loading applied on bone. Due to sinuous network of canals and limitations of experimental acquisition technique, there has been little quantitative analysis of three-dimensional description of cortical network. The aim of this study is to provide an algorithmic process, using Python 3.5, in order to identify 3D geometrical characteristics of voids considered as canals. This script is based on micro-computed tomographic slices of two bone samples harvested from the humerus and femur of male cadaveric subject. Slice images are obtained from 2.94 µm isotropic resolution. This study provides a generic method of image processing which considers beam hardening artefact so as to avoid heuristic choice of global threshold value. The novelty of this work is the quantification of numerous three-dimensional canals features, such as orientation or canal length, but also connectivity features, such as opening angle, and the accurate definition of canals as voids which ranges from connectivity to possibly another intersection. The script was applied to one humeral and one femoral samples in order to analyse the difference in architecture between bearing and non-bearing cortical bones. This preliminary study reveals that the femoral specimen is more porous than the humeral one whereas the canal network is denser and more connected.

Experimental study of the strain rate dependence of a synthetic gel for ballistic blunt trauma assessment.

The mechanical characterization of a polymer gel used as reference backing material for blunt ballistic impact interpretation is performed at room temperature from quasi-static (0.002s-1) up to high strain rates (1500s-1). As very high strain tensile tests (350%) are conducted, an appropriate gripping device and particular strain measurement techniques are used, as well as high strain compressive tests (80%) based on retro lighting imaging. One major challenge is to carry out reliable compressive tests at high strain rates with polymeric split Hopkinson pressure bars using high-speed imaging and specific signal processing software. These mechanical tests provide a primary response to the strain rate dependence of the hyperelastic material behavior. Indeed, the material exhibits a higher stress response when the strain rate increases. Moreover, dynamic compression tests highlight a larger radial strain propagating along specimen axis with higher strain rates. This preliminary study on the characterization of the gel's mechanical behavior, constitutes an interesting step for an evaluation of human surrogate material. The extensive constitutive law can therefore be implemented for numerical simulations, with an aim of impact biomechanics analysis and body armor assessment.

Experimental study of the expansion dynamic of 9 mm Parabellum hollow point projectiles in ballistic gelatin.

We study in this paper the expanding behaviour of hollow point 9 mm Parabellum projectiles (Hornady XTP(®) and Speer Gold Dot(®)). We defined a deformation rate that takes into account both the diameter increase and the length reduction. We plotted the behaviour of this parameter versus impact velocity (we refer to this curve as the expanding law). This expanding law has been plotted for different gelatin weight ratios and different gelatin block lengths. We completed our experiments with a set of high speed movies in order to correlate the deceleration to the state of expansion and size of the temporary cavity. Our results pointed out that full expansion is reached shortly after the projectile fully penetrates the gelatin. This result shows that the key point to accurately simulate human body interaction with a hollow point projectile is to accurately simulate the interface (skin, skull, clothes thoracic walls). Simulating accurately organs is only an issue if a quantitative comparison between penetration depths is required, but not if we only focus on the state of expansion of the projectile. By varying the gelatin parameters, we discovered that the expanding law exhibits a velocity threshold below which no expansion occurs, followed by a rather linear curve. The parameters of that expanding law (velocity threshold and line slope) vary with the gelatin parameters, but our quantitative results demonstrate that these parameters are not extremely critical. Finally, our experiments demonstrate that the knowledge of the expansion law can be a useful tool to investigate a gunshot in a human body with a semi-jacketed projectile, giving an estimation of the impact velocity and thus the shooting distance.

Evaluación de la neurotoxicidad de la indometacina administrada en forma crónica en el espacio intratecal de ratas / Assessment of the neurotoxicity of indometacine administered chronically in the intrathecal space in rats

Objetivos: Evaluar la posible neurotoxicidad de la indometacina por vía intratecal.Material y métodos: Un estudio prospectivo, doble ciego se llevó a cabo en 10 ratas adultas empleando una preparación diseñada a infundir continuamente 20 µg . m l- 1 de indometacina a través de un catéter en el espacio subaracnoideo usando una microbomba implantada, por 11 días. Otro grupo de 10 ratas recibió infusiones similares de solución salina al 0,9 por ciento, por el mismo periodo.Resultados: Todos los animales sobrevivieron el periodo de observación sin alteraciones neurológicas ni de comportamiento.A la autopsia, estudios macroscópicos y microscópicos no demostraron lesiones neurotóxicas definitivas. Dos ratas en cada grupo tuvieron en la médula espinal signos de inflamación crónica inespecífica. En un espécimen del grupo que recibió indometacina, se notó leve congestión vascular en la pia; estos hallazgos se adjudican a la presencia del catéter, que actuaría como cuerpo extraño.Conclusiones: Varios estudios realizados en preparaciones de la médula espinal han demostrado una función antálgica de un número de los AINEs a nivel del sistema de receptores NMDA y de prostaglandinas medulares. Los estudios aquí reportados demostraron la ausencia de neurotoxicidad específica producida por la infusión intratecal continua de indometacina durante once días (AU)

Chicken ovalbumin upstream promoter-transcription factor, hepatocyte nuclear factor 3, and CCAAT/enhancer binding protein control the far-upstream enhancer of the rat alpha-fetoprotein gene.

We have further characterized the most distal of the three alpha-fetoprotein (AFP) enhancers required for expression of the AFP gene in fetal hepatocytes and yolk sac endodermal cells. Almost total rat AFP enhancer 3 (E3) activity is driven by a 160-bp fragment at -6 kb containing three target regions for nuclear proteins that cooperate to stimulate transcription from the AFP and the thymidine kinase promoters in HepG2 hepatoma cells. Region 1, recently shown to be crucial for correct function of the enhancer in liver of transgenic mice, is recognized by two sets of transcription factors that bind to partly overlapping sites, 1a and 1b, in a noncooperative and nonexclusive manner. Site 1a contains a motif, AGGTCA, which is recognized by chicken ovalbumin upstream promoter transcription factors (COUP-TFs), but not by hepatocyte nuclear factor 4. Hepatocyte nuclear factor 3 (HNF3) and CCAAT/enhancer binding protein (C/EBP), which bind to regions 2 and 3, respectively, are likely responsible for the liver-specific E3 action. They play a key role by acting in synergy. The participation of nuclear receptors such as COUP-TFs, with C/EBP and HNF3, in the tight control of the distal AFP enhancer is a new, and perhaps key, step toward understanding the regulation and function of this enhancer, which may remain active throughout development.