ABSTRACT
A new compound, indoxyl-beta-D-glucuronide, was assessed as a substrate for the rapid detection of Escherichia coli in urine. Incorporation of this compound into MacConkey agar allowed the direct differentiation of E. coli as deep blue colonies distinct from lactose and nonlactose fermenters. The sensitivity was 88 to 90%, and the specificity was 100%.
Subject(s)
Escherichia coli/isolation & purification , Glucuronates , Indoles , Urine/microbiology , Culture Media , Escherichia coli/metabolism , Glucuronidase/metabolism , HumansABSTRACT
Several strains of gram-negative bacteria (seven genera, eight species) isolated from patients with urinary tract infections were found to hydrolyze myeloma immunoglobulin A (IgA) protein. Human IgG and IgM and colostrum IgA were not degraded by these organisms. Examination of cleavage digests showed two fragments of different electrophoretic mobilities, with antigenic reactivity and sodium dodecyl sulfate polyacrylamide gel electrophoresis profiles consistent with their identification as Fc and Fab components. The immunoelectrophoresis patterns of cleavage digests suggested that the proteases responsible for this hydrolysis may be dissimilar in the specificity of their IgA cleavage sites.
Subject(s)
Gram-Negative Bacteria/enzymology , Peptide Hydrolases/isolation & purification , Serine Endopeptidases , Urinary Tract Infections/microbiology , Animals , Cricetinae , Gram-Negative Bacteria/isolation & purification , Humans , Immunoelectrophoresis , Immunoglobulin A/analysis , Molecular Weight , Myeloma Proteins/analysis , Species SpecificityABSTRACT
The urease activity of Ureaplasma urealyticum was found to be located in the cytoplasmic fraction and consisted of multiple stable enzyme forms representing at least four biotypes.
