Biosynthesis of metallic nanoparticles by bacterial cell-free extract.

The biosynthesis of metallic nanoparticles (MNPs), encompassing noble metals, metal oxides, and sulfides, has gained significant attention in recent years due to their unique properties and wide-ranging applications. However, traditional chemical synthesis methods often involve extreme conditions, harsh chemicals, and negative environmental impacts. Consequently, developing a simple, non-toxic, and eco-friendly approach for MNP synthesis is paramount. One promising method that addresses these concerns is using a bacterial cell-free extract (CFE) as a mediator for biosynthesis. Compared with other biosynthesis production methods, the purification process of MNPs synthesized using bacterial CFEs is much simpler, and CFE production is easier to standardize and reproduce. Bacterial CFEs are rich in various biomolecules, including proteins, enzymes, and peptides, which serve as both reducing and oxidizing agents during MNP formation. These biomolecules act as capping agents, contributing to the stability and monodisperse nature of MNPs. Using bacterial CFEs for MNP synthesis offers several advantages. Firstly, it aligns with eco-friendly practices as a biosynthesis approach. The non-toxic process minimizes environmental damage. Additionally, bacterial CFEs are cost-effective, making large-scale production economically viable. This review provides insights into these mechanisms, highlighting the role of CFE biomolecules and their impact on MNP characteristics. It also investigates the correlation between synthesis parameters, morphologies, and physical, chemical, and biological properties, allowing for tailored MNP design through the biosynthesis conditions. Despite its advantages, bacterial CFE-mediated biosynthesis faces challenges. This review addresses these challenges and discusses potential solutions. It also explores future perspectives, emphasizing areas for further investigation and innovation. In summary, using bacterial CFEs to synthesize MNPs offers significant advantages over other methods. It ensures eco-friendly, non-toxic, and cost-effective production. The review emphasizes the mechanisms and biomolecules involved, showcasing the potential for tailored MNP design. It also addresses challenges and prospects, paving the way for advancements in this field. Furthermore, the originality of this work lies in the exploitation of bacterial CFEs as a highly efficient and scalable platform for MNP synthesis.

Cassava wastewater valorization for the production of biosurfactants: surfactin, rhamnolipids, and mannosileritritol lipids.

The global production of cassava was estimated at ca. 303 million tons. Due to this high production, the cassava processing industry (cassava flour and starch) generates approximately ca. 0.65 kg of solid residue and ca. 25.3 l of wastewater per kg of fresh processed cassava root. The composition of the liquid effluent varies according to its origin; for example, the effluent from cassava flour production, when compared to the wastewater from the starch processing, presents a higher organic load (ca. 12 times) and total cyanide (ca. 29 times). It is worthy to highlight the toxicity of cassava residues regarding cyanide presence, which could generate disorders with acute or chronic symptoms in humans and animals. In this sense, the development of simple and low-cost eco-friendly methods for the proper treatment or reuse of cassava wastewater is a challenging, but promising path. Cassava wastewater is rich in macro-nutrients (proteins, starch, sugars) and micro-nutrients (iron, magnesium), enabling its use as a low-cost culture medium for biotechnological processes, such as the production of biosurfactants. These compounds are amphipathic molecules synthesized by living cells and can be widely used in industries as pharmaceutical agents, for microbial-enhanced oil recovery, among others. Amongst these biosurfactants, surfactin, rhamnolipids, and mannosileritritol lipids show remarkable properties such as antimicrobial, biodegradability, demulsifying and emulsifying capacity. However, the high production cost restricts the massive biosurfactant applications. Therefore, this study aims to present the state of the art and challenges in the production of biosurfactants using cassava wastewater as an alternative culture medium.

Remediation of soil contaminated with a commercial diesel-biodiesel blend (B12): A microcosm evaluation on the effects of (in)organic amendments.

Bioremediation of fuel-contaminated soils largely depends on microbial activities, which might be stimulated using (in)organic amendments. Attenuation of a diesel-biodiesel blend (B12) was investigated in microcosms during 93 days. Soil was spiked with B12 (5%, m m-1) and, in addition to contaminated Controls (unamended), soils received compost (COB), soybean hulls (SHB), or NPK fertilizer (IB) to reach a ~20:1 carbon-to-nitrogen (C:N) ratio regarding B12-carbon content. Effects of treatments on B12 attenuation, soil respiration, heterotrophic and B12-utilizing bacteria, pH, organic-C, nitrogen contents, and phytotoxicity, were evaluated. After 20 days, diesel range organics analysis indicated 58, 48, 45, and 43% attenuation in Controls, SHB, IB, and COB, respectively. Final dissipation reached 90, 86, 72, and 60% in Controls, COB, IB, and SHB. Compost and soybean hulls appeared as preferential substrates for microorganisms. Although microbial activity (soil respiration) was 39 and 22% higher than Controls in COB and SHB, amendments postponed attenuation. Amendments transiently affected bacterial numbers as compared to Controls; however, these effects were not related to attenuation levels. pH of the contaminated soils (~7.0) dropped to 6.1 in IB, whereas pH values were between 6.7 and 7.6 in other treatments. Organic-N and Kjeldahl-N decreased during incubations, indicating net N mineralization and subsequent nitrification, although N losses could occur. Organic-C, initially higher in SHB and COB, decreased in all treatments; however, more prominent losses in COB and SHB suggest amendments were preferentially used by microorganisms. Phytotoxicity was improved in Controls; however, it was not associated with attenuation levels in amended treatments, possibly owing to formation of toxic products and B12 sorption/desorption. In IB, decreased microbial activity, delayed attenuation, and remarkable phytotoxicity were due to excessive fertilization. Therefore, intrinsic soil conditions were adequate for B12 attenuation, without the need for nutritional inputs. Results also demonstrate that toxicity bioindicators are relevant to monitor remediation.